An automated biological cell sorting system (A.B.C.S.) which is based on laser light scattering by a single biological cell has been designed and constructed for various clinical applications. The principal part of the system consists of rapid cell flow, forward laser light scattering, signal processing anddisplay, and cell sorting portions.
Using the A. B. C. S. size distributions ofseveral groups of small polymer spheres were measured and then were separated according to their size in order to investigate systematically the optimal operating condition of the system. The particle separation was performed at a rate of 4000/s with a separation efficiency of about 70%.
Various biological cells such as red blood cells and lymphocytes were analyzed and successfully separated employing the A. B. C. S. From the results, several morphological infor-mations about the cells were obtained. One of the results is that human peripheral lymphocyte has a different size distribution from that of human tonsillar lymphocyte.
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