Proceedings of the Japan Academy, Series B

Chromosomal Studies on Interspecific Hybrids of Butterflies (Papilionidae, Lepidoptera). X

Crosses among P. machaon britannicus, P. machaon gorganus, P. machaon hippocrates, and P. zelicaon

Kodo MAEKI, Shigeru A. AE

1977 Volume 53 Issue 4 Pages 133-137
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.133

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A Cytological Evidence for Gynogenetic Development of the Ginbuna (Carassius auratus langsdorfii)

Yoshio OJIMA, Norihide ASANO

1977 Volume 53 Issue 4 Pages 138-142
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.138

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A Simple Giemsa Method for the Differential Staining of Sister Chromatids with a Note on the Presumptive Mechanism Involved

Shunichi SAKANISHI, Susumu TAKAYAMA

1977 Volume 53 Issue 4 Pages 143-146
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.143

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Chinese hamster strain cells were grown in the presence of BrdU (10μM final concentration) for 26hr. The cells were ordinarily air-dried, and the slides were incubated in Na₂HPO₄ (1.0M) at 75°C for 2 minutes. After Giemsa staining, thee treated chromosomes showed a clear differential staining between their sister chromatids. Results of the examination by the use of fluorescent Feulgen reaction suggested that the chromatid which stained weakly with Giemsa contains less DNA than the chromatid which stained darkly.

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A Comparative Study of Chromosomes in Seven Species of Lemurs, with Special Reference to Homology of G-banding Patterns

Ei-ichi TAKAHASHI, Norio KONDO

1977 Volume 53 Issue 4 Pages 147-151
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.147

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G-banded karyotypes are compared in Lemur mongoz, L. f. fulvus, L. catta, L. macaco, L. variegatus, Hapalemur griseus and Lepilemur mustelinus. The occurrence of remarkable homology of chromosome- and arm-pairs in the Lemur species suggests their close relationship. H. griseus is not far divergent from the Lemur species. L. mustelinus is of a distant relative to Lemur and Hapalemur.

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The Transforming DNA Sequence Common to Highly Oncogenic Human Adenoviruses, Type 12, 18 and 31

Kei FUJINAGA, Setsuko OJIMA, Shoki YANO, Morikazu SHINAGAWA

1977 Volume 53 Issue 4 Pages 152-155
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.152

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DNA-DNA hybridization experiments have shown that most, if not all, of the nucleotide sequences of the Ad 12 transforming segment, EndoR•HindIII-G fragment at the left 7.2% of Ad 12 DNA molecule, are also present in viral DNAs of Ad 18 and Ad 31, other members in human adenovirus subgroup A, and are not detectably present in viral DNAs of Ad 7 (Subgroup B) or of Ad 2 and 5 (Subgroup C).

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The Binding of Rat Luteinizing Hormone (rLH) to Slices of Luteinized Ovaries and Intrasplenic Ovarian Grafts

Takako SAIGA-NARUMI, Seiichiro KAWASHIMA

1977 Volume 53 Issue 4 Pages 156-160
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.156

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The specific binding of ¹³¹I-rLH to slices of intrasplenic ovarian grafts (heterogeneous ovarian tumors) was less than that to slices of luteinized young rat ovaries or of intact normal adult ovaries. Uptake by slices of luteinized old rat ovaries of comparable age was also greater than that by the intrasplenic ovarian grafts, although the grafts accumulated significantly larger amounts of rLH than did liver, kidney or muscles (diaphragm).

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Immunochemical Studies on Human Cancer. I

Cancer-Associated Antigen Reacting with Anti-Stomach Cancer Immune Serum

Ken FURUKAWA, Hisao TAKIZAWA, Shoei ISEKI

1977 Volume 53 Issue 4 Pages 161-166
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.161

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Rabbit antiserum immunized with water-soluble extract of stomach cancer and absorbed with normal human blood plasma and stomach mucosa extract contained a precipitin which reacted not only with the stomach cancer but also with colon cancer extracts. The precipitation reaction was inhibited by both D-glucosamine and D-galactosamine. The absorbed specific antiserum reacted with colon cancer extracts, but not with embryonic tissue extracts. These findings demonstrate an antigenic difference between carcinoembryonic antigen (CEA) and the antigen found in gastric cancers in this study.
The antigenic material eluted after polyacrylamide gel electrophoresis showed a single protein band on disc-electrophoresis and no color developed with alcian blue or formazan staining for glycoprotein, indicating that the antigen found in gastric cancers in this study consists principally of protein. The antigenic substance in the cancer tissue may be excreted into the urine in cancer patients.

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Immunochemical Studies on Human Cancer. II

Detection of a Urinary Antigenic Substance Common to Gastric Cancer Tissue

Ken FURUKAWA, Shoei ISEKI

1977 Volume 53 Issue 4 Pages 167-171
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.167

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Antibody reacting with urine from cancer patients was produced in rabbits immunized with urine from gastric cancer patients. Specimens of intact urine from the cancer patients revealed a direct precipitation reaction with the specific antiserum by the agar gel diffusion technique. The reaction of the specific antiserum with urine from cancer patients was inhibited by most concentrated urine specimens from the cancer patients. The reaction was also inhibited by D-glucosamine hydrochloride and D-galactosamine hydrochloride, i.e., by the same sugars as in the case of the reaction of anti-gastric cancer immune serum.

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Isoelectric Analysis of B Gene-Associated α-Galactosyltransferases in Human Serum and Saliva

Koichiro KISHI, Hisao TAKIZAWA, Shoei ISEKI

1977 Volume 53 Issue 4 Pages 172-177
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.172

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Two types of α-galactosyltransferase with distinct isoelectric points were detected in serum from blood group B and group AB donors. One enzyme focusing at about pH 4.8 (4.4-5.6) had a reactively low enzymatic activity, whereas the other focusing at about pH 8.8 (7.2-9.8) had a higher enzymatic activity. On the other hand, all saliva samples from the same donors yielded a single peak with corresponding transferase activity, focusing at about pH 8.1 (6.9-9.0).

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X-ray Diffraction of Active Frog Skeletal Muscle Before and After a Slow Stretch

Haruo SUGI, Yoshiyuki AMEMIYA, Hiroo HASHIZUME

1977 Volume 53 Issue 4 Pages 178-182
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.178

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Etude Quantitative de la Cytoarchitecture de la Moelle Epinière chez le Chat et le Poulet

Akira KANEMITSU

1977 Volume 53 Issue 4 Pages 183-188
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.183

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Etude Quantitative des Neurones dans la Moelle Cervicale chez un Cas de l'Hémisphérectomie Cérébrale

Akira KANEMITSU, Fusahiro IKUTA

1977 Volume 53 Issue 4 Pages 189-193
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.189

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Mutagenicity of Flavone Derivatives

Takashi SUGIMURA, Minako NAGAO, Taijiro MATSUSHIMA, Takie YAHAGI, Yuko ...

1977 Volume 53 Issue 4 Pages 194-197
Published: 1977
Released on J-STAGE: October 06, 2006

DOIhttps://doi.org/10.2183/pjab.53.194

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