The goal of current periodontal therapy is to provide connective tissue adhesion on root surfaces that have lost attachment for which the adhesive capacity of periodontal ligament fibroblasts on the root surface is considered important.
In this study, we investigated the effects of changes in the environments surrounding the cells, including properties of the root surface or matrix, on the mode of adhesion of periodontal ligament fibroblasts and on the expression of integrins, with the aim of identifying the mechanism of cell adhesion in the process of wound healing in periodontal treatment.
Human periodontal ligament fibroblasts (HPLF) were cultured onto collagen-coated glass plates, which imitated the root surface after root treatment. We then examined by immunohistochemistry the effects of the presence or absence of the ligand-binding site (RGD sequence) of fibronectin recognized by integrins on cellular adhesion, as well as the expression of fibronectin, and integrin β
1 and α
2 subunits (integrin β
1 andα
2, respectively), using a confocal laser scanning microscope.
The results showed that fibronectin was expressed on cell surfaces, and integrin β
1 was mainly involved in cell adhesion in the RGDS (-) medium.
On the other hand, although fibronectin and integrin β
1 were expressed on cell surfaces in the RGDS (+) medium, they were minimally involved in cell-substrate adhesion and compensatory expression of integrinα
2, a collagen receptor, was seen.
These findings suggested that characteristics of the root surface and/or the conditions surrounding cells have to be considered in order to be certain of obtaining HPLF attachment during periodontal regeneration. J. Jpn. Soc. Periodontol., 41: 108-117, 1999.
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