We have developed a novel procedure to analyze highly repetitive and transcribable (Hirt) sequences present in animal cells, that is, total DNA transcription
in vitro. Total DNAs from various animals were transcribed
in vitro in a HeLa cell extract and it was found that one to several discrete RNAs were transcribed by RNA polymerase III. We determined the highly repetitive and transcribable sequences in calf, tortoise, newt and salmon detected by total DNA transcription and demonstrated that 5' parts of these four Hirt sequences have close resemblance to specific tRNA genes. In the cases of tortoise (
Geoclemys reevessi) and newt (
Cynops pyrrhogaster), the 5' parts of these sequences appear to have been derived from a lysine tRNA
1, (rabbit) gene (78% homology) and a glutamic acid tRNA (
Drosophila) gene (74% homology) (not counting the aminoacyl stem region), respectively. The homologies extended to secondary structures, homologous nucleotides being located on similar secondary structures. The idea is proposed that many, if not all, highly repetitive and transcribable (Hirt) sequences detected by total DNA transcription have their own specific tRNA genes as their progenitors.
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