Fatty acids were released from the total lipids of
Helicobacter pylori by acid methanolysis and analyzed by gas liquid chromatography and mass spectrometry. Eight peaks of the fatty acid methyl esters were detected and identified to be tetradecanoic, hexadecanoic, octadecanoic, octadecenoic, octadecadienoic, cyclo [11, 12] methylene octadecanoic, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic fatty acids. A small amount of hydroxy docosanoic acid was also detected after trimethylsilylation. Two fatty acids, 11-methoxy-heptadecanoic and 11-methoxy-nonadecanoic acids, are unique and the occurrence of these fatty acids in bacteria as well as
Helicobacter pylori is reported for the first time.
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