Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology)
Online ISSN : 1880-408X
Print ISSN : 0385-0110
ISSN-L : 0385-0110
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Volume 54, Issue 2
Displaying 1-9 of 9 articles from this issue
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Mini Review
Original Work
  • Ultrastructural changes of cell spheroids of human alveolar bone periosteal cells during cell culture
    Kazuhiro Tago, Yukiko Koke, Kazuyoshi Higashi, Norikazu Munemasa, Kenj ...
    2012 Volume 54 Issue 2 Pages 155-166
    Published: June 28, 2012
    Released on J-STAGE: April 24, 2013
    DOIhttps://doi.org/10.2329/perio.54.155
    JOURNAL FREE ACCESS
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    We previously reported that spheroids containing three-dimensional proliferative cells may be useful for periodontal tissue regeneration. The aim of this study was to observe the changes of human alveolar bone periosteal cells (HABPCs) in spheroids using ultrastructural and immunohistochemical techniques. HABPCs were suspended at a cell density of 1.8 X 104 cells/cm2 in a 100-mm culture dish. After 10 daysʼ culture, the cells formed spheroids, which were digested by 0.25% trypsin, and centrifuged at 300 g for 3 min. Furthermore, the spheroids were matured for 1, 3, 7 and 14 days by immersion in a 24-well culture dish. The spheroids were examined at 1 day after formation by transmission electron microscopy, which revealed that the HABPCs were scattered in the whole spheroid. Specimens of spheroids were divided into three zones by the cell localization, namely, surface, middle and central zones, at 3 days. The number of cells in the central zone was decreased on day 7.In the middle zone on day 14 cells were found at a low density, with numerous collagen fibers observed in the wide intercellular spaces. When the spheroids were cultured for a prolonged period , the collagen synthesis started in the central zone of the spheroids, and gradually moved to the surface zone of the spheroids. An increase in the number of dense granules in the cells was observed. Furthermore, a similar degree of increase of both Osteopontin (OP)- and Osteocalcin (OC)- immunoreactive cells was noted. The results suggested that the cells in the spheroids had the potential for differentiation direction in the spheroids from central to surface zone. And the possibility of bone regeneration following transplantation of HABPC spheroids based on their ability to express OP and OC in alveolar bone. Nihon Shishubyo Gakkai Kaishi(J Jpn Soc Periodontol)54(2):155-166,2012.
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  • Topical injection of osteoprotegerin prevents alveolar bone loss in rat experimental periodontitis
    Chie Mihara-Wada, Kaku Tokunaga, Hiroyuki Seto, Eijiro Sakamoto, Yuka ...
    2012 Volume 54 Issue 2 Pages 167-174
    Published: June 28, 2012
    Released on J-STAGE: April 24, 2013
    DOIhttps://doi.org/10.2329/perio.54.167
    JOURNAL FREE ACCESS
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    Osteoprotegerin (OPG) is a decoy receptor for Receptor Activator of NF-кВ Ligand (RANKL) and blocks differentiation and activation of osteoclasts. Clinical trials of OPG administration have been successfully performed for bone-destructive diseases such as osteoporosis, bone metastatic cancer, and rheumatoid arthritis. In the case of periodontitis, systemic administration of OPG has been reported to be effective for decreasing alveolar bone loss in rat models of experimental periodontitis. In this study, we investigated the effect of topical OPG injection every other day in a rat model of experimental periodontitis, which was established by a ligature placement at the upper 2 ndmolar. Micro-CT analysis revealed that the distance from the cement-enamel junction to the alveolar bone crest (CEJ-ABC) was higher in the ligature group, and that the CEJ-ABC was significantly shorter in the ligature-OPG group than in the ligature-control group on days 5 and 14 after ligaturing. Analysis of tartrate-resistant acid phosphatase (TRAP) staining revealed that the number of TRAP-positive cells (osteoclasts) was significantly lower in the ligature-OPG group than in the ligature-control group, having decreased to one-third by day 5. These results demonstrate that topical injection of OPG could prevent alveolar bone loss in rat experimental periodontitis, suggesting that OPG may be useful for alveolar bone preservation in periodontitis. Nihon Shishubyo Gakkai Kaishi(J Jpn Soc Periodontol)54(2):167-174, 2012.
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Case Report Review
Case Report
  • A Case Report of moderate periodontitis with chronic desquamative gingivitis
    Hiroko Takeuchi-Igarashi, Hiroshi Ito, Yukihiro Numabe
    2012 Volume 54 Issue 2 Pages 183-192
    Published: June 28, 2012
    Released on J-STAGE: April 24, 2013
    DOIhttps://doi.org/10.2329/perio.54.183
    JOURNAL FREE ACCESS
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    Desquamative gingivitis is a type of gingival keratinization disorder, characterized by chronic ulceration, desquamative erosions and edematous erythema of the free and attached gingiva. Desquamative gingivitis has been known to be refractory to treatment and only a few cases have been reported. Since the cause of this condition is still unclear, symptomatic therapy is the mainstay of treatment. Here, we present the case of a patient who had moderate periodontitis with chronic desquamative gingivitis. He also had poor oral hygiene due to intense pain. Terra- Cortrill® ointment and/or Dexaltin® ointment applied together along with the initial periodontal preparation improved oral hygiene and resulted in a good outcome without periodontal surgery. The patient has shown a satisfactory clinical course with long-term by supportive periodontal therapy (SPT). Nihon Shishubyo Gakkai Kaishi(J Jpn Soc Periodontol)54(2):183-192, 2012.
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  • 15-year Clinical Course of a Patient with Generalized Aggressive Periodontitis Followed with Regular examination of the serum titers of IgG antibodies against periodontal bacteria
    Kazuya Tomikawa, Yoshihiro Iwamoto, Hyogo Ohe, Hideo Arai, Tadashi Yam ...
    2012 Volume 54 Issue 2 Pages 193-202
    Published: March 28, 2012
    Released on J-STAGE: April 24, 2013
    DOIhttps://doi.org/10.2329/perio.54.193
    JOURNAL FREE ACCESS
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    For the treatment of patients with aggressive periodontitis, a thorough control of the infection is required to reduce the disease activity. Detection of periodontal pathogens by a polymerase chain reaction-based method or measurement of the serum IgG antibody titers by enzyme-linked immunosorbent assay are now widely used for microbiological diagnosis and assessment of the disease activity. We report the case of a 25-year-old woman with impaired neutrophil phagocytosis ability who suffered from generalized aggressive periodontitis . After the initial periodontal treatment and periodontal regenerative surgery, she received the final prosthodontics, and supportive periodontal treatment (SPT) was initiated. For over a decade, from the beginning of the treatments, the clinical parameters and serum titers of IgG periodontal pathogens have been monitored, and the relationships between the twoanalyzed. During the SPT, recurrence and progression of periodontitis was seen around site 26, despite maintenance of a stable periodontal condition at other sites. The serum IgG antibody titers against periodontal pathogens were elevated in parallel with the progression of periodontitis at site 26. In the present case of generalized aggressive periodontitis, the serum IgG antibody titers reflected the disease activity of periodontitis even around a single tooth, suggesting the usefulness of the examination for assessment of the disease activity. Nihon Shishubyo Gakkai Kaishi(J Jpn Soc Periodontol)54(2):193-202, 2012
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Dental Hygienist Corner
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