Summary: In the rat subfornical organ (SFO), lectin-binding glycoconjugates were histochemically examined by 9biotinylated lectins using a streptavidin-biotin peroxidase system. The strong or moderate bindings of
Canavalia ensiformis (Con A),
Lens culinaris (LCA) and
Phaseolus vulgaris erythroagglutinin (ePHA) indicated overall distributions of high mannose, intermediate, hybrid N-linked, and non-bisected and bisected, bi/triantennate N-linked complex oligosaccharides in the rat SFO. Nonsialylated terminal N-acetylglucosamines were detected throughout this organ, as revealed by its stainabilities with
Triticum vulgaris (WGA) and
Limax flavus (LFA). In this organ,
Ricinus communis (RCA-1) specifically bound to vessel-associated structures, whereas
Arachis hypogaea (PNA) reacted with selected neurons in the central and rostral regions of this organ.
Dolichos biflorus (DBA) and
Ulex europaeus (UEA-1) did not stain any histologic structures in the rat SFO. The results obtained in this study provide a basis for comprehensive analyses of glycoconjugates in the rat SFO.
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