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Volume 87 (2010)
- Issue 4 Pages 155-
- Issue 3 Pages 75-
- Issue 2 Pages 33-
- Issue 1 Pages 1-
Predecessor
Volume 87, Issue 1
Displaying 1-5 of 5 articles from this issue
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Zhe Wu JIN, Young Gon KIM, Hee Chul YU, Gen MURAKAMI, Mineko FUJIMIYA, ...2010 Volume 87 Issue 1 Pages 1-4
Published: 2010
Released on J-STAGE: February 10, 2012
JOURNAL FREE ACCESSBoth M2A (D2-40) and c-KIT have frequently been used as markers of germ cell tumors. However, comparative studies of their immunoreactivities in human fetal gonads have been limited. Using horizontal semiserial whole-abdomen sections of 9 human abortuses at 12 weeks (2 males and 1 females; CRL 70-80 mm), 15 weeks (2 males and 3 females; 115-125 mm) and 18 weeks (1 female; 155 mm) of gestation, we identified germ cells in the testis and ovary on the basis of c-KIT immunoreactivity. M2A was also stained using near or adjacent sections. In 12-week fetuses, c-KIT immunoreactivity was weak, but M2A immunoreactivity was consistently strong. At 15 and 18 weeks of gestation, c-KIT immunoreactivity was found in most germ cells. M2A was expressed in Sertoli cells as well as in germ cells, but was not expressed in ovarian follicles undergoing meiosis. M2A appears to be a more useful marker of germ cells than c-KIT in human fetal testis and ovary at the second trimester. M2A is also reactive in fetal Sertoli cells, but its expression is consistently weaker than in germ cells.View full abstractDownload PDF (3380K) -
Yukio KATORI, Zhe Wu JIN, Tetsuaki KAWASE, Ki Hwan HONG, Gen MURAKAMI, ...2010 Volume 87 Issue 1 Pages 5-10
Published: 2010
Released on J-STAGE: February 10, 2012
JOURNAL FREE ACCESSImmunoreactivity of the calcium binging protein calretinin is often used as a marker of olfactory neurons. Although the immunoreactivity and density of olfactory neurons are known to change between developmental stages in the human fetus, previous descriptions have been limited to the olfactory epithelium and/or the nasal septum and have not included the entire nasal cavity. Using horizontal semi serial sections of heads of six mid-term fetuses (9-15 weeks of gestation), we examined the topographical anatomy of calretinin-positive olfactory neurons. By 9 weeks of gestation, the distribution of calretinin-positive cells reached levels inferior to the developing inferior meatus. By 12 weeks, concentrations in the inferior end had reached the level of the inferior meatus and the middle meatus carried abundant positive cells. However, by 15 weeks, calretinin positive cells were restricted to levels superior to the middle meatus and in the vomeronasal organ. Placode-derived cells are initially distributed antero-inferiorly along the nasal epithelium, but most lose their calretinin immunoreactivity. They might differentiate into the neuroendocrine cells embedded between nasal respiratory epithelial cells. The final differentiation of calretinin-positive cells was likely to require connection to the olfactory bulb and accessory bulb.View full abstractDownload PDF (4117K) -
Shogo INOUE, Baik Hwan CHO, Chang Ho SONG, Mineko FUJIMIYA, Gen MURAKA ...2010 Volume 87 Issue 1 Pages 11-16
Published: 2010
Released on J-STAGE: February 10, 2012
JOURNAL FREE ACCESSNeural crest-derived cells are believed to migrate into the fetal adrenal cortex from the medially-located hilus. However, there appears to be a paucity of observations of the migration and distribution of medullary cells in humans. In sagittal as well as horizontal sections of human fetuses between 9 and 16 weeks of gestation, we identified chromaffin, ganglion and Schwann-like cells in the developing adrenal gland using immunohistochemistry. Cells showing tyrosine hydroxylase (TH) immunoreactivity (i.e., candidate ganglion cells) entered the fetal cortex mainly from the medial half of the adrenal, but the path of entry also included the ventral, dorsal and caudal aspects. These cells displayed linear arrangements, forming a connection between the peripheral and central areas of the gland. S100 protein-immunoreactive cells (i.e., Schwann-like cells) accompanied most (but not all) of the TH-positive cells. The distribution of chromogranin A-immunoreactive cells (i.e., chromaffin cells) was similar to and overlapped with that of TH-positive cells. Chromogranin A-positive cells were observed around the aorta as well as in the adrenal. The entry of neural crest-derived cells does not appear to be restricted to a hypothetical medial hilus, but occurs widely around the cortex, with or without the accompaniment of Schwann-like cells. These cells advance in lines through the fetal cortex in a cord-like arrangement without destruction of the cortical architecture. Some of the TH-positive cells very likely express chromogranin A before entry into the adrenal.View full abstractDownload PDF (5973K) -
Abuduaini ABUDUREHEMAN, Shiro NAKAGAWA2010 Volume 87 Issue 1 Pages 17-23
Published: 2010
Released on J-STAGE: February 10, 2012
JOURNAL FREE ACCESSAfter a monocular injection of the cholera toxin B subunit (CTB) into the vitreous chamber of one eye, the retrogradely labeled retinopetal neurons were studied in the diencephalon of the Japanese monkey. The retrogradely transported tracer was visualized using the peroxidase antibody technique and an anti-cholera toxin antibody. The CTB-labeled nerve cell bodies were scattered in the periventricular nucleus of the hypothalamus, lateral hypothalamic area, and midline nuclei of the thalamus on both sides. In addition, a few retrogradely labeled nerve somata were observed in the most rostral portion of the lateral geniculate nucleus on the contralateral side.View full abstractDownload PDF (1738K) -
Natsuki SANO, Yuh HASEGAWA, Shigeki IIJIMA, Kazuto TERADA2010 Volume 87 Issue 1 Pages 25-31
Published: 2010
Released on J-STAGE: February 10, 2012
JOURNAL FREE ACCESSThe present study was undertaken to analyze the status of formation of the upper first molar in individuals suspected as having delayed eruption of the upper first molar.
The subjects of this study were 51 patients presenting to orthodontic specialists with delayed eruption of the upper first molars. The 95 teeth of these 51 patients were observed. The investigation of the tooth development status included evaluation of the tooth development stage on panoramic radiographs according to the method of Moorrees et al.
The upper first molars that showed delayed development were at the stage of initial cleft formation, reflecting a delay by 2.09 years in boys and 2.84 years in girls. In most cases, the delayed eruption was bilateral, and the development stage of the subject teeth did not differ between right and left sides.
The plot of the developmental stage of the subject teeth (Y-axis) against the calendar age of the subjects (X-axis) showing delayed upper first molar development was fitted to the logistic curve. The tooth development was at the stage of initial cleft formation was delayed 2.5 years or more, according to these curves.View full abstractDownload PDF (998K)
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