RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Volume 25 , Issue 10
Showing 1-17 articles out of 17 articles from the selected issue
  • Nobuhiro NONAKA, Kaneaki SATO, Hideo HIGUCHI, Hiroshi HAMAGUCHI
    1976 Volume 25 Issue 10 Pages 599-602
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    A rapid method for the determination of 137Cs in environmental samples was proposed. The principal technic employed in this study is based on column separation of 137Cs using ammonium molybdophosphate mixed with glass fiber to eliminate contribution of natural radionuclides such as 40K and 87Rb. The separation of cesium from potassium and rubidium was performed by the elution with 0.5M ammonium nitrate solution.
    The time required for separation of cesium was five hours as compared with the conventional cation exchange separation which required thirteen hours.
    The chemical yield of cesium carrier was normally more than 90 percent.
    The results obtained were compared with that by the conventional methods using Bio-Rex cation exchange separation and the good agreement between the two methods was obtained.
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  • Akira MURANAKA, Yasuhiko ITO, Tsuneji ICHIKAWA, Yoshinari IMAJO, Tsune ...
    1976 Volume 25 Issue 10 Pages 603-607
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    For the purpose to conduct precise geometry-independent radioassay of large biological samples, the authors constructed an apparatus with the “peak+scatter” counting method slightly modified from that of Gibbs, et al. and studied its accuracy from the basic and clinical aspects.
    The apparatus, an opposed-head counter, consisted of two matched 2×2-in. NaI crystal detectors and photomulti pliers that were connected in parallel to a pulse-height spectrometer. The crystal faces were separated by 54 cm. The detectors were enclosed in 5cm-thick shield built with lead and the sample chamber was a 15 cubic cm-box type of 3 mm-thick Lucite. A 13 cmφ×7.7 cm styrene topper container was used for the sample container.
    It was found that the relation of primary gamma energy of six radioisotopes and geometry-independent counting threshold turned out to be linear. The assay accuracy with 131I was about ±5% in fluid samples irrespective of its sizes (up to 500 ml) or homogeneity. In solid samples the accuracy approached that of fluid by the addition of water up to the height of it. The range, in which the activity could be measured within 5% error originating from statistical fluctuations or loss of counts due to instrument dead time, proved to be about 34-0.06μCi with 57Co in assay time of 10 min.
    On the basis of the basic investigations, the geometry-independent counting was used for the radioassay of stool and urine. Since the assay could be done simply up to a relatively large volume without need of material adjustment, it served well in the assay of urine and stool of clinical practice as well as in animal radioassay.
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  • Hisashi BUNKO, Kinichi HISADA
    1976 Volume 25 Issue 10 Pages 608-613
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Although recent progress in radioimmunoassay (RIA) permitted direct RIA for thyroxine (T4), there appeared few reports evaluating clinical applicability of T4 RIA as routine method. The authors evaluated advantage, disadvantage of methods for T4 measurement in comparison between CPBA (competitive protein binding analysis) and RIA, and tried to discuss about selection of methods for T4 measurement.
    Res-O-Mat T4 was chosen as CPBA, and RIAMAT-T4 (St) and T4-RIAKIT (Sp) were chosen as RIAs. Detailed descriptions about both RIA methods appeared elsewhere [2, 3] . From the basic experiments, reproducibility of CPBA, St and Sp within assay were 5.8% (C.V.), 4.0% (C.V.), and 8.5% (C.V.) respectively, and mean recovery rates were 92.2%, 100.7% and 96.1% respectively. T4 value measured by both RIAs correlated well with CPBA, and St showed slightly lower value and Sp showed slightly higher value than CPBA. The normal ranges for RIAs were 3.7-11.7μg% (St) and 4.6-15.2μg% (Sp) . Diagnostic accuracy for hyperthyroid state were 83.3% (CPBA), 85.7% (St) and 69.2% (Sp), and for euthyroid state were 92.0% (CPBA), 98.8% (St) and 95.7% (Sp) . The diagnostic accuracy for hypothyroid state in three methods were lowest. From the economical point of view, cost performance, time performance and total time for single assay were analysed and mathematical equations were obtained from actual assay by each methods. When the number of samples per single assay was less than 10, CPBA was most economic in terms of cost and time performance and total time required. In the assay of more than 20 samples, RIAs were efficient in each point of views. In the point of view of sample volumes required, RIAs were most promising.
    The authors concluded that guides for selection of methods in T4 assay were as follows: A) CPBA was most efficient in local use especially when less than 10 samples were frequently to be assayed. B) RIAs were efficient in batch processing more than 20 samples, especially in automated laboratories.
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  • Kazuo ITOH, Shin KOBAYASHI, Kinichi HISADA, Atsushi ANDO, Norihisa TON ...
    1976 Volume 25 Issue 10 Pages 614-621
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    This paper describes biologic distributions, sequential images and macroautoradiograms of 99mTcO4 (pertechnetate) , 99mTc-Sn-HSA (human serum albumin) and 99mTc-Sn-TSL (trasylol) in tumor-bearing mice as the first report on tumor affinity of 99mTc-labeled radio pharmaceutical.
    (1) Maximum tumor concentration (% administered dose/g of tissue weight) of 99mTcO4, 99mTc-HSA and 99mTc-TSL in Ehrlich's tumor-bearing mice resulted in 2.03±0.57 at 1 hr, 4.02±0.19 at 3 hr and 1.97±0.31 at 1 hr respectively.
    (2) However, tumor to blood concentration ratio of 99mTc-HSA was lowest among them.
    (3) The corrected tumor accumulation (% 100g dose/g of tissue wt. =% dose/% body weight) of 99mTC-TSL to Ehrlich's tumor in mouse was not different from that of Yoshida's sarcoma in rat, on the contrary to our expectation that the tumor concentration of 99mTc-TSL in them might be different due to diff erency of the tissue fibrinolytic activity between the respective tumors.
    (4) Sequential images of the implanted tumor in mouse was best positively delineated with 99mTc-HSA.
    (5) Macroautoradiograms of Ehrlich's tumor with 99mTcO4, 99mTc-HSA and 99mTc-TSL demonstrated the following findings: all of them were not only accumulated markedly into the tumor cells which were shown as basophilic tissue with Hämatoxylin-Eosin staining but also accumulated around the tumor tissue and on the interstitial tissue which were stained as eosinophilic tissue with the above same staining.
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  • Kazuo ITOH, Shin KOBAYASHI, Kinichi HISADA, Norihisa TONAMI, Atsushi A ...
    1976 Volume 25 Issue 10 Pages 622-629
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    The authors have examined the tumor affinity of various 99mTc-labelled radio pharma ceuticals to Ehrlieh's tumor for the purpose of delineating positively human malignant neoplasm. This paper includes biologic distributions of 99mTc-Sn-diphosphonate (99mTc-EHDP), 99mTc-Sn-dimercaptosuccinic acid (99mTc-DMSA) and 99mTc-Sn-diethyl stilbestrol diphosphate (99mTc-DSDP, 99mTc-Honvan) as the second report on the tumor affinity to the Ehrlich-bearing mice.
    (a) Tumor concentration of 99mTc-EHDP was lowest and the positive delineation of implanted tumor with 99mTc-EHDP was poorest in sequential images, though the active accumulation to some soft tissue maglinant neoplasms, the breast cancer and the thyroid cancer, has been reported.
    (b) Tumor concentration and tumor to blood ratio of 99mTc-DMSA were not so high on the contrary of our expectation that 197Hg-DMSA may show the high tumor concent-ration and the high tumor to blood ratio like 197Hg ehlormerodrin as same renal scanning radio pharmaceuticals.
    (c) Tumor concentration of 99mTc-DSDP was highest. Tumor to blood concentration ratio, however, was lower than that of the above mentioned radiopharmaceuticals but tumor to liver ratio and/or tumor to lung ratio was over 1.0 at the earlier time. Biologic distribution of 99mTc-DSDP was similar to that of 32P labeled DSDP and then it is presumed that 99mTc is labeled at phosphate ester of DSDP which is dephospholytated immediately by phospholylase in vivo following the intravenous injection. Therefore, it may be assumed that the accumulation mechanism of 99mTc-DSDP to Ehrlich's tumor is related to the phospholylase activity in neoplasms but is not known precisely.
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  • Atsushi ANDO, Kinichi HISADA, Itsuko ANDO
    1976 Volume 25 Issue 10 Pages 630-636
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    In order to investigate the tumor affinity radioisotopes, chromium (51Cr), molybdenum (99Mo), tungsten (181W), selenium (75Se) and tellurium (127mTe) -the elements of group VI in the periodic table-were examined, using the rats which were subcutaneously transplanted with Yoshida sarcoma. Seven preparations, sodium chromate (Na251CrO4), chromium chloride (51CrCl3), normal ammonium molybdate ( (NH4) 299MoO4), sodium tungstate (Na2181WO4), sodium selenate (Na275SeO4), sodium selenite (Na275SeO3) and tellurous acid (H2127mTeO3) were injected intravenously to each group of tumor bearing rats. These rats were sacrificed at various periods after injection of each preparation: 3 hours, 24 hours and 48 hours in all preparations. The radioaetivities of the tumor, blood, muscle, liver, kidney and spleen were measured by a well-type scintillation counter, and retention values (in every tissue including the tumor) were calculated in percent of administered dose per g-tissue weight.
    All of seven preparations did not have any affinity for malignant tumor. Na251CrO4 and H2127mTeO3 had some affinity for the kidneys, and Na275SeO3 had some affinity for the liver. Na2181WO4 and (NH4) 299MoO4 disappeared very rapidly from the blood and soft tissue, and about seventy-five percent of radioactivity was excreted in urine within first 3 hours.
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  • Susumu NAKAMURA, Ryuichi ISHIDA, Juko SUGIHARA
    1976 Volume 25 Issue 10 Pages 637-640
    Published: October 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    The Penfluridol (PFL) is a potent and long-acting neuroleptic belonging to the diphenylbutyl piperidine series.
    The purpose of the present study is to investigate a correlation between the brain concentration of unchanged 3H-PFL and its neuroleptic activity in the methamphetamine-antagonism test.
    The experimental animal comprised adult male Wistar rats weighing about 200 g. All the rats were administered 3H-PFL orally and killed at appropriate times after administration.
    After killing blood samples were collected and the brain and liver were excised. The blood was extracted with 4 volumes of ethanol. The brain and the liver were homogenized in 9 volumes of ethanol.
    The amount of 3H-PFL in the ethanol extract was determined by thin-layer chromatography.
    The result showed that the drug level in the brain was relatively constant when the dose was the median effective dose (ED50) for the test at those time intervals after administration.
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  • Toshio SHIGEMATSU, Kiyoshi KUDO
    1976 Volume 25 Issue 10 Pages 641-643
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Michiko ABE, Siro ABE, Nagao IKEDA
    1976 Volume 25 Issue 10 Pages 644-646
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Masakazu TANASE, Eiji SHIKATA
    1976 Volume 25 Issue 10 Pages 647-650
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Isao IKEDA, Osamu INOUE, Tomoyuki YAMAGUCHI, Kunio KURATA
    1976 Volume 25 Issue 10 Pages 651-653
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Norihisa TONAMI, Kyoichi UENO, Kinichi HISADA, Masamichi MATSUDAIRA, K ...
    1976 Volume 25 Issue 10 Pages 654-655
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Keisuke KANAO, Shizumori ISHIHARA, Yumiko Toe, Etsuko YAGI, Yoshihiko ...
    1976 Volume 25 Issue 10 Pages 656-658
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Osamu MATSUOKA
    1976 Volume 25 Issue 10 Pages 659-669
    Published: October 15, 1976
    Released: July 21, 2010
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  • Committee on Test of Transport Packaging for
    1976 Volume 25 Issue 10 Pages 670-675
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Eitaro WADA
    1976 Volume 25 Issue 10 Pages 676-685
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • 1976 Volume 25 Issue 10 Pages A1667-A1218
    Published: October 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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