Proceedings of the Japan Academy, Series B

Ion Cyclotron Waves Associated with ssc and si

Yoshio KATO, Yutaka TONEGAWA

1992 Volume 68 Issue 4 Pages 37-40
Published: 1992
Released on J-STAGE: October 13, 2006

DOIhttps://doi.org/10.2183/pjab.68.37

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It has been already reported by the present authors that ion cyclotron waves in the frequency range from 0.1Hz to 1.0Hz are generated at the time of storm sudden commencements (ssc's) and the following initial phase of the magnetic storms. In the present paper, the ion cyclotron waves are also shown to be associated with sudden impulses (si's) which are not followed by storm-type disturbances. Our conclusion is that the ion cyclotron waves are excited by gyroresonant instabilities which are caused by a compression of the magnetosphere at the times of both ssc and si.

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Induction of Conspicuously Long Extensions of Dictyostelium Cells by Protein Kinase Inhibitors K252a and Staurosporin

Mineko MAEDA

1992 Volume 68 Issue 4 Pages 41-46
Published: 1992
Released on J-STAGE: October 13, 2006

DOIhttps://doi.org/10.2183/pjab.68.41

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K252a and staurosporine, protein kinase inhibitors, induced the outgrowth of extremely long extensions by Dictyostelium cells. Histochemical observations revealed that F-actin was selectively localized in the extensions. Cytochalasin D inhibited formation of the extensions, but nocodazole did not. These findings indicate that the inhibitors altered F-actin-based architecture. During induction of the extensions, a 66-kDa protein whose amount in the Triton-insoluble fraction was increased by the inhibitors was found, though the amounts of F-actin and myosin heavy chain remained constant. Thus, this 66-kDa protein may be involved in the alteration of cytoskeletal architecture observed here. This possibility is discussed in relation to protein phosphorylation. In conclusion, the construction of cytoskeletal architectures resulting from F-actin elongation and bundling is regulated by a protein kinase(s) that is inhibited by K252a and staurosporine.

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Simultaneous Fixation of Orientation of Myoglobin and Metalloporphyrins by Synthetic Bilayer Membrane

Itaru HAMACHI, Shunsaku NODA, Hiroaki IWASAKI, Toyoki KUNITAKE

1992 Volume 68 Issue 4 Pages 47-50
Published: 1992
Released on J-STAGE: October 13, 2006

DOIhttps://doi.org/10.2183/pjab.68.47

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Simultaneous immobilization of myoglobin protein (Mb) and synthetic porphyrins was conducted by using synthetic bilayer membrane composed of a phosphat-bearing amphiphile. Electron spin resonance (ESR) spectra of the composite cast film containing Mb and copper porphyrins clearly demonstrate that molecular orientation of Mb and porphyrins against the Bilayer surface are controlled independently.

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A New Method for Isolation of Rh Polypeptide mRNAs Using a Liquid Culture System

Eiji KAJII, Shigenori IKEMOTO

1992 Volume 68 Issue 4 Pages 51-56
Published: 1992
Released on J-STAGE: October 13, 2006

DOIhttps://doi.org/10.2183/pjab.68.51

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A selective two phase liquid culture system for erythroid progenitors was developed to isolate and analyze mRNAs encoding erythrocyte-specific blood group antigens. The feasibility of the technique was verified by isolation and identification of mRNA for Rh polypeptide using polymerase chain reaction accompanied with the subcloning and sequencing methods. Two kinds of Rh polypeptide-specific cDNA sequences were demonstrated. It was revealed that each individual has one or both of them. This approach based on the culture system is widely applicable to studies to detect gene expression of any proteins of erythroid blood group antigens at the RNA level.

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