In order to know the effects of organic substances on the physical and chemical behaviors of iron in sea water, solubilization of iron from the precipitated ferric hydroxide in sea water, related to the phyto- and zoo-plankton, was studied. In comparison with the results obtained with the artificial sea water with and without plankton, it was observed that the organic substances originated from plankton played an important role to change the state of iron in sea water. Especially, in the case of sea water in which Artemia salina was concerned, unstable but soluble iron was formed.
A neutron activation analysis for the simultaneous determination of multielements in tobacco leaves is descrived. The method is based on high resolutionGe (Li) spectrometer coupled to a computerized data reduction system. By selecting a suitable irradiation-cooling-counting system 13 elements in tobacco leaves were quantitatively determined without any chemical separation. Most of the photopeaks employed in the analysis were found to be free of spectral interferences. The detection limits of the elements to be determined in tobacco leaves under the present irradiation and counting conditions were evaluated. Several elements showed somewhat higher fluctuation in tobacco leaves of different origin.
Many methods of pulmonary function study have been used, most of which have been used for so-called overall pulmonary function studies. With some of these methods, the patients were made to endure much pain. However, pulmonary function studies using radioisotope are one of the useful methods of studying regional pulmonary fuction without subjecting the patient to much pain. For these reasons, we decided to perform the regional pulmonary function study using133Xe, which proved to be very useful and important. In performing regional pulmonary function studies using133Xe, we used the spot sampling method with 2 or more scintillation probes and the dynamic study method using scintillation-camera computer on line system. The half time of133Xe wash-out curve is calculated on the regional lung area under both methods. To use the combination of the133Xe ventilation scan and the perfusion scan with131I-MAA made the patho-physiological analysis of the pulmonary function easier. We have found in our133Xe regional pulmonary function studies that the analysis of regional pulmonary function change is as necessary as the analysis of regional pulmonary anatomical change, which is performed with information from chest X-ray film. Both together provide much more information in evaluating the clinical state of pulmonary disease in details.
Human serum thyrotro pin (TSH) was measured by“Daiichi HTSH Radioimmunoassay Kit”and its usefulness was evaluated in 112 subjects with and without thyroid abnormalities. This kit is based on a principle of double antibody radioimmunoassay. In each assay, all standards and unknowns were assayed in duplicate. Standard curves we obtained were good for reproducibility. A coefficient of variation of the duplicate was 4.8%. The lower limit of sensitivity in our experience was always about 1μU/ml of serum and the range was about one to 500μ U/ml. The percent bound at the zero level (B0/T) and blank without double antiboby were about 40% and 0.7%, respectively. Six of the nine patients in euthyroid group and all of the fourteen in hyperthyroid group had TSH values less than about 1μ U/ml of serum, which was the lower limit of sensitivity. The range and mean of values for serum TSH of euthyroid subjects were<0.5 to 2.8, and 0.8μU/ml, respectively. The range of levels of TSH in sera of euthyroid in our laboratory was similar to those reported by Kumahara, Jerome and Patel, using a Human Thyrotropin Research Standard A. We compared serum TSH with Res-O-Mat T4, T7and Triosorb, their correlation coefficients were -0.36, -0.30 and -0.15, respectively. The former two are significant at the 1% level. We further studied on the effect of thyrotro pin releasing hormone (TRH) to the subjects, which was called, “TRH test”. Injection of synthetic TRH (500μg) intravenously caused marked elevation of serum TSH in primary hypothyroid and euthyroid group, but not in hyperthyroid group. The peak of serum TSH was attained within 30 to 45 minutes after injection.