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Volume 61 (1984 - 198･･･
- Issue 6 Pages 399-
- Issue 5 Pages 327-
- Issue 4 Pages 235-
- Issue 2-3 Pages 83-
- Issue 1 Pages 1-
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Volume 61, Issue 6
Displaying 1-6 of 6 articles from this issue
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Yasuaki HOTTA, Yoshio MABUCHI, Nakazo WATARI1985 Volume 61 Issue 6 Pages 399-421
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe B-cell granules in the dog pancreatic islets are characterized by unique crystalline bar-like shapes under a transmission electron microscope, but their three-dimensional structure could not be observed by conventional transmission electron microscopy. In this study, the authors analyzed the three-dimensional structure of these crystalline B-cell granules using a scanning electron microscope and a method for the reconstruction from the electron micrographs of serial sections. Under transmission electron microscopy, electron micrographs were taken from tissues cut into serial sections, and the whole shapes of the core of B-cell granules were reconstructed. On the other hand,2.5% glutaraldehyde-fixed tissues were treated with the tannin-osmium method of Murakami, freeze-fractured, coated with Pt-Pd slightly, and then electron micrographed with scanning electron microscope.
From these observations, at least two kinds of three-dimensional shapes were recognized: one is a very peculiar shape obtained as if by slicing the cylinder obliquely. However, the cut surface was oblong, with fairly long, parallel sides. The second shape is that of a rhombic hexahedron, which has been reported already by the authors in the human pancreatic islet B-cells. Moreover, the fine particles were observed on the surface of the cores of B-cell granules, although their characteristics could not be ascertained.View full abstractDownload PDF (4603K) -
Shin-ichi IWASAKI, Ken MIYATA1985 Volume 61 Issue 6 Pages 423-435
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSScanning electron microscopic observations were performed to investigate the structure of the lingual papillae and the ultrastructure of the real surface of lingual dorsal epithelial cells in guinea pigs.
Filiform papillae were distributed over the entire dorsal surface of the tongue except for the narrow radix zone. Fungiform papillae were scattered among these filiform paillae. In the posterior area of the fore-tongue, many coronal papillae, whose shape was different from both the filiform and fungiform papillae, were distributed.
Higher magnification of the lingual dorsal epithelium revealed that prominent microridges and distinctive cell marginal thickening occurred widely in the basal area of the filiform papillae, inter-papillar area and lingual radix area. On the epithelial surface of the upper part of the filiform papillae, only fine pits and hollows were found. Microridges forming network and parallel patterns were widely distributed on the coronal papillar surface.View full abstractDownload PDF (2799K) -
Shin-ichi IWASAKI, Koichi SAKATA1985 Volume 61 Issue 6 Pages 437-449
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSScanning electron microscopy was used to investigate the ultrastructure of the lingual dorsal epithelial cells and sensory organs of the bullfrog. The specimens were prepared by a method involving osmium post-fixation and acid-treatment to remove extracellular material adhering to the surface of the tongue.
Over almost the entire dorsal surface except the anterior margin and posterior protrusions, filiform papillae consisting of a large number of non-ciliated cells with microridges and a small number of ciliated cells were compactly distributed, while fungiform papillae were scattered among these filiform papillae. A round sensory disk was located on the top of each fungiform papilla. The surface of the disk was widely covered with a honeycomb structure, and a small number of cells with microvilli on their surfacewere scattered amongst the honeycomb structure. Each sensory disk was encircled by a ciliated cell band.View full abstractDownload PDF (3203K) -
Shuji YAMASHITA, Kenjiro YASUDA1985 Volume 61 Issue 6 Pages 451-463
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA simple and rapid method was developed to purify monospecific antibody from heterospecific antisera utilizing “protein blotting”. Proteins were separated on a polyacrylamide gel. They were then transferred to nitrocellulose paper with an electric force, and the nitrocellulose strip corresponding to the relative mobility of antigen was removed and treated with heterospecific antisera. Monospecific antibody was successively eluted with acidic buffer from the strip. By employing this technique, monospecific antibodies against rat parotid amylase, parotid deoxyribonuclease (DNase), and albumin were purified from a mixture of these antisera.
Amylase, DNase and albumin were localized in both rat parotid gland and liver by use of these purified monospecific antibodies in the immunohistochemical method.View full abstractDownload PDF (2188K) -
Koji KAMI1985 Volume 61 Issue 6 Pages 465-481
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe ultrastructure of human diabetic placental capillaries diagnosed as a White's Class A, were investigated. The capillaries have a continuous endothelium rich in micropinocytotic vesicles, osmiophilic granules, and microfilaments, as well as increased number of cellular organellae. Many endothelial cells are enlarged with a subsequent reduction in the size of the lumen. Luminal endothelial extensions frequently show projections like apocrine flaps and marginal folds, although these cells are connected by zonulae occludentes characterized by 2 to 4 focal regions of membrane contact. The endothelial cells are provided with pseudopodial extensions which may be responsible for the exchange of materials between the vessels, vinous stroma, and vessel lumen, as well as for intermittent constriction. The basal laminae were found to be both unilaminar and multilamellar. Numerous osmiophilic granules are seen in the endothelial cells and pericytes as well in vinous epithelial- and stromal cellular components. These may be concerned with diplacental transport at the level of the fetal capillary, which is controlled by both the cytoplasm of the endothelial cells and by other cellular elements. The significance of the morphological changes between the endothelial cells and pericytes is discussed.View full abstractDownload PDF (4514K) -
Isumi TODA, Shigenori OKADA, Satoshi NAKAMICHI, Kengo KAJIWARA1985 Volume 61 Issue 6 Pages 483-491
Published: 1985
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA rare anomaly, in which the left-sided inferior vena cava was present, accompanied by the single column type of azygos venous system, was found in an 86-year-old Japanese female among cadavers for student dissection. The vein in this case appeared to be represented as a persistence of the left postrenal segment of the fetal inferior venae cavae, without any visceral transposition. Accordingly, this case may belong to type A-II-b-2 of Edwards'classification (1951), type C of Reis et al. (1959), I-type C of Chuang et al. (1974), type III of Takemoto et al. (1978), and type II of Yoshida et al. (1981), of variations of the inferior vena cava. Since both the hemiazygos and the accessory hemiazygos veins were iacking in the azygos venous system, this pattern belongs to the single column type of Seib (1934).View full abstractDownload PDF (1596K)
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