Banding growth in liquid media exhibited by mutants in orthophosphate repressible cyclic phosphodiesterase,
cpd-1 and
cpd-2, was found to be due to the alternate appearance of submerged mycelia and submerged conidia. Thus
cpd-1 and
cpd-2 exhibited rhythmic conidiation both in liquid and on solid media, which indicated the persistence of circadian rhythm in liquid media. The examination of the levels of cyclic 3', 5'-AMP in wild type,
cpd-1,
cpd-2 and band (
bd) strain revealed rhythmic oscillations in cyclic 3', 5'-AMP levels with period lengths of about 24hr. Conidiation on solid media took place 2-3hr after the appearance of a major peak of cyclic 3', 5'-AMP.
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