The genes encoding chalcone synthase (CHS) in the common morning glory (
Ipomoea purpurea) comprise a multigene family, and they are divided into two subfamilies. The genes in a subfamily including the
CHS-A,
CHS-B and
CHS-C genes are distantly related to the other known
CHS sequences in a phylogenetic tree, whereas the
CHS-D and
CHS-E genes in another subfamily are more closely related to the well-characterized
CHS genes.
As an initial step to elucidate biological function of these
CHS genes in
I. purpurea, the
CHS-D and
CHS-E cDNAs were expressed in
Escherichia coli with different expression systems. The recombinant CHS-D and CHS-E proteins both showed CHS activity to produce naringenin chalcone. These results are discussed with regard to the biological roles of the
CHS-D and
CHS-E genes in flower pigmentation in
I. purpurea. We have also discussed these CHS-D and CHS-E enzyme as members of plant specific polyketide synthases.
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