Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 27, Issue 3
Displaying 1-12 of 12 articles from this issue
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Contents
  • Morphological Studies on Cultured Cardiac Cells of New-born Rats.
    MASAHIRO OGAWA
    1981 Volume 27 Issue 3 Pages 290-304
    Published: September 10, 1981
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.27.290
    JOURNAL FREE ACCESS
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    The hearts of new-born rats (2-day-old, Wistar strain) were cultured using by Harary and Farley's method. The re-organization of cultured cardiac cell was studied by phase contrast microscopy and by scanning and transmission electron microscopy. Cultured cardiac cells were stained with the PAS-reaction and stained for acid phosphatase activity. Differenciation of the general working cardiac cell and the conduction system cell was histochemically examined. The results are as follows : 1) The round or polygonal cells with many microvilli on their surface were cardiac cells. The cells were connected with adjacent ones by long cytoplasmic extensions. On the third day of culture, cell clusters organized with single cells were formed and developed into network structure on the eighth day of culture. 2) In view of their reaction to saliva, PAS-positive granules were considered to be glycogen ones in cultured cardiac cells. PAS-positive cells were of two kinds; strongly or slightly positive one. Strongly PAS-positive cells were large and polygonal and had high acid phosphatase activity. This kind of cells were mainly cultured from the free wall of the left ventricle of new-born rats and were morphologically identified as Purkinje cells. The triangular cells with clear zones, stained positively with the PAS reagent, had also high acid phosphatase activity. This kinds of cells was obtained from the atrioventricular node. On the other hand, the round PAS-positive cells with low acid phosphatase activity were considered to be general working cells. The spindle-shaped cells with few PAS-positive granules and few or no glycogen granules were endotherial cells.3) Histochemically, the cells with high acid phosphatase activity were well-stained with the PAS reagent. Therefore, between these cultured cardiac cells the conduction system cells are presumed to have higher anaerobic glycolytic activity than the general working cells.
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  • Clinical and Histo-Pathological Studies on Congenital Biliary Atresia
    Part 1. Liver cell change and prognosis
    YASUNORI KOJIMA
    1981 Volume 27 Issue 3 Pages 305-315
    Published: September 10, 1981
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.27.305
    JOURNAL FREE ACCESS
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    The histo-pathological studies on 84 resected livers taken at the radical operations of congenital biliary atresia were concluded that conditions of liver cell were devided into 3 groups (Cn-classification) according to nuclei number of liver cells in (130μ) 2; i.e. C1 : _??_37 (40 cases), C2 : 38_??__??_48 (37 cases), C3 : 49_??_ (7 cases). Nine autopsy infantile cases and 5 cases of resected livers were studied as the control groups. Most cases of the C1 and C2 groups showed slight (F1) to moderate (F2) liver fibrosis. Most cases of C1 and C2 groups showed more than 100μ inside diameter of hepatic bile duct at porta-hepatis. Most cases of C1 or C3 group showed younger or older operative age, respectively. Clinicall, 22 out of 25 cases (88%) of C1 and C2a (the subtype in C2 group) groups showed good prognosis. Most cases (85.7%) of C3 group showed worse prognosis.
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  • Histopathological Studies on Gastric Adenosquamouscarcinoma
    SHIGEHIRO KITAMURA
    1981 Volume 27 Issue 3 Pages 316-329
    Published: September 10, 1981
    Released on J-STAGE: November 21, 2014
    DOIhttps://doi.org/10.14789/pjmj.27.316
    JOURNAL FREE ACCESS
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    Adenosquamous carcinoma or squamous cell carcinoma arising primarily in the stomach is a rare tumor (an incidence is 0.1-0.4% of whole gastric carcinoma), and in Japan 109 cases were described in the literature. There were three theories of the histogenesis on adenosquamous carcinoma. 1) Malignant change of squamous epithelium in the stomach (squamous metaplasia of gastric mucosa or ectopic squamous epithelium). 2) Squamous metaplasia of a preexisting adenocarcinoma. 3) A totipotential cell (undifferentiated carcinoma) capable of differentiating to any cell type. The author classified eleven cases (five autopsy cases) of gastric adenosquamous carcinoma to two types of coexistent adenocarcinoma, well differentiated type and undifferentiated type, and discussed to the histogenesis of adenosquamous carcinoma of the stomach. In five cases, coexistent with well differentiated adenocarcinoma, squamous cell carcinoma was seen in the deep layer of cancer cell nest and not seen in mucosa. Histologically, squamous cell carcinoma was found in the basal side of carcinomatous gland, and poorly differentiated cancer cells were also observed. In six cases, coexistent with undifferentiated adenocarcinoma, squamous cell carcinoma was seen in the mucosa and also seen in any place. Histologically, squamous cell carcinoma was found in the undifferentiated adenocarcinoma and was forming cells islet. Undifferentiated squamous cell carcinoma cells were connecting with the undifferentiated adenocarcinoma and were transformed each others. It was considered that squamous elements arise from preexisting well differentiated adenocarcinoma in the former type, squamous element arise from one of multipotency of undifferentiated carcinoma in the latter type.
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