Serum protein fractions of patients with Behcet's syndrome (incomplete type, 3 males and 3 females), recurrent aphthous ulcer (4 males and 2 females), simple aphtha (1 male and 2 females) and erythema exsudativum multiforme (EEM) (1 male and 3 females) were analyzed quantitatively by electrophoresis on cellulose acetate membrane and qualitatively by immuno-electrophoresis on agar gel plate' γ-Globulin and α₂-globulin were incressed in Behcet's syndrome. γ-Globulin was also increased significantly in recurrent aphthous ulcer and EEM.
By immuno-electrophoretic analysis the precipitation lines of haptoglobin (4 in 6 patients), ceruloplasmin (5), transferrin (4), β_1c-globulin (3), IgA (6) and IgG (5) were increased in Behcet's syndrome. In recurrent aphthous ulcer the similar tendency was noticed. In EEM ceruloplasmin, haptoglobin, β_1c-globulin, IgA and IgG were increased. Increase in these fractions suggests that some immunolgic mechanism may be involved in the etiology of the diseases.

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In the routine activities of a clinical examination laboratory, the laboratory technician is faced with difficulties of processing many samples rapidly and economically within a short space of time.
It is generally recognized the invention of an electric floatation method has made the precise and ready fractionation of protein in the blood or serum possible. It has been eagerly welcomed not only in the field of clinical medicine, but also in basic medicine as well as in the realm of protein chemistry. However, in order to fractionate a protein sample by this method it is necessary that a sample should be concentrated to a prescribed degree. This is particularly necessary with low-concentration samples such as the saliva, urine and medullary fluid.
In the present study, the authors comparatively studied the effectiveness between the Carbowax method, one of widely used concentration methods, and the freezing-dry method developed by the authors. The original saliva sample of 0.13g/50ml could be concentrated to 1g/50ml by Carbowax 4000 and to2g/50ml by Carbowax 1500 in two hours respectively. On the other hand, the use of our free-zingdry method could concentrate the same sample to 10g/50ml within the same time length.
From this comparative study, the following conclusions were obtained.
1. As compared with the Carbowax method, the freezing-dry method could process the same specimen at a speed 10 times as that of Carbowax 4000 and 5 times as that of Carbowax 1500. It was possible to obtain a highly-concentrated protein fluid in a very short length of time.
2. The manipulation of the method was easy and there was no possibility of sample undergoing a degenerate change.
3. Influence due to change in salts, which happens as a result of concentrated saliva with the electric floatation method, did not appear with the present method.

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The effect of chronic inflammation on the turnover of the gingival epithelium of the mouse was autoradiographically studied, using the H³-thymidine. Inflammation was artifically induced by applying croton oil to the site.
No differrnce was observed in the migration pattern of the gingival epithelial cell, the cell migration being passive and being almost identical to that in normal. In epithelial attachement, the cell migration was observed from the stratum germinativum toward enamel, margin and/or stratum corneum through the complicated horizontal, vertical and diagonal direction. In downgrowing oral epithelium, migration can be ditected in two ways migration was noted toward the stratum corneum and migration toward the gingival margin gradually approaching the stratum corneum. In oral epithelium, migration was almost in a straight line toward the stratum corneum, while in interdental papilla migration was seemed to be almost identical to that in epithelial attachment.
The radioactive index in the experimental group showed higher value at various site of the gingiva than that in the control group, the differnce being statistically significant especially in 12 hours and 3 days. The maximum value in the control group was observed in 24 hours in oral epithelium and downgrowing oral epithelium in 2 days in epithelial attachment and in 24 hours in interdental papilla, but in the experimental group was observed in 12 hours, 24 hours and 24 hours respectively.
In the experimental group, the turnover time of the gingival epithelium were 3.7 days in epithelial attachment, 3.4 days in downgrowing oral epithelium, 3.3 days in oral epithelium and 4.2 days in interdental papilla, while in the control group they were 5.4 days, 4.6 days, 5.7 days and 4.7 days, respectively showing a considerable, shortning of time at all site.

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This study reported the epidemiological survey of an adult group, as the examined method, to measure periodontal pockets. Fourty-five hundred and seventy six factory workers at electric company in Chiba, were involved in this investigation.
Seven hundred individuaes, (aged from 15 to 49 years) were selected at random on the basis of each five aged frame, and were examined.
The following conclusion can be detected in this investigation.
Rate of shallow periodontal pocket (2mm) in each group decrease gradually from young age group (15 to 19 years old) to all age group (45 to 49 years old).
In the maxillary arch, this observation showes that the postrior regoin had a more deep pocket depth than the anterior region.
In the mandibular arch, there is no significant diferences between the pocket depth of anterior and that of posterior.
There is a suggestion that no significant differences between the pocket depth of men and that of women.

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This study was conducted to determine the effects of insadol^® on the periodontal patients during a seven-week experimental period. The findings reported to support the following conclusion.
1) The effects of insadol^® on the depth of the periodotal pocket, the teeth mobility, that was mesured by using periodonto-mater, and the level of the alveolar bone that was evaluated by using the standardized X-ray photographies, did not differ ststistically significant degree from those of the placebo. (two-tailed t-test).
2) A Significant deference, (P<0.05) was observed between Insadol^® and Placebo, as the result of this, we considered that the influence of minus effect on the improvement of the subjective symptoms.
3) This study could not take the information about the side effect of this drug.
4) This study could not find the significant effect of the Insadol^® in the periodontal therapy during a seven-week administrative period.

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Although there are employed various types of systemic and local treatment for the periodontal diseases. both aftercare and home-care treatment methods are encouragep here as important means for the prevention of a reoccurrence as well as the enhancement of treatment effect.
As a gingival massage agent contoining 0.1% Hinokitiol, “Paradent Ace” is generally used for the purpose of a homecare medicel agent. The present study is concerned with its clinical effects on 2 groups of the patients suffering from different degree of the periodontal diseases as follows.
The group A consisted of 25 male and female subjects of gingivitis simplex, gingivitis gravidarum or hyperplastic gingivitis and 25 subjects of periodontitis marginalia (P₁, P₂, P₃).
Of the total of 50, these were classified as highly effective (5 with 10%), effective (22 with 44%), slightly effective (16 with 32%) and unidentified effectiveness (7 with 14%).
With the group A, the following points were particularly noteworthy.
1. The application of this gingival massage agent was quite effective for cases in which the inflammatory symptoms of bleeding and pus discharge, and halitosis were present.
2. The looseness of teeth, dispppearance of gingival pockets and change in the gingival coloration were improved according to the amelioration of the above-mentioned symptoms.
3. The degree of oral cleanliness was not influenced by this gingival massage agent. With the cases in which oral cleanliness was improved, causes should be sought in the proper use of tooth-brush and mechanical stimulation exerted by the fingertip when applying massage to the gingiva.
The group B comprising 10 male and femall subjects of gingivitis simplex and hyperplastic gingivitis and 16 subjects of peniopontal marginelis (P₁, P₂, P₃) were used for the comparison of Munsell color indices before anp after the application of “Paradent Ace” agent.
The following finings were obtained from this comparative examination.
1. With the cases of gingivitis, the hue was improved around the center of 5 R while the value was improved around 5.5. Although the distribution range was improved respectively, no clearly established change was recognized in the chroma.
2. With the cases of periodontitis marginalis, on the other hand, the hue moved from the center of R in the direction of 4 R and showed a wider range of distribution. There was no appreciable change in the value and chroma.
In other words, the hue was only improved with the cases of periodontitis marginalis.
On the strength of findings from the groups A and B summarized above, it is to be concluded that the present giugival massage agent, “Paradent Ace”, is clinical effective on the ground that it acts eneficially to prevent the incidence and reoccurrence of periodontal diseases and promotes their treatment.

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The treatment process of a female periodontal patient, 33 of age, with open bite caused by tongue thrusting habit was reported. By tight cooperation between periodontists and orthodontists and with enough care, the treatment was proceeded for 2 years and 4 months, and completed with success. One year and 4 months follow up showed no clinical sign of relapsing. The significancy of ortho-periodontal relationship were discussed.

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