Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
Volume 26, Issue 2
Displaying 1-13 of 13 articles from this issue
Contents
  • KATSUHIKO SUZUKI
    1980 Volume 26 Issue 2 Pages 158-169
    Published: June 10, 1980
    Released on J-STAGE: November 21, 2014
    JOURNAL FREE ACCESS
    Actin was obtained in high purity and with a high yield from chicken gizzard muscle. 1) The amino acid composition of gizzard actin was almost identical with skeletal actin. Gizzard actin contained approximately 6 cysteinyl residues per mol of actin, although skeletal actin had 5 cysteinyl residues per mol. 2) Two forms of polymerized actin, F-type and f-type, were obtained depending on whether or not calcium ions were present in the medium used for extraction and purification. F-type actin was almost identical with skeletal muscle F-actin in sedimentation coefficient, viscosity, and activation of the Mg2+ ATPase of myosin. f-type actin showed charactristics similar to those of plasmodium Mg-polymer; low viscosity, steady ATP-splitting and electron micrographic appearances. 3) When dialyzed aginst a buffer without added calcium, gizzard G-actin which was otherwise capable of polymerizing to F-type, was transformed to f-type-forming G-actin, with concomitant loss of a considerable amount of bound calcium. 4) Skeletal and gizzard actins were cleaved by 2-nitro-5-thiocyanobenzoic acid at cysteine residues, and then analyzed by SDS-polyacrylamide gel eleotrophoresis. At least two fragments of cleaved gizzard actin showed similar but nonidentical electrophretic mobility as compared with the corresponding fragments of skeletal actin.
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  • JIRO KOBAYASHI
    1980 Volume 26 Issue 2 Pages 170-182
    Published: June 10, 1980
    Released on J-STAGE: November 21, 2014
    JOURNAL FREE ACCESS
    The measurement of anti-native DNA (n-DNA) and denatured DNA (d-DNA) antibodies in sera from patients with SLE and other rheumatic diseases ussng millipore filter redioimmunoassay was studied methodologically and clinically. The 125I-n-DNA and d-DNA produced by the in vitro labelling of calf thymus n-DNA and heat denatured DNA with 125I. Radioactive DNA was separated from other reactants by passage throgh a Sephadex G-50 column. The specific activities of 125I-n-DNA and d-DNA were 0.3μCi/mg and 0.94μCi/mg, respectively. Test sera were heated at 56°C for 30 minutes. Fifty microliters of heated serum were added to labelled DNA in 200μ liter of 0.15 M Tris buffer, PH7.5, and 150μ liter of distilled water.The assay mixture was incubated at 37°C for 15 minutes afeer which the reaction was stopped by the addition of 4ml of SSC solution (mixture of 0.15M Sodium Chloride and 0.015M Solium Citrate). The diluted assay mixture was passed through a prewetted millipore filter (type-HAWP) under gentle suction. The radioactivity of the filter membrane was measured in a scintillation counter, Sera were obtained from 32 healthy controls, 22 patients with rheumatic diseases other than SLE and 33 patients with SLE. As the results, the millipore filter assay using preheated sera was capable of detecting specific heat stable DNA binding activity in SLE sera. Complex formation between radioactive DNA and anti-DNA antibodies in SLE serum was biphasic, with a rapid initial phase complete in 10 minutes. Optimum. density of salts in Tris buffer was determined to be 0.15M. When unlabelled n-DNA and d-DNA were added to 50μl of serum from the SLE patients with high titer of anti-n-DNA and-d-DNA antibodies, unlabelled n-DNA inhibited the binding of radioactive n-DNA, and unlabelled d-DNA inhibited both the binding of radioactive n-DNA and d-DNA. The high levels of anti-n-DNA antibodies were found in sera from patients with SLE and reflected the degree of disease activity. On the other hand, anti-d-DNA antibodies appeared in sera from not only SLE but other rheumatic diseases. This simply and rapidly perfomed assay is easily quantitied and can be useful in the diagnosis and management of patients wit SLE.
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  • TAKEO ARAI
    1980 Volume 26 Issue 2 Pages 183-197
    Published: June 10, 1980
    Released on J-STAGE: November 21, 2014
    JOURNAL FREE ACCESS
    Fourty cases of autogenital bilary atresia were examined histopathologically, concering the index of hepatomegaly and splenomegaly, histopatholgical findings of the liver and the spleen, grading of the hepatic fibrosis and the sinus hyperplasia of the spleen. Histometrical study on the splenic sinus by Suwa's methood (application of Weibel's method) were also done. Additional splees (7 cases of so-called Banti's sydrome of the second decade, 16 cases of so-called Banti's syndrome of the adult as control, and 20 infants, 7 teen-aged and 23 adult cases as normal control) were investigated histometrically to compare with CBA. Results : 1) Severe biliary fibrosis with marked obliteration of the intrahepatic portal veins were in all cases (mean obliteration rate 45.4%) 2) Progressing splenomegaly were seen (mean splenomegaly index 3.52, maximum 10.27) 3) Histopathological findings of the spleen in CBA were very similar to that of so-called Banti's syndrome (Fibrous thickening of splenic capsule, peritrabecular congestion and hemorrhage, deposition of the iron around the trabeculae, parifollicular congestion and hemorrhages, follicular and perifollicalr fibosis, marked periarterial fibrosis, proliferation of the argentaffin fibers with widening of the splenic cord and varoius stage of sinus hyperplasia) 4) Splenic sinus in CBA become finer and longer than that of normal infant control according to the progress of sinus hyperplasia caused by increased portal pressure. 5) 24 cases (60%) of esophgeal varicose vein, 4 cases (10%) of its rupture and 23 cases (57.5%) of localized intimal thickening of the splenic veins were seen. 6) Histometrical study : Alanough the splenic sinus in the teen-anged of normal control was the longest and most spacuous in normal control, the splenic sinus in portal hypertedsion become finer finer and longer according to their aging. It is concluded that the splenic changes in congenital biliary atresia was similar to that of so-called Banti's syndrome quantatively, after due consideration of growing infant spleen.
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