We performed an imaging analysis of 42K in Arabidopsis using real-time radioisotope imaging system(RRIS). First, we purified 42K from an 42Ar―42K generator. And then, we characterized RRIS performance by quantitatively determining 42K using standard series. As a result, the dynamic range for 42K was determined to be at least three orders of magnitude. Next, we evaluated the level of self-absorption in Arabidopsis organs by comparing the signal intensity detected using RRIS and the actual radioactivity detected by a gamma-counting method. There was no significant difference in detection efficiency between the thick bolt(stem) tissue and the thin leaf tissue. The reason for scarce self-absorption could be related to the relatively strong β ray emissions that have a maximum energy of 3525.4keV.
Beryllium-7(7Be) concentrations in surface air were measured at Niigata Institute of Technology(NIIT) located in Kashiwazaki City, from September 2008 through August 2011, to estimate the contribution of stratospheric ozone(O3) to surface O3 concentrations. Beryllium-7 has been well known as a tracer for the transport of stratospheric O3 to the troposphere. The 7Be concentrations were measured in 697 samples and compared to the highest value(daily maximum) of hourly O3 concentrations measured during the same period at the Kashiwazaki Air Quality Monitoring Station located about 3km north of NIIT. There was a significant correlation between 7Be and maximum O3 concentrations for each month from March to August, in which the highest correlation coefficient(γ=0.557) occurred in June. The contribution of stratospheric O3 to surface monthly average O3 for each month was calculated from linear regression intercept (b) and the monthly mean value of the daily maximum O3 cocentration (Ym) as (Ym-b)/Ym. Stratospheric O3 contributions greater than the mean(14%) were obtained from March to July, with a maximum of 29% recorded in June.
We investigated the influence on Shinjuku, Tokyo of the Fukushima Daiichi Nuclear Power Plant accident by pulse height distribution using measured data of monitoring post. As a result, the time variation of the artificial radioactive nuclides which reached Shinjuku-ku from the nuclear plant became clear.Acute rises of the air dose rate were seen 4 times between 15 and 16 March 2011, and they were brought by many artificial radioactive materials passing around the monitoring post, and some nuclides such as 131I which fell as slight dry deposition. Main cause of the air dose rate increase between 21 and 23 March was considered to be 131I,134Cs and 137Cs as wet deposition, brought by the rainfall in this period.
Positron emission tomography with appropriate radioligands can visualize alteration of various neurotransmitter systems in vivo in humans. Dysfunction of cholinergic system is closely related to dementia, and cholinestesterase inhibitors are used for the treatment of Alzheimer's disease and dementia with Lewy bodies. We developed radioligands, [11C]MP4A and [11C]MP4P, for measuring acetylcholinesterase activity in the human brain. With the use of these radioligands, the cholinergic pathophysiology of Alzheimer's disease, dementia with Lewy bodies, Parkinson's disease, progressive supranuclear palsy, corticobasal degeneration, and frontotemporal demetia have been elucidated. This technique also allows to measure therapeutic effects of cholinesterase inhibitors in the brain of patients with dementia.
In the last 40 years 13C-nuclear magnetic resonance(13C-NMR) spectroscopy has been used as the method for measuring brain metabolism with 13C-compound in vitro, ex vivo and in vivo. The hypothesis that metabolic trafficking between neurons and astrocytes exists in the brain has been supported by the researches using that method. It has been revealed by using non-invasive 13C-MRS method that metabolic trafficking is altered in neurological and psychiatric disease.