Okajimas Folia Anatomica Japonica
Online ISSN : 1881-1736
Print ISSN : 0030-154X
ISSN-L : 0030-154X
Volume 66, Issue 2-3
Displaying 1-7 of 7 articles from this issue
  • Masachika SENBA, Kioko KAWAI
    1989 Volume 66 Issue 2-3 Pages 61-67
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Yellow-brown bodies were observed in the sinusoids of lymph node and histiocytes. The authors confirmed immunohistochemical reactivity of lysozyme, alpha-1-antichymotrypsin, S-100protein, alkaline phosphatase, and acid phosphatase in non-phagocytic and phagocytic histiocytes which contained yellow-brown bodies. Phagocytic histiocytes (histiocytes with yellow-brown bodies)were not reacted with lysozyme, alpha-1-antichymotrypsin, S-100 protein, alkaline phosphatase, and acid phosphatase. On the other hand, non-phagocytic histiocytes were reacted with lysozyme, alpha-1-antichymotrypsin, S-100 protein, alkaline phosphatase, and acid phosphatase.
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  • Harumichi SHINOHARA, Yoshifumi FUKUO, Takeshi MATSUDA
    1989 Volume 66 Issue 2-3 Pages 69-79
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    The peritoneal surface of the golden hamster diaphragm was examined for closed lymphatic stomata by scanning electron microscopy (SEM) and serial sectioning. Closed stomata were absent on SEM but present on serial sectioning. Some closed stomata in the serial sections were reconstructed using a computerized image analysis system. They were less than 10μm in diameter and consisted of an outer mesothelial margin and an inner lymphatic wall. The lymphatic wall was formed by several endothelial cells adjoined with various junctional abutments. The discrepancy of results between SEM and serial sectioning, and the functional aspects of lymphatic stomata are discussed.
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  • Light microscopy and three-dimensional image analysis
    Kazuhiro YAMASHITA, Fumio MORITA, Yoshinori OTSUKI, Sumiko MAGARI
    1989 Volume 66 Issue 2-3 Pages 81-97
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    The distribution and structure of the arachnoid granulations and villi in the region of the sella turcica in human adult brains were observed under light microscopy. In order to study the interrelationships between the arachnoid projections, hypophysis, cavernous sinus, and sella turcica, we performed a three-dimensional reconstruction and analysis of the region with the use of a digital image-processing method. Arachnoid projections penetrating the dura were villous when they were few in number, but more frequently granular when they were numerous. No relationship was observed between the type of projections and the age or the underlying disease of the subjects. Brains which showed no or few arachnoid granulations or villi had a small and deep hypophyseal fossa with a poorly-developed intercavernous sinus or venous plexus. Three-dimensional image analysis revealed that arachnoid granulations and villi form a maze in the dura or connective tissue between the venous plexuses.
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  • Shigemitsu YOSHIDA, Hayato OHSHIMA, Shigeo KOBAYASHI
    1989 Volume 66 Issue 2-3 Pages 99-111
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Vascularization of the enamel organ was demonstrated in the developing upper first molar teeth of rats from the 19-day embryo to 5 days after birth employing the vascular casting/scanning electron microscope method. Capillaries were first observed in the enamel organ at the 21-day embryo. By that time, with the beginning of differentiation of the inner enamel epithelium into ameloblasts, mesenchymal cells situated in close proximity to the inner enamel epithelium had begun to differentiate into odontoblasts, but deposition of organic substances had not commenced. The occurrence of blood capillaries before the nutritional supply through the dental papilla was interrupted by the deposition of dentin and enamel, may possibly be due to the high nutritional requirements of the ameloblasts following differentiation from the inner enamel epithelium. With the advance of dentin and enamel formation, many capillaries entered the enamel organ and finally formed a flattened vascular network next to the stratum intermedium. These findings suggest that the capillaries in the enamel organ should be regarded as a change which affords a rapid and sufficient supply of metabolic substances necessary for amelogenesis. The newly developed capillaries in the enamel organ grew first by sprouting and later by loop formation.
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  • Etsuo ISHIDOH
    1989 Volume 66 Issue 2-3 Pages 113-131
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Detailed observations were made of the a. malaris in 11 adult common squirrel monkeys (Saimiri sciureus) utilizing the plastic injection method. The malar artery was well-developed in all 21 examples observed and arose medially from the infraorbital artery proximal to the entrance of the infraorbital canal and lateral to the obliquus inferior muscle. The a. malaris passed superomedially outside the periorbita on the orbital surface of the maxilla and curved medially in front of the muscle, where it gave rise to the infraorbital nerve, the periosteal, the inferior oblique muscular and the infraorbital marginal branches. However, these branches arose directly from the infraorbital artery in many cases. The malar artery ascended in the lower eyelid up to the bottom of the lacrimal sac, where it gave rise to the third palpebral branch and the medial inferior palpebral artery. This artery did not anastomose with peripheral branches of the supraorbital artery. The malar artery continued to pass superomedially behind the lacrimal sac and gave off the nasolacrimal canal and the lacrimal sac branches. However, the former arose in common with the latter in many cases. The malar artery finally ascended behind the medial palpebral ligament after giving off the dorsal nasal branch and the medial superior palpebral artery at the superior end of the lacrimal sac. Its main stream formed a strong communication with the dorsal nasal artery of the supraorbital artery. This communication in an arc was characteristic in the common squirrel monkey. The medial inferior and superior palpebral arteries were well-developed in all examples and formed distinct, inferior and superior palpebral arterial arches by anastomosing with the lateral fellows of the lacrimal, respectively. A large arterial ring surrounding the upper tarsus was constructed from the superior palpebral arterial arch, branches of the lateral superior palpebral artery and the palpebral branch of the supraorbital artery.
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  • Toshikazu NISHIMURA, Norio KAWAI, Ichiro ICHIHARA
    1989 Volume 66 Issue 2-3 Pages 133-143
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    The behavior of simian virus 40 (SV40) injected into the vascular system was investigated in the rat glomerulus. The kidney was perfused via the abdominal aorta with a serum-free culture medium for 5 min, with PBS solution containing SV40 and then with the same medium for 15min at 37°C. In the glomeruli, SV40 particles were detected in the lumen of the eapillaries, fenestrations of endothelial cells and lamina rara interna of the glomerular basement membrane. They were also found in the mesangial matrix and mesangial cells. Invaginations of their membrane were observed on several surface areas where SV40 particles were localized close to the surface. Similarly, when the particles were injected into the tail vein, they were detected in the lamina rara interna, the mesangial matrix, and in vacuoles of mesangial cells at 2 h after the injection. These results indicate that SV40 particles migrate from the vascular system into the mesangial matrix, and are then endocytosed in vivo by mesangial cells.
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  • Yasukazu NAGATO, Masaki SEKIGUCHI, Tsuyuka KUSHIDA, Hiroshi KUSHIDA, K ...
    1989 Volume 66 Issue 2-3 Pages 145-151
    Published: 1989
    Released on J-STAGE: September 24, 2012
    JOURNAL FREE ACCESS
    Semithin sections embedded in water-miscible methacrylates were used for the study of fine structures of cells and tissues in the central nervous system by light microscopy instead of the conventional paraffin sections. This method used a water-miscible methacrylate mixture consisting of 2-hydroxypropyl methacrylate (HPMA), Quetol 523 and methyl methacrylate (MMA)as an embedding medium. The mixture had a low viscosity, was easy to handle and penetrated readily and completely into the specimen, producing a homogeneous block from which it was easy to make sections 1.5 μm thick. Staining could be localized far more precisely in these setions than in paraffin sections owing to the thickness of the semithin sections and to the excellent structural preservation of cellular components.
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