Juntendo Medical Journal
Online ISSN : 2188-2134
Print ISSN : 0022-6769
ISSN-L : 0022-6769
Search
Advanced search
OR
Browse
Search
Volume 7, Issue 1
Displaying 1-16 of 16 articles from this issue
Show all abstracts
    • |<
    • <
    • 1
    • >
    • >|
  • Studies on the Clinical Examination of Tubercle Bacilli in Sputa, Especially a Statistical Observation on the Cultured Results of Negative Samples from Smear Method
    Hideo Ohta
    1961 Volume 7 Issue 1 Pages 1-20
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.1
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The laboratory records of findings in tubercle bacilli detection from the patients' sputa in a tuberculosis hospital in the suburbs of Tokyo, were analysed from the statistical point of view. Period studied was about six years from August, 1954 to April, 1960. Modified Ziehl-Gabbet's staining method and fluoro-microscopic method were used for smear examination, and for cultivation, preoperated by 8% NaOH, samples were incubated in 3% KH2PO4 Ogawa's media. Main findings were as follows : (1) Culture method was superior to smear method for the detection of tubercle bacilli, especially in the case of negative samples from smear method. (2) Number of Gaffky's scale, duration until the colony of bacillus was visible, number of colonies at each time of observation and type of colonies were related each other in directly or reversely. (3) Cultivation of negative samples from smear method showed some interesting results. Distribution of number of colonies at 13 weeks after incubation was almost logarithmic normal. Deviation was quite large compared with that of normal distribution but the number of bacillus that was able to form colonies was estimated as extremely small. (4) Statistical analysis of the growth curves of colonies of the above samples, indicated that there was no relation between the final number of colonies and the growth curve, and that the rate of growth was highest at the initial stage after incubation, followed by the rapid decrease by week in geometric progression. (5) Some findings were mentioned on the resistance to one. two or three drugs at several concentraion rates.
    View full abstract
    Download PDF (2712K)
  • Studies on a New Method of Dye-Test in the Classification of the Species of Brucella
    Syunnosuke ATSUTA
    1961 Volume 7 Issue 1 Pages 21-28
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.21
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Many methods have been used to classify the three species of Brucella. For instances, to measure the metabolism in nitrogen and dextrose, to recognize the reduction of nitrate and nitrit, to observe the production of H2S, or to test the resistance of the organisms against several dyes, these methods were most commonly practised in many laboratories. Among the several methods, the dye-test which reported by Huddleson was one of the most useful method in classifying Brucella, and the author eliminated some inconveniences in this method by the application of the modified Szybalski's agar-gradient plate. In the author's method, it was able to observe the resistance of the organisms at the more dilluted point of concentration, consuming fewer culture media.
    View full abstract
    Download PDF (1065K)
  • Analysis of sportsman's physique
    Shigeo Kasai
    1961 Volume 7 Issue 1 Pages 29-44
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.29
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    This study maked research in sportsman's somatotype in college of Japan, and this research method was depend on the Analysis of Sheldon's somatotype.
    View full abstract
    Download PDF (1992K)
  • Physiological Research of Interval Training
    Masaichi ASAKAWA
    1961 Volume 7 Issue 1 Pages 45-54
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.45
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Concerning to physical effect of interval training, 1) This training braing to develope respiration and bloodcirculation systems. 2) Oxygen debt is require more in first recovery term than in training term. 3) Pulse decreased 30 seconds to 1 minute in training over. It is most important things to determine the interval training rest. 4) Pace endurance work and Speed endurance work required O2 Uptake abilities and Long interval training required O2 debt abillities.
    View full abstract
    Download PDF (1374K)
  • Investigation of Foreigen Bodies in Living Animals by the Window Method (II)
    Jiro MINAGAWA
    1961 Volume 7 Issue 1 Pages 55-63
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.55
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The smadl window method is a usefull method, for the comparison of the inifial reaktions caused by various types of foreigen bodies placed into the sudcufaneous fissue of rabbits. By this method, the comparison was made between the reaktions caused by some new plastics ('ERICON' and Ventriculo-Anial Values) Placed into the subcufaneous fissue, and they were found to be less lrritable. It was clear by this method, that the Exsudat in inflammetion caused by borign body was deeredsed by local injection of Hydrocorfitione or Predonicolon.
    View full abstract
    Download PDF (3546K)
  • Histrical Studies of Paraffinoms
    Jiro MINAGAWA
    1961 Volume 7 Issue 1 Pages 64-68
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.64
    JOURNAL FREE ACCESS
    Download PDF (1759K)
  • X ray Studies in Tibia Curvature of Normal Infants in Japan
    Ikutaro KAMEDA
    1961 Volume 7 Issue 1 Pages 69-84
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.69
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Diagnosis of pathological genu varum in infants is difficult due to the fact that the degree of physiological bending of the tibia on X-ray film has not been investigated statistically. X-ray examinations have been carried out on 328 healthy infants to analyze the development and physiolgical bending of the tibia and their changes with age (month), and the following conclusions were reached. 1. Because of the remarkable and rapid changes in the development of infant tibia, monthly examination is required to know the physiological conditions. 2. The development of the tibia is more rapid in younger infants, and is most marked in newborns under 3 month of age. 3. In order to know the degree of bending of tibia, roentgenography on the lower extremities in the standard position (stretching the bilateral lower extremities together in parallel with the patellae facing straight forward) is necessary. The bending appears to be lessened when the tibia is more inwardly rotated, while it is exaggerated and appears similar to genu varum when the tibia is more outmardly rotated. 4. The site of bending is usually located nearer to the upper end of the bone than at the metaphysis. Upper third of the shaft of the bone is comparatively straight and increases in its length gradually with age. 5. The lines connecting A and B at the proximal end of the tibia and C and D at the distal end form an angle (∠α) at the median side of the bone. Since the angle (∠α) is correlated with the indices (PQ/MN×100) and (PQ/AC×100=bending index), the dgeree of bending may be exbressed quantitatively by ∠α. 6. The values of ∠α obtained from the study indicate that physiological inward bending is present in newborn babies which derreases in this severity rapidly within one month after birth and thereafter, gradually with age. A mild increase in bending is again observed in 16-18 months of age which is considered to be due to the load of body weight on the lower extremities at the onset of standing and walking.
    View full abstract
    Download PDF (3641K)
  • One Case of Interesting Pubic Fracture
    Toyozi OKAMURA, Ikutaro KAMEDA, Ko OHAYASHI, Takeshi WATANABE
    1961 Volume 7 Issue 1 Pages 85-86
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.85
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Three years old female case of the pubic fracture was reported. By the clinical and roentogenological findings, this case was recognized as a Juxta epiphysal fracture.
    View full abstract
    Download PDF (602K)
  • Effects of Several Drugs on the Blood-gases of Rabbits Exposed to High Environmental Temperature
    Fujio UCHIDA
    1961 Volume 7 Issue 1 Pages 87-97
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.87
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Rabbits were exposed to a hot environment, 42 to 45°C. for one hour, and the arterial blood was taken before and after the exposure. O2-and CO2-content, alkali reserve, hematocrit, hemoglobin concentration and pH were examined. The following results were obtained: 1. In rabbits exposed to hot environmemt, rectal temperature were elevated, O2-content decreased, CO2-content decreased, Sa-O2 slightly increased, alkali-reserve markedly decreased, Hb-content and Ht increased, and pH of whole blood decreased. 2. Parenteral administration of V.B1, V.C, Cortisone, and sodium citrate were all effective in correcting the adove mentioned changes of bood-gases of rabbits exposed to hot environment. 3. V.B1 and sodium citrate were more effective in correcting the changes of blood gases. 4. Beneficial effect on Hb-content and Ht were most manrked in cases of cortisone, and less in cases of V.B1 and V.C. and much less in cases of sodium citrate.
    View full abstract
    Download PDF (1520K)
  • Relationship between the Toxic Effect of Nitromin (Nitrogen Mustard N-oxede) and Development of Resistance to it
    Isao MORITA
    1961 Volume 7 Issue 1 Pages 98-111
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.98
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    I have studied the acute, subacute and the chronic toxic effects of NMO (Nitromin: Methyl-bis (β-chloroethylamine) N-oxide hydrochloride), especially in the view point of haematology and patho-ana-tohistology, with healthy rabbits, rats and mice. Then, experimental studies have been undertaken in the hope of establishing the develop of a resistant subline of Yoshida-sarcoma by connective passage of sarcoma in Oosawa strain rats receiving continous injecteon of NMO.: following result were performed. 1) During the succesive injection of NMO, leucopenia and following anemia were noticed, at the same time anatomical findings also showed severe panmyelophthisis, desolution of spermatogenesis, atrophy of spleen with degeneration, lymphrtic gland and thymus and ulcer with edema in the large intestine. But some of them showed remarkable recovery in blood picture, and their bone marrow, spleen and lymphatic gland were rather more hyplastic and leucemoid. 2) According to marked shortning of the survival time of rats inoculation with sarcoma inspite of the injection of NMO, it has been demonstrated that a resistant subline of sarcoma to NMO has been developed relativ easely, and that the subline has showed a tendency towards more rapid growth receiving injection of NMO. 3) Some relationship were recognized between the development of resistance to NMO in bone mallow, testis and intestinalepithelium of healthy animals, and the develpment of resistant variant of Yoshida-sarcoma. These resistance were thought as a variation of reaction-mood of the cells to the NMO during the continous injection of the agent. These findings favor the assumption that inhibitiv effect in cancerous growth can be changed to its growth promoting effect in NMO-treatment.
    View full abstract
    Download PDF (5390K)
  • Studies on the Cell Nucleus of Yeasts.
    (I) Stainability of the Intracellular Structures.
    Heiji MARUYAMA
    1961 Volume 7 Issue 1 Pages 112-119
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.112
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Intracellular structures of Saccharomyces cerevisiae (diploid and haploid strains) and Candida robusta (proved to be triploid strain of Saccharomyces by Takahashi, 1959) were investigated with several nuclear and mitochondrial staining dye solutions. Staining procedure used were, HCl-fuchsin, HCl-thionin, HCl-toluidine blue, ribonuclease digestion method, supravital staining with methyl green, neutral red, janus green and toluidne blue, and many combinations with these procedures. Results obtained were summarized as follows : 1) So called cell nucleus of the yeasts were obviuosly different from mitochondria. 2) Number of the structure stained by nuclear staining dyes did not increase parallely to the ploidy. 3) Nuclear substance exist in the area surroundig the vacuole, or diffusely dispersed nuclear substance accumulate in the perivacuolar area in the process of the staining procedure. And consequently shrinkage of the vacuole occured resusting in formation of chromosome-like nuclear configurations.
    View full abstract
    Download PDF (2717K)
  • Studies on the Cell Nucleus of Yeasts. (II)
    Relationship between the Chromosomal Situation and the Variation rate of Biochmical Characteristics.
    Heiji MARUYAMA
    1961 Volume 7 Issue 1 Pages 120-128
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.120
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Biological characterisitcs of Saccharomyces cerevisiae and Candida robusta, including haploid and diploid strains, were investigated by the colonial dissociation method aand variation rate in sugar fermentation and sugar assimilation was surveyed. Results were as follows : 1) By adding yeast extract to the basal medium for the sugar assimilation test, the inoculum could be reduced as much as 1/2 to 1/4 of the usual method. 2) Using the improved half slant medium, accuracy of the sugar fermentation test was increased, Moreover, by this method, many of the non fermentor were proved to be formentable of the used sugar. i.e. in the non fermenter, many latent fermentters were recognized. 3) Variation rate of both fermentation and assimilation reaction were so high that these charateristics did not seem to be controled genetically by so called mitosis of the chromosomes. 4) Referring to the results of the previous report, it comcluded that the nuclear substance of the yeasts did not exist as chromosomes.
    View full abstract
    Download PDF (1257K)
  • The Effect of Sucrose-Phosphate-Glutamate (SPG) Solution on the Experimental Infection with Rickettsia orientalis
    Takahiro SUGAWARA
    1961 Volume 7 Issue 1 Pages 129-136
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.129
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    The effects of saline and sucrose-phosphate-glutamate (SPG) solution on the experimental infection with Ozeki strain of Rickettsia orientalis were examined. Two sorts of emulsion were made from pooled spleens of infected mice, the one using saline as diluent and the other using SPG-solution. Their infectivity for mice was tested by intraperitoneal injection. 1. SPG-emulsion seemed more favorable in preservation of viability of Rikettsiae in vitro at room temperature than saline saline-emulsion. 2. The log LD50 of SPG-emulsion was greater than that of saline-emulsion. 3. The dosage of Rikettsiae which is sufficient to cause the infection in mice when SPG was used as diluent did not cause the infection when saline was used as diluent. 4. The absorption of Rickettsiae in SPG-emulsion from abdominal cavity was faster than in saline-emulsion. 5. It appeared that, when mice was injected with same dosage of Rickettsiae as SPG-emulsion and saline-emulsion respectively, the survival time of mice was shorter in the latter ; and this tendency seemed more distinct in cold enviroment.
    View full abstract
    Download PDF (1524K)
  • Effect of Environmental Thermal Factors on the Experimental Infection with Rickettsia
    Shigeru ISHIHARA
    1961 Volume 7 Issue 1 Pages 137-145
    Published: February 10, 1961
    Released on J-STAGE: November 22, 2014
    DOIhttps://doi.org/10.14789/pjmj.7.137
    JOURNAL FREE ACCESS
    Show abstractHide abstract
    Effects of environmental thermal factors on experimental infection in mice with Ozeki strain of Rickettsia orientalis were examined. The infectivity of the rickettsiae whice passaged through cold acclimatized mice and moderate temperature acclimatiged mice were assayed for their LD50 value and survival time for mice by intraperitoneal injection ; the rickettsiae passaged through cold acclimatized mice showed greater LD50 value and shorter survival time. After intraperitoneal injection with same same dosage of rickettsiae three groups of mice were exposed to cold, moderate and hot environment and the difference in susceptibility between them was studied ; cold-exposed group showed greater LD50 value and shorter survival time than moderate-temperture-exposed group, and hot-exposed group showed smaller LD50 value and shorter survival time than moderate-temperature-exposed group. The susceptibility for rickettsiae infection of mice acclimatized to the thermal environment, to which mice were exposed after injection, was observed and compared with that of non-acclimatized mice. While in non-acclimatized mice the group exposed to cold environment showed greater LD50 value and shorter survival time than the group exposed to moderate temparature, in acclimatized mice the cold-exposed group showed slightly greater LD50 value than moderate-temperature-exposed group, but survival time of the former group was not always shorter than the latter This results suggest the effect of acclimatization on the susceptibility of mice.
    View full abstract
    Download PDF (1512K)
    • |<
    • <
    • 1
    • >
    • >|
feedback
 Top