Review of Polarography
Online ISSN : 1884-7692
Print ISSN : 0034-6691
ISSN-L : 0034-6691
Volume 58, Issue 2
Displaying 1-11 of 11 articles from this issue
Preface (Foreword)
Award Review Article, 2011 Shikata International Medal
  • Shunichi Uchiyama
    2012 Volume 58 Issue 2 Pages 67-73
    Published: September 27, 2012
    Released on J-STAGE: November 08, 2012
    JOURNAL FREE ACCESS
    This article presents a brief review of rapid coulometry exploited by author's group. This rapid coulometric method has been realized by using the porous carbon felt electrode impregnated with an electrolyte. This method adapts a concentration-step method differing from potential-step method employed in conventional controlled potential electrolysis. The sample added to the center of the carbon felt electrode rapidly diffuses into the inner part of the felt accompanied by the electrode reaction, and the complete electrolysis of the added sample is complete below 10-20 s. The various compounds such as L-ascorbic acid, vitamin K3, hydrogen molecule and peroxide have been determined by this novel coulometric method which has been commercialized by three Japanese manufactures so far. Biocoulometric devices have been also developed by combining enzymatic reactions and rapid electrolysis, in which enzyme is dissolved in electrolyte or immobilized at the surface of the carbon felt electrode, and moreover, a flow biocoulometric cell has been fabricated for the determination of substrates contained in blood or urine samples.
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Award Integrated Research Paper Article, 2011 Shikata Medal
  • Hirosuke Tatsumi
    2012 Volume 58 Issue 2 Pages 75-82
    Published: September 27, 2012
    Released on J-STAGE: November 08, 2012
    JOURNAL FREE ACCESS
    Kinetics of the enzymatic hydrolysis of insoluble polysaccharides has been studied by using amperometric biosensors. The hydrolysis of starch, cellulose, chitin, and chitosan, catalyzed by glucoamylase, cellobiohydrolase, chitinase, and chitosanase, respectively, has been successfully monitored by direct and continuous measurements of their products in thick suspensions without any influence of turbidity. The dependence of the hydrolytic rates on the enzyme concentration as well as on the amount and specific surface area of polysaccharides can be explained by the rate equation based on the Langmuir adsorption of free enzymes onto the surface of polysaccharides.
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