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Volume 77 (2000 - 200･･･
- Issue 6 Pages 189-
- Issue 5 Pages 137-
- Issue 4 Pages 93-
- Issue 2-3 Pages 39-
- Issue 1 Pages 1-
Predecessor
Volume 77, Issue 4
Displaying 1-5 of 5 articles from this issue
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Bi ZHANG, Masahiro MIURA, Rui-Cheng JI, Yuushi MATSUMOTO, Shigetaka YA ...2000 Volume 77 Issue 4 Pages 93-107
Published: October 20, 2000
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe structural organization and fine distribution of lymphatic vessels in the periodontal tissues (gingiva, periodontium and alveolar process) were examined by light and electron microscopy using an enzyme-histochemical method. Whole mount preparations of periodontal membranes pealed from the teeth and cryostat sections of normal or decalcified tissues treated with EDTA were double-stained using 5'-nucleotidase (5'-Nase)-alkaline phosphatase (ALPase) and examined by light microscopy. This staining procedure allowed the lymphatic vessels in the periodontal tissue to be differentiated from blood vessels. Well-developed 5'-Nase-positive lymphatics were observed in the gingiva and periodontium. The histochemical aspects of 5'-Nase activity in lymphatic vessels are discussed in detail, with special reference to the supply of Mg++ ions. A network of 5'-Nase-positive lymphatics was observed in whole mount preparations of the periodontal membrane for the first time. This network was also observed in the tissue sections. More 5'-Nase-positive lymphatics were seen in the root area of the periodontium than in the cervical area.5'-Nase-positive lymphatics in residual tissue blocks remaining after cryostat sectioning and in whole mount preparations were highlighted with good contrast and resolution on backscattered electron images produced by scanning electron microsopy. Dense granular precipitations resulting from the 5'-Nase reaction were observed on the lumina' surface of the lymphatic endothelial cells as well as on the basal side but were absent in the blood vessels.View full abstractDownload PDF (7003K) -
Yasutomo IWAI-LIAO, Shunji KUMABE2000 Volume 77 Issue 4 Pages 109-118
Published: October 20, 2000
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSOptic and confocal laser scanning microscopy (CLSM) and the LsAB method were used to immunolocalize TGFa, EGF, FGF-2 and their related receptors, which are involved in the regulation of organogenesis in the mouse hypophysis. Internalization of the above receptors and the active proliferation of the presumptive adenohypophysis and neurohypophysis were observed during the mid-foetal stage. CLSM images were used to map the distribution of Cx32in the proliferating hypophysis, particularly between the closely apposed neuro- and adenohypophyses. Using conventional transmission electron microscopy, gap junctions were observed at the boundary of these structures. The results suggest that cell coupling via gap junctions may provide positional information that is then used to control the differentiation of the hypophyseal cells.View full abstractDownload PDF (4244K) -
Toshio HAYAKAWA, Masahiro ITOH, Takanori MIKI, Tsuyoshi KANETO, Hanako ...2000 Volume 77 Issue 4 Pages 119-124
Published: October 20, 2000
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSCells of origin of sympathetic fibers innervating the extraocular muscles were demonstrated in the cat superior cervical ganglion (SCG) using the horseradish peroxidase (HRP) technique. Each injection of HRP into the muscles was confirmed by labeling in the oculomotor, trochlear and abducens nuclei. In all cases retrogradely labeled neurons were found to be distributed widely to the ipsilateral SCG. Labeled newtons were round or oval in shape and majority of these were medium in size. There were some large-sized neurons which frequently showed to have long dendrites expanding rostrocaudally. The labeling was characterized by localization in the rostral two-thirds of the SCG after HRP injections into the medial, lateral and inferior and superior oblique muscles. However, the labeling was found in the whole of the SCG after HRP injections into the superior rectus and inferior oblique muscles. Compared with our previos studies demonstrating cells of origin of the sympathetic fibers in the facial and hypoglossal nerves, the present experiments showed quite different pattern of distribution of labeled neurons in the SCG.View full abstractDownload PDF (1976K) -
Shigeru INAFUKU, Men-dar WU, Masaru KIMURA, Meiho NAKAYAMA, Takashi NA ...2000 Volume 77 Issue 4 Pages 125-131
Published: October 20, 2000
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSThe morphology and immunohistochemistry of inducible nitric oxide synthase (iNOS) and nuclear factorkappa B (NF-KB) were studied on the spiral and vestibular ganglion of young and old ddy mice. The significant decrease in the number of the spiral ganglion cells and a significant expression of iNOS and NF-KB were observed in old mice. In contrast, in the vestibular ganglion of all animals examined, decrease in the number of the ganglion cells or expression of iNOS and NF-KB were not observed. Although the relevance of enzymatic systems for the protection of vestibular ganglion cells in old individuals from harmful oxidative stress increased with aging should be further clarified, lack of harmful stress due to nitric oxide (NO) may be one of the plausible reasons for that the vestibular ganglion cells were not decreased in number with aging, since iNOS was not detected in the vestibular ganglion cells in the animals tested in the present study.View full abstractDownload PDF (2259K) -
Ryuichiro YANO, Daisuke HAYAKAWA, Shoichi EMURA, Huayue CHEN, Yuki OZA ...2000 Volume 77 Issue 4 Pages 133-136
Published: October 20, 2000
Released on J-STAGE: September 24, 2012
JOURNAL FREE ACCESSTwo cases of the double inferior venae cavae (IVC) were found during the student dissection practice in 1997 in Gifu University School of Medicine.
On the first case (70-year-old male), the calibers of the right and left IVC were 15 mm and 13 mm, respectively. An anastomosis (4-mm caliber) ran obliquely from the left internal iliac vein to the right IVC. On the second case (86-yearold male), the calibers of the right and left IVC were 15 mm and 10 mm, respectively. We found no anastomosis between the right and left IVC. Each IVC was observed behind the ureter.
Both cases belong to Type BC of the classification of McClure and Butler (1925), that is based on the combinations of the right and left IVC, and on their location to the ureters. Both cases also belong to Type II-b-2 of the classification of Takemoto et al. (1978), that is based on the calibers of the right and left IVC and on the running course of the interiliac vein.
These are the first and second cases among 808 cadavers in Gifu University School of Medicine and the 93rd and 94th cases in Japan since 1901.View full abstractDownload PDF (1372K)
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