It has been widely recognized that the drinking of strongly acid hot spring water often causes gastrointestinal symptoms. However, the research in this subject has been distinctly sporadic. Fundamental and clinical experiments were undertaken to ascertain the effect of the drinking of Tamagawa Spa on the gastrointestinal mucosa. The property of Tamagawa Spa, Akita prefecture, is acid vitriol spring whose pH value is 1.2, and it contains a large amount of hydrochloric acid and hydrogen sulfide. 1. Studies in Man The changes of gastric mucosa in 36 subjects after the drinking of hot spring water were observed by gastrocamera or gastrofiberscope. Varying degrees of hyperaemia were found in all subjects administered non-diluted hot spring water, whereas these change were markedly reduced in subjects administered two times and over diluted hot spring water. No instances of erosion or ulceration were seen in any of the subjects. 2. Experiments in Animals Twenty three healthy rabbits, orally administered non-diluted or diluted hot spring water for seven days were autopsied. The changes of gastric mucosa were similar to those in man, and no significant influence on the jejunal mucosa was seen. Microscopically the affected mucosa revealed degeneration of the epithelial cells of gastric gland and edema of the submucosa. Based on the critical review of former studies, several important factors were suggested to explain the occurrence of gastric impairment after the drinking of Tamagawa Spa water.
Since cholesterol induced atherosclerosis was first produced by Anitchkow in the rabbit, lipid deposits in the arterial wall had generally been admitted as an early change of arteriosclerosis. Recently, Akiya and Misawa suggested that there might be some relation between the development of arteriosclerosis or hypertension and much intake of silicic acid contained excessively in drinking water and grain. Also, it was stated that Arimoto had produced atherosclerosis and coronary arteriosclerosis accompanied with no lipid deposits by the administration of sodium silicate peritoneally in the rabbits. Therefore, the author performed following experiments to clarify how the repeated and excessive administration of silicate could influence upon the development of arteriosclerosis and the disturbance of lipid metabolism in the blood. Twenty male white Leghorn cockerels (2 months old) were divided into following 4 groups and fed by a commercial chick mash (bog/day) and autopsied after 6 weeks of the experiments: A group (control) was fed only with the mash, B group with the mash added cholesterol (1g/day), C group with the mash and sodium silicate (1g/day), D group with the mash and cholesterol (1g/day) plus sodium silicate (1g/day). The aorta divided into ascending, thoracic and abdominal portions and the arterioles of the other organs (heart, kidney, liver, spleen and lung) were investigated histologically. Serum total cholesterol, β-lipoprotein, triglyceride, NEFA and uric acid were measured. And following results were obtained. 1) Although serum total cholesterol and β-lipoprotein changes before and after the experiment among the 4 groups showed no significant difference, it was suggested that the administration of silicate might promote the disturbance of cholesterol and β-lipoprotein metabolism in the blood so that a tendency was shown that these levels in the C group increased more than in the A group and the changes in the D group more than in the B group. But a significant difference was proved only between the A group and the B group or the D group in β-lipoprotein. 2) Triglyceride and NEFA showed no significant difference by the experiment among the 4 groups. 3) The rate of positive Sudan III stain of the ascending aorta was as follows: 0% in the A group, 100% in the B group, 40% in the C group and 40% in the D group. Therefore, the administration of silicate was likely to promote lipid deposits in the ascending aorta. 4) There was a significant difference in serum total cholesterol or β-lipoprotein between the lipid deposits positive and negative groups, but no significant difference in triglyceride or NEFA was proved between the both groups. 5) The percentage of the positive findings of intimal cell proliferation in the thoracic aorta was 20% in the A group, 60% in the B group, 60% in the C group, 40% in the D group. Therefore, it was suggested that silicate promoted the development of thoracic atherosclerosis judged by the intimal cell proliferation and lipid deposits. 6) Diffuse fibrous intimal thickening of the abdominal aorta could be explained by naturally occurring atherosclerosis as the change was observed in the majority of the cases (19 in 20 cases) unrelated to the group classification. 7) Abnormal changes of the arterioles in the other organs were not proved and also no amyloidosis was shown in the kidney. 8) There was no significant difference among the 4 groups in serum uric acid level.
The effects of negative electrostatic potential loading (N. E. P. L) on living bodies, experimentally as well as clinically, have been investigated for about fifteen years. For studies on the effects of N. E. P. L on radiation injuries, two groups of mice (ddN-strain) were used, of which one was exposed to a large amount of x-ray dose in a single exposure method and another was exposed to the same dose in a fractionated exposure method in order to see the relationship between the effects of N. E. P. L and irradiation method. The treated group of the mice was loaded with a potential of -300 Volt, and on the other hand the control group was not electrically loaded. The characteristics of x-rays used in this experiment were as follows: 180KVP, Cu 0.5mm+Al 0.5mm filter, and HVL-1.18mm Cu. The experimental results were as follows; 1. The survival curves for 900γ and 700γ irradiations in a single exposure showed no significant difference between the treated group and the control. 2. For the fractionated exposure in which a dose of 1.000γ was given divided in ten times on every other day, the survivals of the treated group surpassed those of the control before 700γ and after 1, 000γ the survival curve of the treated group gradually approached close to those of the control. 3. By the fractional exposure in which a total dose of 600γ was divided in twenty times, six times per week, the white blood cell count of the both groups decreased to the minimum at about 300γ exposure (about 9, 000 for the treated group and about 5, 000 for the control), and after 600γ exposure, the leucocyte counts of the treated group recovered at about five weeks later to almost the same level as those before irradiation, but on the contrary the control group showed no such recovery. Moreover, concerning the histological findings of the bone marrow in femur and sternum, the spleen and the lymph nodes, it was revealed that the radiation injury of the treated group was less than that of the control, and the recovery of the injury after irradiation in the treated group was better than that of the control. 4. Summarizing the above results, it was concluded that the N. E. P. L treatment was effective for the recovery of radiation injuries by fractionated exposure, but it proved much less effective by single exposure of a large dose. Further investigations would be necessary to clarify the mechanism of the action of the N. E. P. L treatment on radiation injuries.
At Kageyu, Nanasawa, and Kusatsu Spas, the author studied the balneological effects on lipoprotein, triglyceride, non-esterified fatty acids and fatty acid components in total lipids, cholesterol ester fraction, triglyceride fraction, non-esterified fatty acid fraction and phospholipid fraction in sera. The results were as follows.: (1) At Kageyu and Kusatu Spa, a single bath showed a quantitatively significant effect on non-esterified fatty acids but not on β-lipoprotein and triglyceride. An hour later after the bath, non-esterified fatty acids rose at maximum. (2) Successive baths decreased β-lipoprotein at Kageyu, pre β-lipoprotein at Nanasawa but no significant change was observed in non-esterified fatty acids and triglyceride. A week after initiation of daily baths, value of β-lipoprotein and per cent of pre β-lipoprotein were lowered. (3) Successive baths had no significant influence on fatty acids.