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Volume 72 (1995 - 199･･･
- Issue 6 Pages 285-
- Issue 5 Pages 235-
- Issue 4 Pages 185-
- Issue 2-3 Pages 51-
- Issue 1 Pages 1-
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Volume 72, Issue 4
Displaying 1-5 of 5 articles from this issue
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Hiroshi MORIYAMA, Kazuyuki SHIMADA, Noboru GOTO1995 Volume 72 Issue 4 Pages 185-189
Published: October 20, 1995
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSNeuron, Morphometry, Ganglion, Human, Peripheral nerve Summary: The geniculate ganglia (GG), the submandibular ganglion (SG), the cervical spinal ganglion (CSG) and the superior cervical ganglion (SCG) are clinically important. But details of these ganglia have not been included in textbook descriptions. We conducted research to bring precise figures to light. We prepared serial sections of these ganglia in five Japanese adults after precise dissections to count the numbers, measure the perimeters and the areas, and calculate the circularity ratios (CRs) of the ganglion cells. The numbers of neurons were in the following order: SCG>CSG>SG>GG, and the average neuronal areas in the following order: CSG>GG>SG>SCG. Average neuronal CRs were around 0.90 in all ganglia. In other words, the morphometric evaluation showed that neurons in such ganglia are practically circular in shape. Our morphometry thus reveals that the neurons in these ganglia are sensory neurons in the broad definition of the word from a quantitative point of view.View full abstractDownload PDF (1823K) -
Iwao SATO, Ryuji UENO1995 Volume 72 Issue 4 Pages 191-200
Published: October 20, 1995
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe distribution of collagen fibers of rat masticatory muscles during the postnatal period (two weeks), was investigated by electrophoresis and immunohistochemistry. At these stages, the myosin of rat masticatory muscles displays specific electrophoretic patterns. Comparison of the myosin patterns of these muscles allows their identification.
1) Analysis by SDS-PAGE indicated that one of three weakly reactive stainable proteins with lower mobility than the heavy chain of myosin disappeared from the temporal muscle on day 13, as compared with other masticatory muscles. However, in histochemical analysis of the muscle fibers, the reaction specific for succinic dehydrogenase (SDH) activity was strong, and the fibers on day 13 could be classified into two types with respect to SDH activity. By contrast, on day 0, the fibers were classified into two types with respect to myosin ATPase activity.
2) Immunohistochemical analysis indicated that the distribution of the components of the extracellular matrix in the epimysium (type I collagen), perimysium (type I collagen, fibronectin, and laminin) and endomysium (type III collagen, fibronectin, laminin, and tenascin) was related to the metabolic capacity on days 12 to 13. The variability in the types of myosin and in proteins of the extracellular matrix might be important during the development of rat masticatory muscles.View full abstractDownload PDF (3236K) -
Toshiyuki KOUNO, Sadahiro WATANABE, Takako NOMURA, Hideki MORI, Junzo ...1995 Volume 72 Issue 4 Pages 201-219
Published: October 20, 1995
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSBladder, Epithelial cell, HRP, Endocytosis Summary: Transitional epithelium of mammalian urinary bladder is a functional barrier which prevents passage of urine into the submucosa. In a previous study, we found uptake by the epithelial cells of horseradish peroxidase (HRP) instilled into the mouse bladder lumen. In the present study, the ability of bladder epithelial cells to take up HRP, which was visualized by the diaminobenzidine (DAB) reaction, was compared among several mammalian species by light and electron microscopy.
In mouse, rabbit and dog bladder, the superficial cells of the transitional epithelium showed positive reactions for HRP, while cells in lower epithelial layers were DAB-negative. Some of the reaction products could be seen as a number of granules not surrounded by a membrane. The intercellular space of the epithelium was DAB-negative.
In rat and guinea pig bladder, however, all urothelial cells showed negative reactions for HRP indicating that no uptake of HRP had been occurred in these animals.
These results indicate that the uptake of HRP by the superficial cells of the bladder epithelium differs among mammalian species, indicating some functional differences in their superficial cells.View full abstractDownload PDF (5598K) -
Immunohistochemical Investigations of Parvalbumin Localization in the Skeletal Muscle Fibers of RatsHiromichi ANNOH, Tetsuo INOKUCHI, Keiske OHTA, Mitsugu WAKIMOTO, Tamak ...1995 Volume 72 Issue 4 Pages 221-226
Published: October 20, 1995
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSParvalbumin (PA) is a water soluble, low-molecular weight, calcium-binding protein which has been thought to be involved in the relaxation of skeletal muscle fibers. Although it is well known that PA concentrations are higher in fast twitch fiber than slow twitch fiber, the localization of PA within the cytoplasm of single muscle fibers is still unknown. The present study, therefore, was undertaken to clarify the PA localization by immunohistochemical methods using the confocal laser scanning microscope (CLSM) and transmission electron microscope (TEM).
Wistar strain male rats were fixed by vascular perfusion with 4% paraformaldehyde solution, and the extensor digitorm longus muscle was dissected out. For fluorescent antibody examination, these specimens were quickly frozen in melting isopentan and sections were cut using cryestat at-25°C. These sections were incubated in anti-PA and anti-Troponin (TR) respectively, and then exposed to Texas-Red- and FITC-labelled secondary antibodies. For TEM study, the pre-embedding method was used. Fluorescent immunohistochemical study has clearly shown that both PA and TR are located intimately in the I-band of the skeletal muscle fibers. The finding by the immunofluorescent study correlated well with those which have been seen at the ultrastructural level. The fact that PA is located in close proximity to TR is considered to be very reasonable when we consider it in terms of the muscular contraction-relaxation cycle.View full abstractDownload PDF (1901K) -
Koji KAMI, Noriaki SATO, Toshiyuki MORITA, Isamu ISHIWATA, Masakazu IS ...1995 Volume 72 Issue 4 Pages 227-234
Published: October 20, 1995
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSA 91 kDa protein, sharing antigenicity with a pregnancy-associated antigen, was isolated from the ascitic fluid of a patient suffered from the gynaecological malignant neoplasms. It was revealed in the human placenta by immunocytochemical techniques, and found to be localized in the stroma, including a fibrous network, fibroblasts and the Hofbauer cells of the terminal and stem villi in the first trimester. Immunoreactive staining was also found in macrophage-like cells, presumable the precursor of the Hofbauer cell, in the stem villi of placenta. It is assumed that macrophage-like cells containing the protein would be originated from the extraembryonic mesoderm or blood vessels, and then migrated to the stroma of terminal villi. During the second trimester, the protein was observed in numerous vesicles among the syncytial trophoblasts. The density of these immunoreactive vesicles and the cytoplasm of Hofbauer cells was rapidly increased with gestational stages, whereas it was gradually reduced towards the late-second trimester. The highest labellings occurred at around 17 weeks or later. On the other hand, at term, the protein was mainly observed in numerous villous blood vessels including endothelial cells. In the light of these results, a hypothesis concerning mechanisms of transplacental permeability of the protein by the placental barrier is discussed further.View full abstractDownload PDF (2733K)
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