Simultaneous determination of32P and33P radioactivities was carried out with the liquid scintillation counter (Aloca LSC 670) . For the quenching correction, the channel ratio method was used, and the proper gain and window level were set to get the maximum performance number or Klein-Eisler value. Using this method the radioactivities of the32P and33P double labelled compounds which were separated with paper chromatography were determined. New index, RSA (relative specific activity, i.e.32P/33P) was proved to be effective as well as specific activity for the studies of the transport and metabolism of phosphorus compounds in the plant roots.
Sensitivity and precision of the13C-breath test were assessed by examining a couple of limiting factors caused by the sensitivity of the instrument used for13CO2analysis, endogenous fluctuation of13CO2abundance, and the residual CO2in sample storing tubes, vacutainers, etc. For13CO2analysis, a mass spectrometer equipped with a dual inlet, a dual collector, and an automated pressure matching system, was used. 15 ml vacutainers were used for sample storage. Endogenous fluctuation of13CO2abundance, however, was measured by putting the breath samples directly into the evacuated CO2purification system, instead of using vacutainers. Endogenous fluctuation (S.D.=0.202‰) was the most significant limiting factor, compared with instrumental limitation (0.085‰), or with influence of the residual CO2 (0.136‰) . Consequently, the sensitivity of the13C-breath test was figured out to be 0.52‰. This sensitivity is about 1, 000 times lower than that of14C-breath test. The precision was linearly dependent on13C increase from basal13C observed after administration of13C enriched compounds, Δ13C, and was determined to be expressed as 1.94 Δ13C.
On intravenous administration of201TlCl (74 MBq (2 mCi) ), initial transit of it through the heart as well as subsequent uptake by the chest were recorded in the supine position using a scintillation camera coupling to a small digital computer, and remaining the patient in the same position perfusion scintigram with99mTc-macroaggregated albumin (MAA) or transmission scintigram with a point source of99mTcO4-too. The radioactivity of total injected dose of201Tl (T) was calculated from the entire region on the initial transit of the tracer bolus through the heart, and that of unilateral lung (L) was done from the anterior image of201Tl on which isocount map of the perfusion image or transmission one was superimposed. By these procedures, the lung uptake ratio of201Tl was calculated by ratio of L/T, and expressed as percentage per entire unilateral lung and mean value (%) per pixel. The average values of the lung uptake ratio in circulatory diseases were 4.0 ± 1.2% in the right lung, 2.7±1.2% in the left lung, and in respiratory diseases 4.5±2.1% and 2.3±1.0%, respectively. This technique is useful to quantify the lung uptake of201Tl on the lung scintigram with201TlCl.
A multi-purpose gamma camera system which had 2 detectors of opposed position and was capable of radionuclide computed tomography (RCT) imaging was installed in Division of Nuclear Medicine, Chiba Cancer Center Hospital. In this study, the variance of RCT image due to the angular interval of sampling was analized. The full width at half maximum (FWHM) and full width at tenth maximum (FWTM) of point source were almost same for all sampling intervals investigated. The distortion of RCT images with uniform activity source could not be detected even for larger intervals. When the RCT images were obtained using a phantom contained 3 line sources and a bar phantom for RCT which were located at right angle with the plane of reconstruction, the images did not change between 1° and 6° of sampling interval, but for 9° or more the distortion of images occured explicity. For our system, sampling interval of 2° was predicted from the theorectical calculation considering both resolution and statistical noise. But, results from the phantom studies of present work showed that sampling interval of 6° was sufficient to reconstruct RCT images without distortion, and could be applicable to dynamic RCT images.