A three-dimensional (3-D) analysis technique was developed to visualize the whole nodule and Nitrogen fixation (N
2-fix) zone in a stem nodule from Sesbania rostrata infected with symbiotic rhizobium, Azorhizobium caulinodans ORS571. The reconstructed N
2-fix zone of the stem nodule was analyzed by arranging the ratio of opacity, by cutting at vertical and level direction, and by extraction in a personal computer. The N
2-fix zone was a ring shape, surrounding the vascular bundle. This is the first report that shows whole distribution of N
2-fix zone in S. rostrata stem nodule.
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