Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
Online ISSN : 1880-408X
Print ISSN : 0385-0110
ISSN-L : 0385-0110
Volume 48, Issue 4
Displaying 1-8 of 8 articles from this issue
A Foreword
Original Work
  • Ryousuke Ishizuka, Hirofumi Miyaji, Tsutomu Sugaya, Masamitsu Kawanami
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 255-266
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    We evaluates periodontal regeneration in a dehiscence model after the application of BMP-2 solution and collagen hydrogel to the root dentin surface.
    Materials were 128 premolars of 8 beagles. After a partial thickness muco-gingival flap on the buccal site was elevated, periodontal dehiscence defects with a length of 6 mm were surgically produced. Root surfaces were scaled and planed to remove periodontal ligament and cementum.
    Denuded dentin surfaces were subsequently demineralized with 24% EDTA and divided into four groups. In the control group, neither BMP-2 solution nor collagen hydrogel was applied. In the Gel group, collagen hydrogel was applied to root dentin surfaces. In the BMP group, BMP-2 (1000 μg/ml) solution was applied to root dentin surfaces. In the BMP-Gel group, BMP-2 (1000 μg/ml) solution and collagen hydrogel were applied to root dentin surfaces. Histological analysis was conducted 1, 2, 4 and 8 weeks after surgery. Histometrical analysis was conducted 4 and 8 weeks after surgery.
    At 8 weeks, new bone formation in the BMP-Gel group was significantly greater than in any other group. New cementum formation in the BMP-Gel group was significantly greater than in the control group. Ankylosis in the BMP-Gel group was significantly greater than in the control group. Down-growth of the junctional epithelium in the BMP-Gel group was significantly smaller than in the control group.
    These results suggest that the application of BMP-2 solution and collagen hydrogel to root dentin surfaces is conducive to periodontal tissue regeneration. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 255-266, 2006.
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  • Masaharu Sawaguchi
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 267-275
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    As part of our investigations into the basic usage of hand scalers, we removed dental calculus using sickle-type and curette-type scalers, and measured the amount of force applied to teeth for removal of dental calculus in three planes (horizontal, lateral and vertical forces). Based on the results, the differences between experienced and novice groups were determined. Subjects were six individuals with at least four years of clinical experience and six individuals with no experience in periodontal care. In the present study, standardized models with artificial calculus were used. The horizontal force and total force (sum of horizontal, lateral and vertical forces) in the novice groups was significantly greater than in the experienced groups with both sickle-type and curette-type scalers. In addition, with both sickle-type and curette-type scalers, there were no significant differences in lateral force, vertical force or tooth surface force (sum of lateral and vertical forces) between the experienced and novice groups.Furthermore, with the curette-type scaler, for both the experienced and novice groups, a significant correlation was seen between the horizontal and vertical forces, and between the horizontal and tooth surface forces: the greater the horizontal force, the greater the vertical force or parallel force. The above findings suggest that, for both sickle-type and curette-type scalers, horizontal force affects the removal of dental calculus more than the other two forces, and that for efficient removal of dental calculus, it is necessary to develop removal techniques and instruments that minimize horizontal force. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 267-275, 2006.
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  • Masayuki Yoshikawa, Akira Ogata
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 276-284
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    We previously reported the presence of type I, and III collagen (Col-I, III) and proliferating cells in human alveolar bone cells (HABCs) three-dimensional (3D) grafts (spheroid). The existence and differentiation of osteoblasts in the HABC spheroid remains to be clarified, and the function of the HABCs spheroid is poorly understood. We studied the HABCs spheroid on different cultivation days in histological and immunohistochemical staining, using the monoclonal antibody of the expression of Ki-67, Col-I, osteopontin (OPN), and osteocalcin (OC). HABCs differentiation was assessed by alkaline phosphatase (ALP) staining. The number of Ki-67 antigen-positive nuclei was observed in days 1, 3, 7, and 21. During the 21-day study,the positive Col-I reaction of increased and the positive ALP reaction decreased time-dependently. Positive staining with OPN and OC were observed only at day 21 after spheroid formation. These results indicate the entity and differentiation of the osteoblast in the HABCs spheroid and the possibility of mineralization in vitro. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 276-284, 2006.
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  • Tetsunari Hongo, Hirofumi Miyaji, Tsutomu Sugaya, Masamitsu Kawanami
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 285-296
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    We studied the effects of bisphosphonate administration on cementum-like tissue formation and dentin resorption induced by BMP-2 in rats.
    Incadronate (1μg/kg/day) was administered intraperitoneally to rats three times per week, from two weeks before implanting dentin blocks to the end of the observation period. Saline was administered under the same protocol without incadronate to the control group. We prepared 180 dentin blocks from rat teeth roots and treated them with 24%EDTA. Each block had 0,100 or 400μg/ml rhBMP-2 applied. Blocks were then implanted into palatal connective tissue of rats, and specimens were prepared 2, 4, and 8 weeks after implantation for histological and histomorphometric examination.
    Results showed that dentin resorption in the control group was significantly greater than that in the experimental group (p<0.01). New cementum-like tissue formed in the experimental group was also significantly less than that in the control group (p<0.01). 80% of new cementum-like tissue were formed on resorption site of dentin and 20% on the nonresorption site. TRAP positive cell numbers did not show any significant difference between groups.
    These findings suggested that osteoclast-like cells mediated new cementum-like tissue formation, and dentin resorption was suppressed by bisphosphonate administration and osteoclasts this play an important role in has been new cementum-like tissue formation on dentin surfaces to which BMP-2 applied. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 285-296, 2006.
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  • Akihiro Yashima, Kazuhiro Gomi, Junichi Sato, Nobuko Maeda, Takashi Ar ...
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 297-306
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    Photocatalysis of titanium oxide is known to have an antibacterial effect on Pseudomonas aeruginosa and Escherichia coli, and is widely-applied to medical science. Also in dentistry, the use of titanium oxide in dental materials is thought to prevent dental caries and periodontal disease.
    The aim of this study was to examine the antibacterial activity of a titanium oxide coat on the surface of gold-silver-palladium alloy and a commonly used dental metal. Antimicrobial activity was evaluated by the size of each inhibition ring formed on agar and the number of persistent Porphyromonas gingivalis and Streptococcus mutans, after the floating bacteria were directly inoculated on the alloy.
    As a result, inhibitory zones were found in the experimental group coated with titanium oxide, but the inhibitory zones were not found in the shading group covered with aluminum foil or in the non-coated group. Further, in the experiments where bacteria were dropped directly on the discs, exposed to light and inoculated on agar, the number of persistent bacteria decreased with exposure time. On the other hand, the number of bacteria did not change in the shading or non-coating groups, regardless of exposure time. These results suggested that the titanium oxide coated on the gold-silver-palladium alloy surface has an antibacterial effect on Porphyromonas gingivalis and Streptococcus mutans.
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  • Kotaro Matsui, Mohei Yuasa, Takumasa Yoshida, Takahisa Fukuda, Gaku Ak ...
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 307-314
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    We observed the clinical status of periodontal tissues after periodontal flap surgery combined with Nd:YAG laser irradiation based on clinical parameters.
    Materials were 52 teeth in 10 patients, Surgical procedure was as follows: the gingival flap was elevated and root planing and bone defect debridement were done. In the experimental group (Exp) of 26 teeth in 10 patients, Nd:YAG laser was irradiated to the root surface, the bone defect, and the inside of the gingival flap after root planing and bone defect debridement (3.0 w, 30 seconds, 3 times). the laser was also irradiated around the mucogingival junction immediately after surgery (1.5 w, 1 minute, 3 times). In the control group (Cont) of 26 teeth in 10 patients, the Nd:YAG laser was not irradiated combined with surgery.
    Clinical parameters were Probing depth (PD), plaque index (PLI), gingival index (GI), and bleeding on probing (BOP). Clinical and radiographic examinations were done before surgery and 3 and 6 months after. Clinical parameters were subjected to the Wilcoxon signed-rank test.
    No statistical by significant differences were seen between groups for clinical parameters after periodontal surgeriy and abnormal clinical signs such as gingival and bone necrosis and bone loss.
    Our findings indicate that after flap surgery combined with Nd:YAG laser irradiation, periodontal tissues healed uneventfully, enabling us to reduce probing depth and remove gingival inflammation. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 307-314, 2006.
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  • Takashi Matsuoka, Naoyuki Sugano, Satoko Takigawa, Masatoshi Takane, N ...
    Article type: Original Work
    2006 Volume 48 Issue 4 Pages 315-324
    Published: 2006
    Released on J-STAGE: April 12, 2007
    JOURNAL FREE ACCESS
    To determine the efficacy of probiotics on periodontal diseases, Lactobacillus salivarius TI2711 (LS1) was orally administered to volunteers. The 87 subjects were divided into two administration (n=49) and one placebo (n=38) group. Administration groups took either 2x108 (n=39) a day for 12 weeks. At baseline, 4 and 12 weeks after LS1 administration and 4 weeks after the termination of LS1 administration (at week 16), subjects were clinical by examied for probing depth (PD), bleeding on probing (BOP), and plaque control record (PCR), and, subgingival plaque samples were collected from all subjects to measure the number of Porphyromonas gingivalis and L. salivarius bacteria in plaque using real-time PCR. A significant reduction in the number of P. gingivalis bacteria was found in the administration group from 1.12x105 to 2.97x104 at 12 weeks(P<0.05), but, not at week 16 compared to baseline. The number of L. salivarius bacteria increased at 4 and 12 weeks and decreased at week 16. No significant change in the number of L. salivarius bacteria was observed in the placebo group. These results imply a reverse correlation between the numbers of P. gingivalis and LS1. This means that LS1 translocates to subgingival plaque, then decreased the number of P. gingivalis. This data also suggests that LS1 is an effective probiotic. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 315-324, 2006.
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