-
Hiromi YAMAKITA, Kiyoshi HAYAKAWA
1984 Volume 33 Issue 8 Pages
513-518
Published: August 15, 1984
Released on J-STAGE: September 07, 2010
JOURNAL
FREE ACCESS
Poly (methylvinylsiloxane) -bonded silica gel was synthesized by condensation reaction between silica gel and the hydrolysis product of methylvinyldichlorosilane. Graft polymerization of styrene or indene onto poly (methylvinylsiloxane) -bonded silica gel was carried out by γ-
ray irradiation and compared with that onto silica gel. Homopolymer was found to be formed through a radical mechanism concurrently with an ionic mechanism in the graft polymerization.
Probably genuine graft polymers are formed in the graft polymerization onto poly (methylvinylsiloxane) -bonded silica gel, while the product formed in the graft polymerization onto silica gel may be apparent graft polymers.
View full abstract
-
Toshihiko FUJII, Hisashi MIYAZAKI, Keiko NAMBU, Akira KAGEMOTO, Masahi ...
1984 Volume 33 Issue 8 Pages
519-525
Published: August 15, 1984
Released on J-STAGE: September 07, 2010
JOURNAL
FREE ACCESS
Dehydrocorydaline, an alkaloid from Corydalis bulbosa posessing anti-ulceric activity, was labeled by methyl-
14C
at position 13, and its disposition and metabolism were studied in mice and rats after oral (50 mg/kg) or intravenous (6.8 mg/kg) administration.
(1) Blood levels were as low as 0.5 (mice) and 0.06 (rats) μ
g eq./ml at maximum, conceivably due to its poor absorption from digestive tracts.
(2) Radiometric and autoradiographic studies after oral administration of 14C-
dehydrocorydaline revealed that appreciable radioactivity was distributed in rather restricted organs, namely, digestive tracts, liver and kidney. Comparison of distribution with that after intravenous administration suggested that the compound is subject to the significant first pass effect by the liver.
(3) The distribution pattern of radioactivity in the liver was heterogeneous regardless to administration routes.
(4) Radiometry and autoradiography also revealed that the disposition did not differ significantly from each other in normal rats and those with experimentally induced gastric ulcer.
(5) Excretion of radioactivity occurred largely in feces. Excretion via urine and bile was quite minor.
(6) Metabolite analysis suggested that dehydrocorydaline was metabolized by O-demethylation and subsequent conjugation with glucuronic acid. However, metabolites were minor relative to the unchanged compound.
View full abstract
-
Kazuhiro SHIBA, Hirofumi MORI, Kinichi HISADA
1984 Volume 33 Issue 8 Pages
526-532
Published: August 15, 1984
Released on J-STAGE: September 07, 2010
JOURNAL
FREE ACCESS
As tumor-seeking agents, glucose analogues, natural amino acids, synthetic nonmetabolized amino acids, and precursor of nucleic acids, etc., labeled with positron emitter, such as 11C
and 18F
have been recently investigated. However, there are very few reports concerning comparative study of tumor uptake and tissue distribution of these agents. This preliminary paper describes comparative distribution and whole-body autoradiography of these agents. 14C
labeled deoxy-2-fluoro-D-glucose (FDG), L-, DL-leucine, 1-aminocyclopentane carboxylic acid (ACPC) , α-
amino isobutyric acid (α-
AIB), and thymidine were intravenously injected through tail vein into separate groups of the experimental animals. As the experimental animals, the mice with Ehrlich tumor and the rats with Hepatoma AH109A were used.
Within 30 min after injection, FDG had the highest tumor uptake and tumor to tissue ratios, although FDG was inferior to ACPC and thymidine in related to tumor to heart, lung and brain ratios. However, the time course study indicated that tumor uptake of ACPC, α-
AIB and D-leucine increased with time, whereas those of other agents decreased with time or reached a plateau. Thus, at 120 min after injection, ACPC had the highest tumor uptake and tumor to tissue ratios, although ACPC was inferior to FDG in related to tumor to blood, liver and pancreas ratios. Autoradiogram of ACPC showed very clear tumor image as well as that of FDG. The above data suggest that synthetic nonmetabolized amino acids, such as ACPC may be promising as tumor-seeking agents, when used with a single photon emission computed tomography, while glucose analogue such as FDG, are the best tumor-seeking agent, when used with a positron emission computed tomography.
View full abstract
-
Hideaki YAMAMOTO, Itsuo TAGUCHI, Hideko USUKI, Tadao SENGAN, Kojiro KI ...
1984 Volume 33 Issue 8 Pages
533-538
Published: August 15, 1984
Released on J-STAGE: December 13, 2010
JOURNAL
FREE ACCESS
New method of quality control based on x-R control chart for radioimmunoassay (RIA) kit was investigated.
Conventional x-R control chart was hard to found variations in each RIA kits. But the new method, trend variation analysis of x-R control chart by moving average method, could detect variation among the different lot of RIA kits.
This method should be very useful to do a quality control in RIA kits.
View full abstract
-
Nobuhiko ITO, Masamichi IKEDA
1984 Volume 33 Issue 8 Pages
539-542
Published: August 15, 1984
Released on J-STAGE: September 07, 2010
JOURNAL
FREE ACCESS
The method for determining optimum conditions of a liquid scintillation counter (LSC) for lowlevel counting of tritium was studied. To compare the lower limit of detection, the figure of merit EM/√B was used. The measurement system is a LSC connected with a multichannel pulse-height analyzer (MCA) . Both of tritium spectra and background spectra were searched for the condition that maximizes the figure of merit. The optimum condition obtained by using the MCA was fitted for a single channel pulse-height analyzer of the LSC. The figure of merit obtained by this method was 20.6% greater than that obtained by the balance point method.
View full abstract
-
Kazuhiko FURUKAWA, Kohji HASUNUMA, Shin-ichi HATANAKA, Toshio HAYASHI
1984 Volume 33 Issue 8 Pages
543-546
Published: August 15, 1984
Released on J-STAGE: September 07, 2010
JOURNAL
FREE ACCESS
Absence of plasmid DNA sequences in non-tumorigenic (crown gall tumor) strains of Agrobacterium tumefaciens was confirmed by DNA-DNA hybridization between purified tumor-inducing (Ti) plasmid DNAs isolated from the progenitor tumorigenic strains and 3H-
labeled whole cell DNAs from tumorigenic strains and their non-tumorigenic derivatives. The genes controlling tumorigenicity in plant and utilizability of nopaline as their sole nitrogen source were confirmed to locate on Ti-plasmids.
View full abstract
-
Hikoyuki YAMAGUCHI, Atsushi TATARA
1984 Volume 33 Issue 8 Pages
547-548
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Hideki MURAKI, Kazuya TSUTSUI, Koji SATO, Masashi NAKAZAWA, Yoshihiro ...
1984 Volume 33 Issue 8 Pages
549-552
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Hideki MURAKI, Kazuya TSUTSUI, Koji SATO, Masashi NAKAZAWA, Yoshihiro ...
1984 Volume 33 Issue 8 Pages
553-556
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Takashi NOMURA, Toshiro SAKURADA, Katsumi YOSHIDA, Kazuro KAISE, Nobuk ...
1984 Volume 33 Issue 8 Pages
557-560
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Tadako KOUSAKA, Takashi MISAKI, Kanji KASAGI, Keigo ENDO, Junji KONISH ...
1984 Volume 33 Issue 8 Pages
561-564
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Takeshi INOUE, Katsuhiko MAYUMI, Takashi SUGIMOTO, Shohei KOKEI, Hiros ...
1984 Volume 33 Issue 8 Pages
565-568
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Kenji YOSHIHARA, Takashi OMORI, Tsutomu SEKINE
1984 Volume 33 Issue 8 Pages
569-578
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Subcommittee for Radioisotope in vitro Test
1984 Volume 33 Issue 8 Pages
579-589
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
Radioimmunoassay Research Society of Japan
1984 Volume 33 Issue 8 Pages
590-598
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
1984 Volume 33 Issue 8 Pages
599
Published: 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS
-
1984 Volume 33 Issue 8 Pages
A57P-A86M
Published: August 15, 1984
Released on J-STAGE: July 21, 2010
JOURNAL
FREE ACCESS