The H-active heptasaccharide showed nearly the same chromatographic mobility with lacto-
N-
neotetraose after the liberation of fucosyl, galactosyl and
N-acetylglucosaminyl residues by the enzyme actions. The A-active octasaccharide liberated
N-acetyl-galactosamine by the enzyme from pig liver, thus abolishing its A activity and instead manifesting H activity. After the liberation of
N-acetylgalactosaminyl residue, it gave the same R
LNT with that of H-active heptasaccharide. The B-active octasaccharide lost B activity through the liberation of galactose and manifested H activity by α-(1→3)-D-galactosidase from
Cl. Sporogenes Maebashi. After the liberation of galactosyl residue, it gave the same R
LNT with that of H-active heptasaccharide.
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