Reducing sugars were detected, using copper bis (bipyridine) complex (CBB) as a mediator, of which redox reaction with the reducing sugars offers the following scheme of detection:
In solution; CBB
2++reducing sugar→CBB
++aldonic acid
At electrode; CBB
+-
e-→CBB
2+CBB reacts specifically with reducing sugars. The content of the reducing sugar can be detected from the current observed on the anodic oxidation of CBB
+. The amounts of reducing sugars detected in IAG gelatins are different from one another, but in general, the acid-psocessed gelatin includes a larger amount of reducing sugars than the alkali-processed.
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