RADIOISOTOPES
Online ISSN : 1884-4111
Print ISSN : 0033-8303
ISSN-L : 0033-8303
Volume 25 , Issue 12
Showing 1-16 articles out of 16 articles from the selected issue
  • Masahiko FUJITA, Kiyoshi IWASHIMA, Eigo TAKABATAKE, Noboru YAMAGATA
    1976 Volume 25 Issue 12 Pages 765-768
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    A new method has been devised for determining mercury and arsenic simultaneously in biological materials. It is based on complete digestion of the irradiated samples on a hot-plate, extracting arsenic as arsenic (III) chloride with benzene and isolating mercury by reductive aeration with tin (II) chloride. These elements are precipitated as sulfides and the activities are counted for quantitative evaluation. The chemical yield is determined by the use of 74As- and 203Hg-spikes and the neutron flux is checked by the use of copper as a flux monitor. The detection limits are 0.5 ng of As with counting error of ±15% and 1 ng of Hg with ±20%. The method was applied to the determination of mercury and arsenic in the maternal and neonatal hair and blood.
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  • Sigeaki MATSUMOTO, Hisanobu KOBAYASHI
    1976 Volume 25 Issue 12 Pages 769-772
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    A new method of determining the melting points was devised using the principle that the β-rays backscattered by the organic compounds placed on the heated metal disk increase rapidly at the melting point because the scattering area of the compounds becomes small by the effect of capillary action in the melting and the metal disk surface covered with the compounds is partly exposed. Carbon-14 and platinum plate were used as the β-ray source and the disk, respectively. The measured melting points of powdered azobenzene, sulfanilamide, dicyandiamide and phenol phthalein agreed with the correct melting points within an accuracy of ±2°C. The quantities of sample necessary for measurements are about 1 mg. This method is free from error due to visual observation and is also free from the β-ray source contamination.
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  • Hiroshige MORISHIMA, Taeko KOGA, Hiroshi KAWAI, Yoshihide HONDA, Kosuk ...
    1976 Volume 25 Issue 12 Pages 773-778
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Studies on the accumulation and distribution of uranium by some vegetables were made under pot cultivation using the sandy soil of high level uranium sampled at Muro village, Nara prefecture.
    The following results were obtained;
    1) The biological absorption ratios of uranium in cotyledon and upper leaves of radish were higher than the other parts. However, these were influenced by the contents of plant nutrient elements in the soil. The absorption of uranium by radish under the cultivation with deficient fertilizer was higher than that with three elements (N, P, K) fertilizer. The distribution of uranium in radish was nearly proportional to the ratio of ash weight. The distribution of uranium in pimiento and cucumber were also similar to that in radish.
    2) The uranium contents in the leaves of radish increased slightly and those in the roots decreased as growth of the plant.
    3) A positive correlation between the uranium contents in soil and those in the leaves of vegetables was observed under the soil of similar characteristics and different uranium levels.
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  • Tadashige TAKAHASHI, Susumu HAYAKAWA, Atsushi TAKAGI, Yoshihiko SUGISA ...
    1976 Volume 25 Issue 12 Pages 779-785
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Since Chopra, et al. produced antibody of triiodothyronine (T3), measurement of serum T3 level by radioimmunoassay has been widely done as a clinical test. However, dextran coated charcoal (DCC) method which is most commonly used for B/F separation requires strict control of incubation temperature and time. The authors improved this disadvantage by using polyethylene glycol (PEG) instead of DCC for B/F separation. Crossreactivities of used antibody with thyroxine, monoiodotyrosine and diiodotyrosine are less than 0.06% which is considered to be satisfactory in comparison with those previously reported.
    The recovery ratios of added T3 were between 92.4-105.9% and that reflected T3 concentration well. The dilution test showed good linearity. Correlation of T3 values measured by DCC method and PEG method in 26 sera was good (r=0.99) and linear regression function was y=0.89x+0.30 (y: PEG method and x: DCC method) .
    No significant difference were observed in the results obtained by 2 hr incubation at 20-35°C and 24 hr incubation at 4°C. As for the volume of PEG solution, 1.0 ml was most suitable. Samples should be kept at 4°C when centrifugation is impossible within 30 min after addition of PEG.
    Also in various conditions of centrifugation, the authors obtained almost same results.
    As described above, the authors conquered problem involved in DCC method, and also simplified the assay procedure by using PEG method for B/F separation. PEG method is considered to be a method of choice for clinical routine test for serum T3 levels.
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  • Kazuo ITOH, Shin KOBAYASHI, Kinichi HISADA, Norihisa TONAMI, Atsushi A ...
    1976 Volume 25 Issue 12 Pages 786-793
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Biologic distribution of 99mTc-labeled anti-neoplasm ic radiopharmaceuticals, 99mTc-L-asparaginase and 99mTc-bleomycin, in Ehrlich's tumor-bearing mice have been studied in order to get a promising indicator for the positive delineation of malignant tumor. The preparation of 99mTc-labeled radiopharmaceuticals, 99mTc-LASP and 99mTc-BLM, was made by the reduction with the stannous chloride and labeling efficiency was examined by gel chromatography and by silica gel plate thin layer chromatography. Labeling yields of 99mTc-LASP by Sephadex G-25M and of 99mTc-BLM by Bio Gel P-10 100-200 mesh was 82% and 62% respectively. A higher uptake of 99mTc-LASP was found with regard to the absolute tumor tissue concentration in tumor-bearing mouse. However, a slower blood disappearance and high uptake for liver and kidney was found in the biologic behavior of 99mTc-LASP. On the contrary, a lower tumor tissue uptake, a fast blood disappearance and a low concentration in different organs were found in the dynamic in vivo distribution of 99mTc-BLM. If it would be proposed for 99mTc-labeled tumor scintigraphic radiopharmaceuticals as a promising indicator for the positive delineation of malignant solid tumors that 99mTc-labeled compounds possess, of course, a high tumor uptake and, at least, a fast blood disappearance with a low uptake for different organs at the early time, 99mTc-BLM may be more preferable as 99mTc-labeled tumor localizing radiopharmaceuticals than 99mTc-LASP.
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  • Hisashi BUNKO, Norihisa TONAMI, Kinichi HISADA
    1976 Volume 25 Issue 12 Pages 794-799
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Recently many articles according to usefulness of 201Tl myocardial scan have appeared after early investigation by Lebowitz, Bradley-Moore and others. The authors had opportunity to evaluate 201Tl as myocardial scanning agent and studied its scanning condition and early organ accumulation up to 10 minutes after intravenous injection with large field of view gamma camera (Toshiba GCA-401) in order to obtain basic information for 201Tl myocardial scan.
    The study with liver slice phantom showed best results in 80 keV photopeak with 20% window width in low energy collimator. Normal myocardium (NM) and lung activity decreased rapidly to reach plateau within 3 minutes after injection. Activity in NM and liver changed little after 5 minutes postinjection, but infareted myocardium (MI) and lung activity still continued to decrease. In each case MI always showed low activity compared to normal area of the myocardium, and mean MI/NM ratio distributed from 0.74 to 0.80 up to 10 minutes postinjection. Blood disappearance of 201Tl was two exponential: 2.5 minutes (rapid phase) and 54.7 minutes (slow phase) . Effective half life of 201Tl in the whole body measured by linear scan was 2.22±0.46 days, and whole body radiation dose was thought to be about 160 mrads/mCi.
    201Tl myocardial scan taken from 5 minutes after injection delineated MI as a cleararea of decreased activity. Kidney showed highest activity, and NM showed same as and/or slightly greater than liver and spleen activity. Mediastinal activity always showed lowest activity, resulting good contrast myocardial scan. From these results, time to begin myocardial scan could be as early as 5 minutes after injection.
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  • Atsushi ANDO, Kinichi HISADA, Itsuko ANDO, Sigeru SANADA, Tatsunosuke ...
    1976 Volume 25 Issue 12 Pages 800-804
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    In order to investigate the tumor affinity of 99mTc-citrate complex, 99mTc-citrate complex was synthesized by three different ways. 99mTc-citrate complex was synthesized either by (1) reducing 99mTcO4- with FeCl3-ascorbic acid in 0.15 M sodium citrate, (2) reducing 99mTcO4- with SnCl2 solution in 0.15M sodium citrate or (3) reducing 99mTcO4- with NaBH4 in 0.45 sodium citrate. It was presumed by thinlayer chromatography that 99mTc-citrate complexes synthesized by three ways were chemically different each other.
    These 99mTc-citrate complexes were injected intravenously to the rats subcutaneously transplanted Yoshida sarcoma and these rats were sacrificed at one hour and three hours after injection. The radioactivities of the tumor, blood, muscle, liver, kidney, spleen and urine were measured by well-type scintillation counter. The retention values in these organs and the excretion rates in the urine were calculated. 99mTc-citrate complex synthesized by reducing 99mTcO4- with SnCl2 solution had considerably strong affinity to the malignant tumor but other two 99mTc-citrate complexes had not affinity to the malignant tumor. Excretion rates (% dose) of 99mTc-citrate complexes in one hour were from 65% to 75%.
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  • Teruo FUKUDA, Kunio HAMADA, Hironobu OCHI
    1976 Volume 25 Issue 12 Pages 805-810
    Published: December 15, 1976
    Released: September 07, 2010
    JOURNALS FREE ACCESS
    Animal studies and clinical studies of 99mTc-EHDP were done and the diagnostic efficiency of this reagent was discussed.
    Organ distribution and blood clearance of 99mTc-EHDP were studied in rats. 41.5% of administrered dose was accumulated in the skeleton at 3 hours and 46.2% at 5 hours after injection. Blood clearance of this compound was rapid, with 0.37% of retention in blood at 3 hours and 0.22% at 5 hours after injection.
    Comparative study of organ distribution and blood clearance after injection of 99mTc-EHDP, 99mTc-pyrophosphate, and 87mSr was done in rabbits. Blood clearance curve consisted of 2, 3 components in each of these reagents. 99mTc-EHDP showed the most rapid clearance followed by 99mTc-pyrophosphate, and 87mSr showed the latest clearance. In each o f bone/blood, bone/muscle, bone/liver, bone/kidney ratio, 99mTc-EHDP showed high ratio suggesting that bone scintigrams with this reagent are clear. In clinical study as well, blood clearance of 99mTc-EHDP was faster than that of 99mTc-pyrophosphate, and 29.5% of administered dose of 99mTc-EHDP was excreted in urine at 3 hours after injection.
    Scintigraphic evaluation of 99mTc-EHDP was done in 61 patients. Scintigrams were excellent in 54 patients (89%), good in 5 patients (8.2%), and not good in 2 patients. In these 2 cases, the physical condition and renal function were severe.
    For these reasons, 99mTc-EHDP was considered to be a hopeful reagent for bone scintigraphy.
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  • Takashi SUZUKI
    1976 Volume 25 Issue 12 Pages 811-813
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Masataka HIGUCHI, Akiko MUKADE
    1976 Volume 25 Issue 12 Pages 814-817
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Kazuro KAISE, Katumi YOSHIDA, Shintaro SAITO
    1976 Volume 25 Issue 12 Pages 818-821
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Tatsuya MIYAMAE, Masayoshi AKISADA, Yoshihiro INOUE
    1976 Volume 25 Issue 12 Pages 822-824
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Machiko KUMANO, Kazuyuki NARABAYASHI, Tomoho MAEDA
    1976 Volume 25 Issue 12 Pages 825-828
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Norihisa TONAMI, Takatoshi MICHIGISHI, Hisashi BUNKO, Masami SUGIHARA, ...
    1976 Volume 25 Issue 12 Pages 829-831
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • Shigeo BABA
    1976 Volume 25 Issue 12 Pages 832-841
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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  • 1976 Volume 25 Issue 12 Pages A1683-A1234
    Published: December 15, 1976
    Released: July 21, 2010
    JOURNALS FREE ACCESS
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