1. By zymograms of the polyacrilamide-gel electrophoresis, the new two zones, Af and Cs-zones were recognized in addition to the same three zones as A, B and C-zones which were reported by Rendel et al. 2. The frequencies of appearance of alkaline phosphatase Af, A, B, C and Cs were as follows: (1)Af-zone was recognized in adult sheep only. The frequencies of appearance of Af-zone in a few populations were 0-40%, and a slight difference due to breeds was observed. (2) A and C-zones were observed in all kinds of sheep. (3) The frequencies of appearance of B-zone were about 80%. (4) Cs-zone was rarely observed in adult sheep only. 3. The intensities of A and B-zones in lamb were much stronger than those in adult sheep. Also, that of C-zone in lamb was much weaker than that in adult sheep. 4. B+ type is dominant to B- type. 5. The symbols of the dominant and the recessive genes of phosphatase were named APase B and APase b, respectively.
Existence of two blood types in red cells of the goats were clarified by extract from leaves of Vicia unijuga. It was apparent that the frequencies for positive type in male and female were 63.4% and 68.7% respectively.
Patients' sera with different history of immunization were tested for leukocyte antibody titer by the latex agglutination. Most of the normal persons were negative except for some cases giving weak agglutination up to titer 8. Patients who received blood transfusions showed higher agglutinating activities; the bone marrow transplanted group gave the highest anti-leukocyte titers.