The changes of vascular architecture in the endometrium on the 5th day after coitus were studied in the rabbit by means of scanning electron microscopy of the resin casts, in relation to implantation. The changes of the endometrial vascualr bed in the placental fold were remarkable. Vessels in the cores of the placental fold became voluminous and elongated. The subepithelial plexus surrounding uterine glands was found well developed. There was neither evidence for extravasation of blood nor for local vascular differences indicating implantation sites in the endometrial vascular bed.
A female rat with chromosome mosaic consisting of two cell types (+/t) and (i/t) was found in offspring from the Lewis strain rat with the 1/12 translocation. Two cell types (+/t and i/t) occurred at nearly equal frequency in the tail and ear tissues, though the former was less than the latter in marrow cells. The chromosomes with translocation and inversion was independently transmitted to the offspring at the ratio as expected.
The chromosomes of 30 species of the Labridae (Pisces) involving 3 species of the Bodianinae, 22 species of the Corinae and 5 species of the Cheilininae were comparatively studied. The chromosomal changes were remarked in relation to the evolutional relationship. Detailed accounts will be presented elsewhere in the near future.
The frequencies of chromosome aberrations and sister chromatid exchanges (SCEs) were investigated in cultured human and Chinese hamster cells under the exposure to synthetic amino-γ-carboline derivatives, Trp-P-1 and Trp-P-2, without application of any exogenous metabolic activation system. Both compounds were found to induce chromosome aberrations and SCEs in PHA-stimulated human lymphocytes, Chinese hamster Don-6 and B-131 cells. In the human fibroblastic cell line, however, Trp-P-1 only induced SCEs, while Trp-P-2 induced neither aberrations nor SCEs.
Morphological characteristics of metaphase chromosomes were analyzed in the BN strain rat following the Q-, C- and Ag-staining procedures. The BN rat had an unusually long Y chromosome corresponding in length to no. 10 autosomes. Certain polymorphic markers of the rat chromosomes defined by Sasaki et al. (1979) were remarkable for the short arm of chromosomes 3, 12 and X as well as for C-bands of chromosomes 4, 5, 7 and 9. Ag-NORs of different size were found located on the short arm of chromosomes 3, 11 and 12.
Cyto-histological and biochemical studies on pollen abortion in Datura alba plants treated with some gametocides were undertaken. Histological studies revealed that the abortion of pollen in treated plants was associated with the abnormal tapetal enlargement due to starvation caused by poor vascular differentiation. Cytological observations showed minor meiotic abnormality of less significance in the anthers of complete male-sterile plants. Histochemical and biochemical findings corroborated the histological observations as there was a deficiency of carbohydrates, proteins, DNA, histones, enzyme acid phosphatase and free proline in the anthers of complete male sterile plants. It is noted that the mechanism of male sterility induced by gametocides seems to correspond to those of cytoplasmic and genie male sterility which were found in many kinds of plant species.
Incidence of hyperplastic adrenocortical nodules was smaller in neonatally estrogenized mice than in the controls after ovariectomy performed at 40 days of age. The difference may be accounted for by lower serum levels of LH and FSH in the neonatally estrogenized, ovariectomized mice than in the ovariectomized controls.
Paternity testing was performed in a criminal case on the request of the police. Beside tests for erythrocyte and serum groups, the HLA groups of the family members burnt to death were tested applying the microcytotoxicity inhibition test with the cadavers' sera as a new attempt. Test results of the Rh, the Km, and the HLA-A systems excluded the paternity of the father to the first child, who was presumed to by an illegitimate of an acquaintance of the mother.