99mTc-DTPA-galactosyl human serum albumin (99mTc-GSA) is a new liver-imaging agent which binds to hepatic binding protein. This study evaluated the sensitivity of 99mTc-GSA kinetics and imaging anatomy to hepatic ischemia-reper-fusion injury of obstructive jaundiced rats. Regional hepatic ischemia was induced by clamping the left hepatic artery and the left portal vein for 45 min. The blood clearance and hepatic accumulation indices were obtained on the basis of the dynamic data. A significant difference of these indices was observed between the ischemic group and the control. Marked depression in hepatic99mTc-GSA uptake was observed in the ischemic group. In conclusion, 99mTc-GSA is useful for evaluating the hepatic ischemia reperfusion injury in rats with obstructive jaundice.