The purpose of this study was to show that the pathological changes in the tympanic cavity of the preliminary report were not made by electron beam irradiation. According to the electron iso dose curve, when the pharyngal orifice of the auditory tube was irradiated with the same condition as the prelimimary study, the tympanic cavity would be suffered from the dispersed beam at a dose of less than 200 rad.
We conducted three experiments to know the histopathological effects of electron bean irradiation to the tympanic cavity with dose of 200 rad. Guinea pigs, with intact tympanic membrane and normal Preyer reflex were used.
1) One of the temporal bones of the guinea pig was irradiated with electron beam at a dose of 200 red.
2) One of the temporal bones of the guinea pig was irradiated with electron beam at a dose of 2, 000 rad. Electron iso dose curve showed that the other site of the temporal bones would be irradiated at a dose of less than 200 rad. We observed the pathological findings of the nonirradiated site.
3) Non-irradiated guinea pigs.
1 M, and 2 M after irradiation, animals were sacrificed and serial sections of the middle ear were made. Histopathological findings of the tympanic cavityty were as follows.
1) No remarkable changes were observed in the tympanic cavity.
2) In the irradiated site of the temporal bones, dillatation and proliferation of blood vessels, and slight round cell infiltration were detected within the submucosa. Mucosal epithelium showed no discernible alterations. The tympanic cavity was filled with acidophilic effusion, containing only a few cells, such as lymphocytes, macrophages, and fibrin. Neutrophilic cells were also sparsely found in the effusion. In the non-irradiated site of the temporal bones, histopathological findings were almost normal.
3) The tympanic cavity showed no discernible changes.
Histopathological findings of the auditory tube were as follows.
1) A few inflammatory cell infiltration, and slight proliferation of the fibroblasts within the submucosa were observed, around the pharyngeal orifice. Mucosal epithelium showed no remarkable changes. The lumen of the tube was filled with thick mucoid substance secreted from the tubal glands, containing only a few inflammatory cells. In the group 2, and 3, localization and severity of changes were almost same as in the group 1. Those changes were recognized as the physiological response against an ordinary infection.
In summary, the present study revealed that the electron beam irradiation at a dose of 200 rad to the tympanic cavity caused no discernible alterations. And those findings suggested that in the preliminary report the histopathological changes in the tympanic cavity were induced not by irradiation but by the experimental tubal insufficiency.
抄録全体を表示