Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
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Volume 42, Issue 2
Displaying 1-20 of 20 articles from this issue
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Contents
  • Voltage-gated K+ and Ca2+- activated K+ currents developmentally expressed in the H9c2 clonal cell line from rat heart
    WANG WEI
    1996 Volume 42 Issue 2 Pages 191-199
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.191
    JOURNAL FREE ACCESS
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    The properties of the outward K+ currents of H9c2, a clonal cell line derived from rat heart, at both the undifferentiated myoblast and differentiated myotube stages were characterized using the patch clamp technique under whole-cell clamp configuration. Subjecting myoblasts to depolarization steps activated a voltage-gated outward K+ current (IKV), which showed a rapid rise, a slow decay and steady-state inactivation by depolarization of the holding potential. The myoblastic IKV was suppressed by exogenous tetraethylammonium with a 50% inhibition concentration (IC50) of 1.9mM. Subjecting myotubes to depolarization steps generated other K+ currents with much slower activation rates followed by slow tail currents on repolarization. These slow currents were completely soppressed by superfusion with Ca2+-free Tyrode's solution and by intracellular application of 10mM EGTA, indicating that these were Ca2+-activated K+ currents (IKCa). High concentration of charybdotoxin (CTX, 100nM) and apamin (100nM) depressed the total IKCa by 57.0 and 43.0%, respectively, white simultaneous application of both toxins suppressed it completely. The expression of IKV and two distinct IKCa in the myotubes indicated that the differentiated H9c2 cell line exhibited outward-going K+ channel properties that were more similarto skeletal than cardiac muscle, suggesting that the gene expression of IKCa channels in these cells is regulated by a differentiation program.
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  • Ultrastructu al study of the glomerular filtration barrier in postnatal and adult rats
    HARUKO WATANABE
    1996 Volume 42 Issue 2 Pages 200-206
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.200
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    The ultrastructure of the glomerular filtration barrier was observed after cold dehydration in the adult and developing rat. In the adult, thin cytoplasm of the endothelial cells possessed fenestrations without a diaphragm. The glomerular basement membrane was composed of a single lamina densa with lamina rara externa and interna on both sides. The foot processes were connected with each other by filtration slits, and with the lamina densa by connecting fibers. The cell membrane of foot processes at the filtration slits and connecting fibers was counected with electron-dense materials on the cytoplasmic side. On day 5 after birth, the structure of the filtration barrier was immature, consisting of unfenestrated endothelium, 2 layers of laminae densae in the basement membrane, and undifferentiated podocytes without foot processes. By day 15, the filtration barrier had matured to the adult type. The maturing process began at the basement membrane. Outpockets of the glomerular basement membrane, which represented expanding sites of the basement membrane, were numerous on days 10, 15 and 20, then decreased in number on days 40 and 60. Of the two types of outpockets, the loop type was more numerous than the columnar type.
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  • Studies on cisplatin plus 5 -fluorouracil chemotherapy for the head and neck squamous cell carcinoma using multicellular tumor spheroids
    MASAE KUSUNOKI, EIKO NAKAZAWA, NAOYUKI KOHNO
    1996 Volume 42 Issue 2 Pages 207-216
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.207
    JOURNAL FREE ACCESS
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    Cisplatin (CDDP) plus 5-fluorouracil (5-FU) is an effective regimen for the treatment of patients with squamous cell carcinoma of the head and neck. We studied the efficacy of CDDP plus 5-FU combination against HEp-2 head and neck carcinoma cells in monolayer and multicellular tumor spheroid (MTS) systems. MTS measuring approximately 500μmin diameter were prepared by the liquid overlay culture technique from HEp-2 laryngeal squamous carcinoma cell lines. The cells in monolayer and MTS were exposed to the agent in graded concentrations for 1, 24 and 72 hours for CDDP and 6, 24 and 72 hours for 5-FU respectively. Then monolayer cells and MTS were trypsinized to a single cell suspension and subjected to clonogenic assay. CDDP requires at least 24 hours exposure. The cytotoxic effect of 5-FU was time-dependent. Increasing exposure time from 24 to 72 hours resulted in approximately a ten fold in crease in cell kill effects for concentrations of 5-FU 0.01μg/ml and 0.1μg/ml in both monolayer and MTS cells. Synergistic interaction was observed drug co-administration of CDDP and 5-FU, when the concentrations of CDDP and 5-FU exceeded 0.1μg/ml and 1μg/ml, respectively, MTS has certain characteristics similar to de novo solid tumors. Containing extracellular matrix such as fibronectin, laminin and collagen. MTS also are composed of a chronically hypoxic cell population at the core and show heterogeneous cell cycle times. For these reasons, we used MTS as an in vitro model to study the effects of chemotherapeutic agents.
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  • Inpatient consultation-liaison psychiatric activities at Juntendo University Hospital (Second Report)
    HIROSUKE TAKAHAMA, DAISUKE MORI, TOSHIAKI MASUMURA, TETSU KAWAMURA, NO ...
    1996 Volume 42 Issue 2 Pages 217-224
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.217
    JOURNAL FREE ACCESS
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    Inpatient consultation-liaison activities at Juntendo University Hospital were investigated retrospectively. During the last 6 years, from April 1989 to March 1995,328 inpatients (135 men and 193 women, mean age : 51.5 years old) received our psychiatric treatment. Compared with our previous study period, from April 1986 to March 1989, both the number of the consulted patients and their mean age increased. Most of those patients were referred from the internal medicine division. Psychomotor-excitement was the most frequent reason for their consultations. The most common psychiatric diagnosis was delirium. The number of the cases with depression increased. The frequency of consultation due to previous psychiatric history was decreased. With respect to the management of those patients, in addition to delirious patients, the cases with terminal stage of cancer, chronic pain or suicidal attempt needed intensive psychiatric treatment. These findings suggested the importance of the inpatient consultation-liaison activities services. Furthermore, we should work more closedy with the consultation-liaison on activities at Juntendo University Hospital.
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  • Abnormalities in the absorption and the metabolism of levodopa in Parkinson's disease
    KOZO HATORI, TOMOYOSHI KONDO
    1996 Volume 42 Issue 2 Pages 225-232
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.225
    JOURNAL FREE ACCESS
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    We report the levodopa absorption profile and its metabolism in 8 patients with early stage Parkinson's disease (PD) in comparison with 7 age matched normal control subjects (NC). Each subject took 500mg of levodopa, 10mg of domperidone to minimize nausea, and 500mg of acetaminophen to measure gastric emptying time orally in the morning, and plasma levoddopa, 3-O-methyldopa, dihydroxyphenylacetic acid (DOPAC), 3-methoxytyramine (3-MT), and homovanillic acid (HVA) were assayed by liquid chromatography. The mean initial peak concentration of plasma levodopa and the area under the curve for 8 hours (AUCo→8h) of levodopa were significantly higher in the PD patients than in the NC subjects. The mean initial peak concentration of plasma 3-O-methyldopa and the area under the curve for 8 hours of 3-O-methyldopa were also significantly higher in the PD patients than in the NC subjects. On the other hand, no significant difference was noted in the other metabolites of levodopa. No significant difference was noted in the gastric emptying time between the PD patients and the NC subjects. These findings indicate that the absorption of levodopa is increased in early non-treated PD patients, and that metabolism of levodopa to 3-O-methyldopa by catechol-O-methyltransferase is also increased. These metabolic characteristics of levodopa may be used as an early marker for the diagnosis of Parkinson's disease.
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  • Bone leisions after gastrectomy in the elderly
    KOH MORICHIKA, MASAO SHINOHARA, TAKAO SUZUKI
    1996 Volume 42 Issue 2 Pages 233-239
    Published: July 30, 1996
    Released on J-STAGE: November 18, 2014
    DOIhttps://doi.org/10.14789/pjmj.42.233
    JOURNAL FREE ACCESS
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    Although the aged population is increaing, there have been few studies of osteroporosis after gastrectomy in the elderly. This study evaluated bone demineralization after gastrectomy in patients over 65 years compared to age 1 -matched and controls by age grade within 5 years (I), 5-10 years (II), beyond 10 years (III). In addition, we idenfified further follow-up: -lumbago; muscular pain, stiffness and numbness of the extremities. Bone mineral density was measures by DIP (digital image processing). Forty-five patients (13 females mean 71 years, 32 males mean 72.4 years) were included. The incidence of bone demineralization was 69.2% in females (F), and 62.5% in males (M), by agegrups, the incidence was 60% (I) ·100% (II), 60% (III) in females, and 52.5% (I) ·50% (II) ·76.5% (III) in males. Symptoms in patients showing bone demineralization included lumbago in 71.4% (F.M), muscular pain in the extremities in 72.7% (F): 71.4% (M), stiffness in the extremities in 85.7% (F): 75% (M), and numbness in the extremities in 83.3% (F): 50% (M). Bone demineralizaion and related symptoms were noted in patients with abvancing age. After gastrectomy, elder lypatients be monitored for loss of bone mineral density and ed as necessary on a case by case basis.
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