1) This enzyme was extremely unstable in the presence of oxygen, and this instability was completely avoided when stored in a Thunberg tube evacuated and filled with nitrogen gas.
2) This enzyme was most stable at pH 7.4 in both crude and purified state.

抄録全体を表示

The reaction, from pyridine-hemichromogen to 656mμ substance, was inhibited by the metal binding agents and SH-inhibitors.

抄録全体を表示

Inhibition experiments by sugars were carried out on hemagglutination of bacterial polysaccharide-coated rabbit red Table II. Inhibition by sugars of hemagglutination of polysaccharide-coated rabbit red cells by factor sera and precipitation of bacterial polysaccharides by factor sera cells by factor serum and on precipitation of bacterial polysaccharide by factor serum, and the following results were obtained concerning the antigenic determinants of phage-mediated antigens in Salmonella:
1. The antigenic determinant of somatic antigen 1, 7)13) which is produced in Salmonella groups A, B, and D by the phage conversion, is considered to be α-glucosyl-(1→6)-galactose-mannose-. The antigenic determinant of somatic antigen 12, 13) which is related to the infection of this phage, was assumed by Stocker, Staub, Tinelli, and Kopacka3) to be α-glucose-(1→4)-galactose-mannose-rhamnose. It is consequently presumed that the difference between somatic antigens 1 and 12 would be only the difference in the form of combination between glucose and galactose.
2. In Salmonella group E, somatic antigen 3, 10 is converted into 3, 15 by means of phage ε 15.8) Both the phage-mediated antigen 15 and the original antigen 10 are considered to have β-galactosyl-(1→4 or 1→6)-glucose-mannose- as their determinants, and it is difficult to distinguish them from each other. Furthermore, in Salmonella group E, antigenic conversion takes place from 3, 15 into (3), (15), 34 by means of phage ε 34.10) The antigenic determinant of the phage-mediated antigen 34 is considered to be α-glucosyl-(1→4)-[galactose or glucose]-. In this way, there is the difference between the somatic antigen 15 and the somatic antigen 34 in the end determinant.
3. In Salmonella group C, somatic antigen 20 is added to 6, 8 by means of phage.9) And this phage-mediated antigen 20 is considered to have N-acetylglucosaminoyl-(1→4 or 1→6)-glucose as its determinant.

抄録全体を表示

In Shigella flexneri, antigenic conversion takes place Table II. Inhibition by sugars of hemagglutination of bacterial polysaccharide-coated rabbit red cells by factor sera through the mediation of bacteriophage from type antigen I, II, and III into IV₁, and from II, IV₁, X, and Y into I. These type antigens were examined for their antigenic determinants by means of absorption of factor serum by sugars. According to the results, it was difficult to find the difference between antigenic determinants of type antigens I, II, and IV₁, all appearing to be α-glucosyl-(1→4)-[galactose or glucose]-. and that of type antigen III would probably be β-glucosyl-(1→6)-[galactose or glucose]-.

抄録全体を表示