RuNO-Chelex 100 resin, [R·RuNO (OH) (OH2) ] was prepared by the reaction of RuNO-chloro complex with Chelex 100 resin, and it is expected that aqua and hydroxo groups contained in RuNO-Chelex 100 resin act as adsorption sites for glycinate or alaninate anion by ligand-exchange reaction. The equilibrium constants, Keq, of 1: 1 complex formation reactions of RuNO-Chelex 100 resin with glycine and alanine were found to be 96.3±5.76 and 82.6± 2. 69, respectively. The inhibitive effect of glycine and alanine on adsorption of (RuO2 (NO2) - (NO3) (H2O) NO) -to anion exchange resin increased in the order of glycine alanine. It was observed that the inhibitive effect was dominated by Keqof RuNO-Chelex 100 resin with glycine and alanine.
The urinary aldosterone (ALD) was measured by aldosterone RIA kit and the following results were obtained. (1) We have known that ten percent solution of bovine serum albumin (BSA) instead of free serum ALD is used as the diluent of the urine in aldosterone RIA kit. (2) The upper limits of free ALD, HCl-ALD and β-glucuronidase ALD in the urine diluted with 10% BSA were 1.6, 9.0 and 9.5μg/dl respectively. (3) The values of urinary conjugated HCl-ALD and R-glucuronidase ALD were approximately 3.5 times and 4 times as much as that of free ALD respectively. (4) A good correlation was obtained among the results of three methods (HCl-ALD, βglucuronidase ALD and free ALD) . (5) No difference was found in the values of the urinary ALD in the healthy subjects and the patients with essential hypertension, kidney diseases and acute liver diseases.
Plasma concentration of progesterone (P), 17a-hydroxyprogesterone (17a-P), 11-desoxy-cortisol (S), cortisol (F), 11-desoxycorticosterone (DOG), corticosterone (B), aldosterone (ALD), dehydroepiandrosterone free (DHA f ), dehydroepiandrosterone-sulfate (DHA-s) and testosterone (TEST) were measured in 26 essential hypertension (E.H.) and 6 primary aldosteronism (P.A.) before, 30 min. and 60 min. after the intravenous infusion of ACTH 250 fig. Seven E.H. patients were similarly studied in the condition of low and high sodium intake and plasma renin activity (PRA) was also measured at the same time. P.A. showed significantly higher ALD than that of E.H., whereas DHA f and DHA-s levels of these patients were lower than that of E.H. In men with E.H., TEST level showed step down response to ACTH. Low sodium intake induced a marked increase of ALD and PRA in E.H. In low sodium intake other mineralocorticoids such as DOG and B showed high value at 60 min (20% t value) after ACTH infusion. From these observations, it was suggested that the mineralocorticoids have a good response to ACTH in low sodium intake rather than high sodium intake, especially ALD has a cleary augmented response to ACTH. On the other hand, PRA showed only a reserve response to ACTH infusion.
Following its discovery by Edwards and Hayes (1969), the propensity of67Ga to accumulate preferentially in malignant tissues has been studied at diverse levels, not only for its importance in diagnostic imaging but also for its fundamental biological interest. The biodistribution of67Ga citrate seems to be strongly influenced by the concentration of serum iron and the unsaturated iron-binding capacity (UIBC) . Recently, it was found that the clearance of67Ga from blood and the attainment of high tumor to normal soft-tissue concentration ratios are accelerated if the serum iron concentration is high. The present investigation was undertaken in order to study the relationships between the change of the concentration of serum iron and67Ga accumulation in the tumor and soft-tissues in mice bearing Ehrlich's ascited tumor. The following results were obtained. (1) The uptake of67Ga in the tumor and soft-tissues except bone was decreased compared with controls if the concentration of serum iron was elevated by Fesin (saccharated ferric oxide) administered prior the injection of67Ga. (2) The urinary excretion of67Ga was considerably increased if the concentration of serum iron was elevated by Fesin administered prior the injection of67Ga. The results are consistent with the hypothesis that binding67Ga to transferrin is very important to the accumulation of67Ga in tumor and soft tissues.
Gallium-67concentrates in a variety of soft-tissue and tumor, however the accumulation is not specific for malignant tumor. The increase the tumor to non-tumor ratio is important to improve the quality of the tumor image. It has been recently found that the clearance of67Ga from blood and the attainment of high ratios of the tumor to normal soft-tissue concentrations are accelerated, if the serum iron concentration is high. The present investigation was, therefore, undertaken in order to study the effect of administered iron after injection of67Ga on the accumulation of67Ga in the tumor tissue of mice bearing Ehrlich's ascited tumor. The following results were obtained. The accumulations of67Ga in the tumor and soft-tissues were slightly decreased compared with controls after ferric acid saccharated (Fesin) administration 3, 6 and 24 hours after injection of67Ga. However, the tumor to blood ratio (T/B) in each group treated with Fesin was considerably increased because the clearance of67Ga from the blood is accelerated. The large tumor to blood ratio (T/B) would be advantageous for tumor scanning.