Chinese hamster strain cells were grown in the presence of BrdU (10μM final concentration) for 26hr. The cells were ordinarily air-dried, and the slides were incubated in Na2HPO4 (1.0M) at 75°C for 2 minutes. After Giemsa staining, thee treated chromosomes showed a clear differential staining between their sister chromatids. Results of the examination by the use of fluorescent Feulgen reaction suggested that the chromatid which stained weakly with Giemsa contains less DNA than the chromatid which stained darkly.
G-banded karyotypes are compared in Lemur mongoz, L. f. fulvus, L. catta, L. macaco, L. variegatus, Hapalemur griseus and Lepilemur mustelinus. The occurrence of remarkable homology of chromosome- and arm-pairs in the Lemur species suggests their close relationship. H. griseus is not far divergent from the Lemur species. L. mustelinus is of a distant relative to Lemur and Hapalemur.
DNA-DNA hybridization experiments have shown that most, if not all, of the nucleotide sequences of the Ad 12 transforming segment, EndoR•HindIII-G fragment at the left 7.2% of Ad 12 DNA molecule, are also present in viral DNAs of Ad 18 and Ad 31, other members in human adenovirus subgroup A, and are not detectably present in viral DNAs of Ad 7 (Subgroup B) or of Ad 2 and 5 (Subgroup C).
The specific binding of 131I-rLH to slices of intrasplenic ovarian grafts (heterogeneous ovarian tumors) was less than that to slices of luteinized young rat ovaries or of intact normal adult ovaries. Uptake by slices of luteinized old rat ovaries of comparable age was also greater than that by the intrasplenic ovarian grafts, although the grafts accumulated significantly larger amounts of rLH than did liver, kidney or muscles (diaphragm).
Rabbit antiserum immunized with water-soluble extract of stomach cancer and absorbed with normal human blood plasma and stomach mucosa extract contained a precipitin which reacted not only with the stomach cancer but also with colon cancer extracts. The precipitation reaction was inhibited by both D-glucosamine and D-galactosamine. The absorbed specific antiserum reacted with colon cancer extracts, but not with embryonic tissue extracts. These findings demonstrate an antigenic difference between carcinoembryonic antigen (CEA) and the antigen found in gastric cancers in this study. The antigenic material eluted after polyacrylamide gel electrophoresis showed a single protein band on disc-electrophoresis and no color developed with alcian blue or formazan staining for glycoprotein, indicating that the antigen found in gastric cancers in this study consists principally of protein. The antigenic substance in the cancer tissue may be excreted into the urine in cancer patients.
Antibody reacting with urine from cancer patients was produced in rabbits immunized with urine from gastric cancer patients. Specimens of intact urine from the cancer patients revealed a direct precipitation reaction with the specific antiserum by the agar gel diffusion technique. The reaction of the specific antiserum with urine from cancer patients was inhibited by most concentrated urine specimens from the cancer patients. The reaction was also inhibited by D-glucosamine hydrochloride and D-galactosamine hydrochloride, i.e., by the same sugars as in the case of the reaction of anti-gastric cancer immune serum.
Two types of α-galactosyltransferase with distinct isoelectric points were detected in serum from blood group B and group AB donors. One enzyme focusing at about pH 4.8 (4.4-5.6) had a reactively low enzymatic activity, whereas the other focusing at about pH 8.8 (7.2-9.8) had a higher enzymatic activity. On the other hand, all saliva samples from the same donors yielded a single peak with corresponding transferase activity, focusing at about pH 8.1 (6.9-9.0).