Human diploid cell cultures from adult male liver were treated with thioacetamide. The control cultures died at around 23 subculture passages. The treated series grew more than double the length of the normal lifespan with noticeably different growth patterns and cells changed into a tetraploid range. The treated cells showed cell aggregates in a rotation culture. No tumorigenicity was found.
A C-banding technique applied to the second meiotic spermatocyte metaphases affords an estimate of the frequency of adjacent-2 and 3: 1 disjunctions at the first meiotic division in male T (1;13) 70H heterozygotes. The frequency of adjacent-2 disjunction is about 30% and that of 3:1 disjunction 4%.