The characteristics of Ca
2+ activated K
+ channels of enzymatically dissociated single smooth muscle cells of vas deferens of the adult guinea pig were studied by the whole cell clamp technique. A stepwise depolarization to -30mV induced a fast transient current followed by the spontaneous transient outward currents (STOCs). STOCs were eliminated by application of K
+ channel blocker TEA. STOCs appeared only when Ca
2+ was present in extracellular medium, and disappeared immediately once Ca
2+ was removed from the medium. Therefore, STOCs in the present preparation can be ascribed to the activity of Ca
2+ activated K
+ channels. Furthermore, even in the absence of Ca
2+, STOCs were evoked transiently by application of caffeine, indicating the involvement of the Ca
2+ induced Ca
2+ release (CICR) mechanism. Thus, it is likely that Ca
2+ activated K
+ channels are operated via CICR mechanism and extracellular Ca
2+ is essential for keeping their constant activity.
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