The experimental salmonellosis in the relation between mouse and
S. enteritidis was studied and the following results were obtained;
1)Mouse super-immunized with live vaccine acquired high resistance to the infection with the virulent strain, and the mouse survived intravenous infection with 1, 000 MLD of a virulent strain 116-54 without loss of body weight. But the mouse immunized with dead vaccine did not. 2) In vitro infection with S. enteritidis of mononuclear phagocytes derived from the abdominal cavity of the mouse immunized with dead vaccine showed that the intracellular multiplication of a virulent strain 116-54, and the phagocytes were destroyed after 3 days' incubation. 3) The serum obtained from the mouse immunized with dead or live vaccine has no inhibitory effects on the intracellular growth of a virulent strain 116-54 in the normal mouse phagocytes. 4) The mononuclear phagocytes derived from the mouse super-immunized with live vaccine inhibited the intracellular growth of a virulent strain 116-54 regardless of the presence of antibody in the tissue culture medium.
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