Attempts were made to expect continuous growth of normal human cervical epithelial cells in order to study possible oncogenic effects of Herpes Simplex Virus type 2 (HSV-2). Surgical specimens from patients undergoing hysterectomy and other operation for non-malignant pathology were obtained and cultured with a “sandwich method.” The life span of normal endo- and ectocervical cells was limited only to 2 weeks. The monolayer cells treated with both Feeder layer and Epidermal Growth Factor (EGF) showed longer life for several months. The growing cells under the above condition infected with HSV-2 showed less degree of cornification and survived in soft agar. The normal human cervical epithelial cells required both Feeder layer and EGF, but no oncogenic transformation was obtained with HSV-2 infection.
Extracted chromatin from wheat germ and 3 days-germinated seedlings is negatively stained with sodium silicotungstate, and examined by means of electron microscopy. The chromatin consists of fibrous elements, and is about 200Å in diameter. The germ chromatin appears to be made up of a number of aggregates which have a mean diameter of 2, 200Å. In contrast, no aggregates are present in the seedling chromatin.