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Volume 21, Issue 4
Displaying 1-17 of 17 articles from this issue
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[in Japanese]1980 Volume 21 Issue 4 Pages 441-443
Published: 1980
Released on J-STAGE: January 26, 2009
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Hiroshi HARA1980 Volume 21 Issue 4 Pages 444-450
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSWhether the injured hemopoietic inducing microenvironment (HIM) is a cause of aplastic anemia or not is improtant in bone marrow transplantion. When the defect in the injured HIM can not induce the proliferation and differentiation of pluripotent stem cells, it may not be expected that the transplanted stem cells differentiate from stem cells to matured cells.
Recently, we established the methods to estimate pluripotent hemopoietic precursors in vitro, using methylcellulose cell culture thechniques. Then, we assayed the precursors in bone marrow cells from the patients with aplastic anemia. The numbers of the precursors in the patients decreased in accordance with the clinical state in the disorder. The results suggested that the pancytopenia in the patients with aplastic anemia is not the results from the loss of ability in HIM inducing the differentiation of the precursors but also due to the deficiency in number of pluripotent stem cells.
In the present papers, we also described the cytotoxic effects of anti-lymphocyte globulin (ALG) on granulocytic precursors which is used to prevent or treat graft versus host reaction (GVHR).
Furthermore, we described the methods of cryopreservation of human bone marrow cells, especially erythropoietic precursors (BFU-E). Also, a case of malignant lymphoma who was treated with a administrat on of massive cytotoxic agents and received autologous cryopreserved bone marrow transplantation was reported. At last, the investigation on the viabil ty of granulocytic precursors in bone marrow cells from cadavers indicated that hemopoietic precursors in the bone marrow obtained within 5 hrs. after death could be used as a source of bone marrow cells in transplantation.View full abstractDownload PDF (524K) -
Hirotoshi SHIBATA, Tohru MASAOKA1980 Volume 21 Issue 4 Pages 472-478
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSNine cases of bone marrow transplantation have been performed since 1975 for the treatment on 6 cases of acute leukemia (ALL, 4. AML, 1. AMoL, 1.) and on each of aplastic anemia, chronic myelogenous leukemia and malignant lymphoma.
In earlier 6 cases no marrow engraftment was established, but in a case of aplastic anemia the complete hematological recovery of the host type marrow was noted on 60 days after the bone marrow transplantation and has persisted for 31 months.
In recent 3 cases marrow engraftment was confirmed by the analysis of chromosomes, but all of patients were died by severe infections such as fungal, viral septicemia and pneumocystis carinii pneumonia. The longest survival dutation after engraftment was 106 days for acutelymphocytic leukemia. Only grade I of GVH reaction was found in two of these 3 cases.View full abstractDownload PDF (669K) -
[in Japanese]1980 Volume 21 Issue 4 Pages 478-479
Published: 1980
Released on J-STAGE: January 26, 2009
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[in Japanese]1980 Volume 21 Issue 4 Pages 479-481
Published: 1980
Released on J-STAGE: January 26, 2009
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1980 Volume 21 Issue 4 Pages 481-483
Published: 1980
Released on J-STAGE: January 26, 2009
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—I. In vitro Release of β-TG in Platelet Aggregation—Chiyomi SATO, Atsuo IIZUKA, Takeshi NAGAO1980 Volume 21 Issue 4 Pages 484-490
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSRelease of a platelet specific protein, β-thromboglobulin (β-TG) was evaluated on various aggregating agents.
β-TG was assayed by radioimmunoassay (Radiochemical Center, Amersham). It was found that collection of blood samples with 3.8% sodium citrate, centrifugation (250 g) at room temperature or 4°C, standing the PRP at 37°C for 5 min. and agitation of PRP at 37°C with stirrer resulted only a minor release of β-TG, up to 42 ng/ml at most. It was also found that EDTA & theophylline could interrupt on-going β-TG release, and dilution of samples with 0.64% BSA in phosphate buffer was useful to estimate β-TG levels above 200 ng/ml.
Based on the above results, we performed ordinary platelet aggregation, using collagen (10 μg/ml), epinephrine (10-4 M), ADP (10-5 M) and ristocetin (1.5 mg/ml). The reactions were stopped serially with EDTA & theophylline, and β-TG levels of the samples were assayed. Collagen aggregation induced abrupt increase of β-TG at 2 min., and the peak was 9,800 ng/ml at 2.5∼4 min. In ADP aggregation, β-TG was released abruptly at 1.5 min., and reached the maximum level (10,200 ng/ml) at 4 min. RIPA also released β-TG, but the maximum level was about the half of the above two. In all four aggregation, the aggregation curves were closely similar to the pattern of β-TG released. In short, β-TG was not released significantly during the first phase of platelet aggregation, and β-TG was thougt to be released as a consequence of platelet activation due to interaction between collagen and platelets or between aggregated platelets. β-TG seems to be a useful tool to evaluate platelet release reaction in vitro, as well as in vivo.View full abstractDownload PDF (471K) -
Takashi HANADA, Yasuhiro YODA, Toshiro NAGASAWA, Kenjiro TANOUE, Tsuka ...1980 Volume 21 Issue 4 Pages 491-497
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSThis is to report results of coagulation study in a rare case of disseminated intravascular coagulation complicated in dissecting aortic aneurysm. A 64 year-old Japanese male, known case of dissecting aneurysm since May, 1976, was admitted in Octorber, 1978 for subcutaneous hemorrhage. On physical examination multiple purpura on both arms and a pulsating mass in the abdomen were noted. Aortogram showed a picture of dissecting thoracic and abdominal aortic aneurysm. Coagulation studies on admission revealed a platelet count of 9.8×104/cmm, prothrombin time of 11.7 seconds (control 10.1 seconds), activated partial thromboplastin time of 56.1 seconds (control 40.3 seconds), plasma fibrinogen level of 125 mg/dl, FDP of 40 μg/ml and antithrombin III of 27.3 mg/dl. Activities of plasmin and antiplasmin in the plasma were decreased. Platelet aggregation by adrenaline, ADP and collagen was normal. 51Cr-labelled platelet survival T1/2 was 2.0 days and 125I-fibrinogen, 1.5 days. A diagnosis of DIC associated with dissecting aneurysm was made and continuous intravenous heparin infusion 12,000 U/day was started. Resultingly the abnormalities in coagulation studies, 51Cr-platelet and 125I-fibrinogen survival apparently improved. Activities of coagulation factors V, VIII and IX, which were decreased before the heparin therapy, recovered to levels more than twice as much as those were on admission. The patient underwent aneurysmectomy and a Dacron graft was implanted. But he expired on the ninth post operative day.
On the basis of the clinical data as well as histological findings from surgical and autopsy material, abnormal activation of intrinsic factors by the histological change of the aorta was considered to be responsible for the cause of disseminated intravascular coagulation.View full abstractDownload PDF (557K) -
—Discussions on its Effectiveness and Mechanism—Takashi HOSHINO, Atsushi UCHIDA, Takayuki TAKAHASHI, Tetsuo HORIUCHI1980 Volume 21 Issue 4 Pages 498-505
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSTreatment with ceruloplasmin combined with glucocorticoid, anabolic steroids or folic acid was attempted to 5 patients with aplastic amenia. The therapy was at least effective in all cases, including 2 cases with marked efficiency, to improve anemia or to reduce the amount of blood transfusions, suggesting some recovery of erythroid proliferation. The pharmacological mechanism of the hematological improvement was thought to be related to an amelioration of iron utilization, which was interpreted by posttherapeutic increases in plasma iron disapperance rate, plasma iron turnover rate and iron utilization rate on ferrokinetics. Despite clinical improvements, colony count of CFU-C (colony forming unit culture) of the bone marrow cells remained at very low levels along with circulating granulocytopenia and thrombocytopenia even after the therapy, suggesting that cerulopasmin has less proliferative effects on the myeloid series. However, because of its beneficial effect on the erythroid series, treatment with cerulopasmin will be worthwhile to attempt in refractory aplastic anemia.View full abstractDownload PDF (636K) -
Hiroshi MOHRI, Hideo TERADA1980 Volume 21 Issue 4 Pages 506-512
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSOsmotic fragility test of erythrocytes by coil planet centrifuge was studied in 33 patients of iron deficiency anemia, 9 of polycythemia vera, 8 of hemolytic anemia (hereditary spherocytosis 5, autoimmune hemolytic anemia 2, paroxysmal nocturnal hemoglobinuria 1), 7 of acute leukemia, 6 of aplastic anemia, 6 of malignant lymphoma, 3 of multiple myeloma, and 3 of SLE.
In 35 healthy adults, lysis of erythrocytes began at 101.4±4.8 mOsM and completed at 59.1±2.6 mOsM.
In iron deficiency anemia osmotic fragility was within normal range. The peak of osmotic fragility curve (HMP) was shifted to the left with improvement of anemia.
In hemolytic anemia osmotic fragility of erythrocytes always increased. In autoimmune hemolytic anemia the good correlation between erythrocyte osmotic fragility and reticulocyte counts. Osmotic fragility tended to be normalized when their hemolytic signs and symptoms improved.
In acute leukemia osmotic fragility increased in pre-treated or relapsed stage and normalized in remission.
In SLE marked increase in osmotic fragility was found in active stage and returned to normal in inactive stage.
In multiple myeloma osmotic fragility showed remarkable increase.View full abstractDownload PDF (508K) -
Masataka OJIRO, Koichi YOTSUMOTO, Shigeru KAWAIDA, Mtitsuru TAKENOSHIT ...1980 Volume 21 Issue 4 Pages 513-519
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSBlood viscosity is clinically important as to the blood circulation and coagulation.
The effect of Dextran on the blood viscosity and the variation of the whole blood and plasma viscosity were measured before and after operation. The viscosity was measured by ‘VISCONIC’ (Tokyo Keiki Co.)
Dextran infusion decreased hamoconcentration and whole blood viscosity, experinentally and clinically, and a significant positive correlation was observed between the hematocrit and blood viscosity, but no significant correlation was noted between fibrinogen and whole blood viscosity.
The effect of Dextran on the blood viscosity was due not only to the hemoconcentration but also to the viscosity of red cells. Namely, red cell viscosity was measured after the incubation of red cells with Dextran or saline. Red cell viscosity after the incubation with Dextran, was clearly lower as compared with that after the incubation with saline.
According to electron microscopic examination, the morphology of the red cells after the incubaiton with Dextran was changed after the incubation with saline distinctly. Red cells became more smooth, after Dextran incubation and rough after saline incubation.
The variation of blood viscosity was investigated at the time of pre-operation, the postoperative days 1, 3 and 7. The whole blood viscosity was increased after the operation, especially on the postoperative 7 days, but plasma viscosity did not change.
From the above results, we postulate that postoperative high blood viscosity promotes the postoperative hypercoagulable state, and so the effect of Dextran on the blood viscosity inhibits the postoperative hypercoagulable state.View full abstractDownload PDF (823K) -
—With Emphasis on the Bone Marrow Biopsy by Jamshidi Needle—Kazumi SAMPI, Akinori ISHIHARA, Tetsuo NAKAJIMA, Tsuneto TSUCHIMOCHI, ...1980 Volume 21 Issue 4 Pages 520-525
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSSixty-one patients with malignant lymphoma were investigated for bone marrow involvement by only marrow aspiration initially, and subsequently bone marrow aspiration and Jamshidi bone marrow needle biopsy. Bone marrow involvement was found in 6/50 (12%) patients with RCS, 2/2 (100%) with lymphosarcoma, and 1/9 (11%) with Hodgkin's disease. Bone marrow was involved in one patient with CS II lymphosarcoma, 4/16 with CS III and 4/20 with stage IV. In total 5 of the 41 patients (12%) was found to have been down-staged. 42 procedures of bone marrow needle biopsy and bone marrow aspiration in the same patients and at the same time were performed. 9 procedures were found to be positive for bone marrow involvement by marrow needle biopsy while only 2 of these positive by bone marrow aspiration. Accordingly, it is suggested that marrow needle biopsy is superior to the bone marrow aspiration. Anemia and/or thrombocytopenia found in 6 of 9 patients with initial marrow involvement.View full abstractDownload PDF (553K)
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Takeo KUROYANAGI, Kazuo KURA, Toshio ARAO, Hirofumi NAGOYA1980 Volume 21 Issue 4 Pages 526-535
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESS57 y. o. male
Patient was quite well until he was administered to a hospital because of a trafic accident. During the hospital stay he was found to have the hepatosplenomegaly and diagnosed as Banti syndrome. The pathohistological examination on his exstirpated spleen revealed the presence of depositis of amyloid. He had the thyroid and adrenocortical hypofunction due to pituitary hypofunction. He had the λ type macroglobulinemia and the Bence-Jones proteinuria. The amyloid protein was stained specifically by FITC labeled anti-λ light chain antibodies. A marked decrease of IgG and IgA Sm-Ig bearing B cells and a marked increase of IgM Sm-Ig bearing B cells were demonstrated. A marked decrease of and Tγ cells and marked increase of B cells were demonstrated. The in vitro IgG and IgA production of B cells by the stimulation of PWM was markedly decreased but the IgM production was within normal limits. When patient's T cells and normal allogeneic B cells were mixed lymphocytes cultured and then B cells were stimulated by PWM, their production of IgG, IgA and IgM was markedly reduced, suggesting the increase of suppressor T cells in the patient. Patient's B cells were resistant to he regulation of their differentiation to IgM producing cells by normal activated suppressor T cells.View full abstractDownload PDF (1216K) -
Tomomitsu HOTTA, Noriyuki HIRABAYASHI, Tatsuya KOBAYASHI, Shigetoshi N ...1980 Volume 21 Issue 4 Pages 536-543
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESS5 patients with uncommon hematological damages following the administration of diphenyihydantoin (DPH) after carniotomy were reported.
Intravascular hemolysis accompanied by marked hemoglobinuria occurred in 5 patients who had received DPH for 1 to 3 weeks after craniotomy. Moreover, marrow aplasia developed in one patient and pure red cell aplasia in 2 among these 5 patients. The former died from acute renal failure 2 weeks after the onset of the symptoms. The others recovered shortly after the withdrawal of the drug. In addition to the hematological damages, mild hepatic dysfunction was observed simultaneously and/or prior to hemoglobinuria in all cases.
It was suggested that hematotoxicity of DPH appeared under the hepatic dysfunction due to halothane which had been used for the anesthesia at craniotomy.View full abstractDownload PDF (535K) -
Hoyu TAKAHASHI, Eiji ODA, Akira HATTORI, Isao KOBAYASHI, Kaoru TAKAHAS ...1980 Volume 21 Issue 4 Pages 544-554
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSTwo cases in a family with von Willebrand's disease which revealed an increased ristocetin-induced platelet aggregation (RIPA) and a qualitative abnormality of the factor VIII protein are herein reported. The proband was a 5 year old girl with prolonged bleeding time, factor VIII procoagulant activity (VIII: C) of 45∼54%, factor VIII related antigen (VIII R: AG) of 50∼110% and von Willebrand factor activity (VIII R: WF) of 18∼35%. Her father showed almost the same results as the proband in his hemostatic evaluation. The threshold concentration of ristocetin giving 30% increase in light transmission was 0.5 mg/ml in the proband and 0.4 mg/ml in her father (normals: 1.16±SD 0.18 mg/ml). The amount of VIII R: AG in their platelets were normal, but an increased binding affinity of platelets to plasma factor VIII was demonstrated.
The qualitative abnormality of the factor VIII protein was characterized by an increased anodal migration of VIII R: AG on crossed immunoelectrophoresis, a retarded elution pattern as demonstrated by gel filtration on Sepharose 2B and a reduced precipitation with concanavalin A. In both patients a recovery of VIII R: AG to the cryoprecipitate was also reduced.
The responses to factor VIII concentrates and DDAVP were also investigated.View full abstractDownload PDF (1468K) -
Tadako NAKATSUJI, Noboru MATSUMOTO, Susumu ODA, Kenji SHINOHARA, Shiro ...1980 Volume 21 Issue 4 Pages 555-561
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSA case of primary acquired sideroblastic anemia terminated in acute myeloblastic leukemia is reported. The patient was a 39-year-old female, who had been treated as aplastic anemia for three years at another hospital. Laboratory examinations on admission disclosed hypochromic anemia with occasional macrocytes, elevated serum iron, increased Hb F level (4.3%), reduced δ-aminolevulinic acid synthetase activity, erythroid hyperplasia with many ringed sideroblasts and atypical blast cells up to 17 per cent in the bone marrow. At this stage, leukemic transformation in the near future was highly suggested. A large dose of Pyridoxine, folic acid or anabolic steroid (oxymetholone) were not effective. After one year and six months following the diagnosis of sideroblastic anemia, marked proliferation of myeloblasts in the marrow with appearance of leukemic cells in the peripheral blood was noted. Combined chemotherapy was not beneficial to induce remission and she died from pneumonia. Autopsy revealed wide-spread leukemic cell infiltration and hemosiderin deposits in the liver, spleen, pancreas and stomach.View full abstractDownload PDF (1012K) -
Kenjiro TANOUE, Toshiro NAGASAWA, Yasuhiro YODA, Tsukasa ABE, Takeo NO ...1980 Volume 21 Issue 4 Pages 562-570
Published: 1980
Released on J-STAGE: January 26, 2009
JOURNAL RESTRICTED ACCESSThe mechanism of cyclic thrombocytopenia developing in a 36-year-old woman was studied. The distribution of platelet size differed depending on phases in the cycle, per cent macrothrombocytes being maximal at the lowest platelet count and decreasing as platelet counts rose. On the other hand, serial examinations of the bone marrow revealed neither megakaryocytic hypoplasia nor significant changes in the ploidy of megakaryocyte nuclei during the cycle. In addition, the platelet cycle was not accompanied with such a cyclic pattern of either peripheral reticulocytes or neutrophils. These observations suggested that the platelet cycle in this patient would be ascribable to variation in the destruction rate of platelets rather than their production rate, although the half survival of platelets was short as 0.8 days even at the peak platelet count. The accelerated platelet destruction was not related to periodic changes of the endometrium since hysterectomy did not result in disapperarance of the cyclic pattern of platelets. During our observation the periodicity of platelet counts was apparent when she received prednisolome, while sever thrombocytopenia persisted without administration of the corticosteroid.View full abstractDownload PDF (672K)
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