The effects of the polarity of various water-immiscible organic solvents on the enzymatic esterification activities of three modified
Candida cylindracea lipases, which were prepared by the chemical modification of lipase with poly[(
N-acetylimino)ethylene] (
2a), poly[(
N-propionylimino)ethylene] (
2b) and poly[(
N-isobutyrylimino)ethylene] (
2c), were examined and their enzymatic activities were tried to correlate to the logarithm of the partition coefficient of the solvent between water and 1-octanol (log P). It was found that although the activity increased with increasing the hydrophobicity of the solvent and with increasing the hydrophobicity of the
N-substituent of
2, a great rate enhancement of the enzymatic activity was achieved in benzene and toluene by the chemical modification by
2b or
2c, while unmodified lipase and
2a-modified one were almost inactive in them. Moreover, the effect of water concentration in organic solvent was investigated.
View full abstract