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Volume 85 (2008)
- Issue 4 Pages 111-
- Issue 3 Pages 79-
- Issue 2 Pages 43-
- Issue 1 Pages 1-
Predecessor
Volume 85, Issue 2
Displaying 1-5 of 5 articles from this issue
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Kin-ya KUBO, Naonori SAITOH, Yasutoku KOGAYA, Fumihiko IWAKU, Yukiko I ...2008 Volume 85 Issue 2 Pages 43-48
Published: 2008
Released on J-STAGE: November 22, 2008
JOURNAL FREE ACCESSThe bite raised condition decreases the number of neurons and increases the amount of glial fibrillary acidic protein in the hippocampus of aged SAMP8 mice. In the present study, we examined whether these effects differ between the dorsal and ventral hippocampus. In bite-raised SAMP8 mice, the number of neurons was significantly lower in the hippocampal CA1 and dentate gyrus (DG) subfields compared to control mice. In the bite raised condition, the number of neurons was significantly lower in both the dorsal and ventral CA3 subfields, and the number of glial fibrillary acidic protein-labeled astrocytes was increased in the CA1, CA3, and DG subfields, compared to control mice. These data suggest that in aged SAMP8 mice, the bite-raised condition enhanced aging processes in both the dorsal and ventral hippocampus.View full abstractDownload PDF (2749K) -
Keiji OKAMOTO, Kiyomi TAKADA, Rie IKEDA, Shigeo AIYAMA2008 Volume 85 Issue 2 Pages 49-56
Published: 2008
Released on J-STAGE: November 22, 2008
JOURNAL FREE ACCESSThis study was designed to examine whether or not phospholipid is contained in the secretory granules of the rat palatine gland acinar cells, and if present, to examine the movements of phospholipid in the secretory granules during postnatal development. The palatine glands of male Wistar rats aged 0 to 56 days were used. Acid-hematin staining showed a few positive acinar cells with a faint reaction in the acini on day 0, numerous positive cells with an intense reaction on day 7, a weakening reaction in the cells on day 14, and almost no reactivity on day 35 and after. In contrast, alcian blue staining showed acinar cells with a weak reaction on day 7, a gradual increase in the reaction from day 14, and the presence of many cells with an intense reaction on day 28 and after. Electron probe microanalysis (EPMA) revealed a higher density of phosphorus in samples on day 7 than on day 56. These findings suggest that developing rat palatine gland acinar cells contain phospholipid in the secretory granules, being particularly more conspicuous around postnatal day 7, but that the amount of phospholipid decreases as the cells change to mature mucous cells.View full abstractDownload PDF (5781K) -
Comparative morphological study on the lingual papillae and their connective tissue cores in rabbitsKouji NONAKA, Jin Hua ZHENG, Kan KOBAYASHI2008 Volume 85 Issue 2 Pages 57-66
Published: 2008
Released on J-STAGE: November 22, 2008
JOURNAL FREE ACCESSThe morphological structure of the lingual papillae and their connective tissue cores (CTC) in a rabbit were studied using LM and SEM and were compared to that of other animal species. Externally, the filiform papillae distributed on the anterior surface of the dorsal tongue were short and conical with a round base and had a flat area on their anterior upper half. The CTC of the conical filiform papillae had a roughly triangular plate-like structure with a round top. Several small round protrusions were found on both inclined planes of the triangle. Spearhead-like filiform papillae were distributed on the anterior edge of the lingual prominence and branched filiform papillae were on the posteriorly wide area of the prominence. These papillae on the prominence had a slightly ramified CTC that differed from that of the CTC of the conical filiform papillae distributed on the anterior tongue. Dome-like fungiform papillae were distributed among the conical filiform papillae of the anterior tongue and had a CTC with a roundish structure that was almost but, not quite spherical in appearance with 1 to 10 small round concave indentations for taste buds on their upper surface. The foliate papillae had approximately 15 parallel ridges separated by grooves. These ridges contained a parallel thin plate-like CTC exhibited after removal of the epithelium. The vallate papilla was comprised of a spherical central papilla and had a circular wall with a flower-like CTC almost resembling a carnation.
The stereostructure of the rabbit's filiform CTC are comparatively described as being morphologically in between those of rodents and those of the guinea pig and Japanese serow. Such evolution has probably occurred due to the species unique masticatory and gustatory needs and functions.View full abstractDownload PDF (6374K) -
Keiichi MORIGUCHI, Takamichi JOGAHARA, Takayuki KURIHARA, Jun IWAMI, N ...2008 Volume 85 Issue 2 Pages 67-72
Published: 2008
Released on J-STAGE: November 22, 2008
JOURNAL FREE ACCESSTannerella forsythensis (Bacteroides forsythus), an anaerobic gram-negative potential periodontal pathogens in the progression of periodontitis. T. forsythensis has unique bacterial protein profiles containing major proteins with apparent molecular weight of more than 200-kDa shown by sodium dodecylsulfate-polyacrylamide gel electrophoresis. It is also known to have a typical surface layer (S-layer) consisting of regularly arrayed subunits outside the outer membrane revealed by electron microscopy. On the other hand, electron microscopy showed that the best preservation of structure was obtained when cells were postfixed with OsO4, but this resulted in very low levels of gold particles labeling. Therefore, cells were applied to pieces of filter paper and freeze-substituted by plung-freezing in Liquid propane, substituted in methanol containing 0.5% uranyl acetate, and infiltrated with LR-White resin. We also examined the relation between high molecular weight proteins and S-layer in energy-filtering transmission electron microscopy (EF-TEM) to visualize 3,3’diaminobenzidene, tetrahydrochloride (DAB) reaction. The three-window method in electron spectroscopic images (ESI) of nitrogen (N) element, reflecting the presence of DAB moieties by the DAB reaction solution, horseradish peroxidase (HRP)conjugated secondary antibodies instead of immunogold particles obtained by the EF-TEM. The mapping patterns of net N element were restricted to the outermost cell surface.View full abstractDownload PDF (3833K) -
XiaoBo ZHU, Shogo MATOBA, Kenshiro HARA, Yasuhisa ANO, Shin-ichi KOBAY ...2008 Volume 85 Issue 2 Pages 73-77
Published: 2008
Released on J-STAGE: November 22, 2008
JOURNAL FREE ACCESSIt is suggested that pathogenic prion protein (PrPsc) may accumulate in peripheral nervous tissues in addition to the specified-risk materials. To assess the risk of PrPsc accumulation in peripheral tissues, the present study was designed to study the ratio of peripheral nervous tissues in tongues, skeletal muscles and intestines in 2-week- and 59-month-old cows. The nervous tissues could be detected by immunohistochemistry using antibody Neurofilament 150. After immunostaining with Neurofilament 150, the photographs taken from each section were applied for Scion Image software. Then, the ratio of nervous tissues in tongues, skeletal muscles and intestines were calculated. The highest ratio of peripheral nervous tissues was observed in intestines, the second highest ratio was found in tongues, and the lowest ratio was recognized in skeletal muscles in both 2-week- and 59-month-old cows. The ratio of nervous tissues in intestines was approximately 2.5 fold and 10 fold, compared to that in skeletal muscles in 2-week- and 59-month-old cows, respectively. These results suggest that intestines and tongues may have a higher risk of PrPsc accumulation, compared to skeletal muscles.View full abstractDownload PDF (1662K)
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