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Volume 31, Issue 6
Displaying 1-30 of 30 articles from this issue
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[in Japanese]1990 Volume 31 Issue 6 Pages 693-701
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (690K)
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Youichi AMEMIYA, Yoshiichi HASHIMOTO, Takashi OBATA, Yoko NAKAGI, Yasu ...1990 Volume 31 Issue 6 Pages 702-712
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSFCM has become an invaluable technique in a variety of clinical and research applications. Then, FCM is used for quantitative analysis of human erythrocyte antigens using Spectrum III.
Antibody sesitizations give rise to erythrocyte aggulutination making the FCM analysis of a single cell impossible. However, such agglutinatin could be prevented by some of erythrocyte fixations with PFA and DMS. To make examinations in an optimal consentration of primary antibodies was important for FCM assay.
FCM was used to detect small percentage of D antigen positive cell contaminated in negative samples, subsequently positive cells were finally detected in 0.2%. This assay was utilized on a recipient of M/N mismatched allogeneic BMT, and an accurate proof of engraftment was obtained.
Relationship of the numbers of antigen determinants and immunofluorescence intensities might be in linear correlation by log-log transformation. The fluorescence intensities of D antigen were analyzed for different rhesus phenotypes and variants such as Du and -D- cells. Lower fluorescence intensities on Du and higher intensities on -D- cell could be seen comparing with average value of D positive cells. However, no relationship between rhesus phenotypes and anti-D immunofluorescence intensities was observed.
These findings suggest that FCM is usefull and accurate technique for measuring relative antigen determinants of erythrocyte.View full abstractDownload PDF (737K) -
Kazuo TSUBAKI1990 Volume 31 Issue 6 Pages 713-719
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSResults obtained by analysis of anti-platelet antibody using flow cytometry (FCM) were as follows. The use of nest gating is useful in analysis of anti-platelet antibody and frequency of antibody production were 32.7% in patients with hematological disorders using FCM method. This method was much correlated to AHG-LCT method. The rate of coincidence was 92.1%. And effective rate of platelet transfusion of cross match carried out only by FCM method was 81.3%. Concentration of globulin fraction in patient's serum and avidin-biotin assay are useful for increasing the sensitivity of detection of anti-platelet antibody. Elimination of HLA (Class I) antigen from platelet's surface is effective in both chloroquine and acid treatment. Background of fluorescent intensity was tendency to low by the acid treatment than by chloroquine one.
It is possible that high titer serum of allogeneic anti-platelet specific antibody is able to be identified by means of its fluorescent intensity.View full abstractDownload PDF (598K) -
Kozo TAKASE1990 Volume 31 Issue 6 Pages 720-728
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe have evaluated the clinical efficacy and problematic items of flow cytometric surface and intracellular immunophenotyping of leukemic cells. The multi-parametric analysis on the results of leukemic phenotyping displayed a possibility of an independent phenotypical classification, but concurrently we recognized complementary relationships between the morphological and phenotypic approaches. Defective information on the morphology of leukemic cells unexpectedly introduced an insufficient quality in performance of leukemic phenotyping. On the other hand, the interpretations of phenotypic outcomes were complicated in cases with mixed leukemia and partial expression of a marker. Finally we hope further integration of the expression spectrum of markers, including the improvement of CD nomenclature system.View full abstractDownload PDF (786K) -
Shigeru TAKAMOTO, Hidefumi KATO1990 Volume 31 Issue 6 Pages 729-735
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSDNA aneuploidy (DA) was examined in adult leukemia using flow cytometry, and the method and the clinical implication of DA as a tumor marker were evaluated. The method was simple, rapid, objective, quantitative and further did not need any mitotic cells, so was proved to be very useful for screening of DA. While, DA was detected in 50 (27%) out of 185 adult cases with various types of leukemia. The frequencies of DA in the subtypes of leukemia were 55% in ATL, 26% in ALL, 17% in ANLL, 26% in CML-BC and 6% in CLL, respectively. When compared with other subtypes, the frequency in ATL was significantly higher (p<0.01), which suggested a special entity of this disease. In general, however, the frequency of DA in leukemia was rather low, which indicated the difficulty in application of DA by itself in diagnosis of leukemia. While, in cases with DA, DA was very useful as a tumor marker in monitoring the clinical course, for example, in the detection of early relapse or recruitment of leukemic cells. Furthermore, DA was found to be a good prognostic factor which indicates a poor prognosis in cases with ANLL and CML-BC.View full abstractDownload PDF (568K) -
Hiroyuki TSUCHIYA1990 Volume 31 Issue 6 Pages 736-748
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSFlow cytometry (FCM) is a useful method for clinical research of oncogene products since it can analyze proteins quantitatively which are located at cell surfaces or inside of cells. Oncogene products are now under study by FCM not only as tumor markers but also as functioning proteins in carcinogenesis. The examples of oncogene products analyzed by FCM are ras, myc, p 53, myb and fos; those of cell-proliferation-related proteins are Ki-67, PCNA and DNA polymerase α. In some diseases the relationship between these proteins and disease classification, stage, pathophysiology, or prognosis have been clarified.
Using dual color FCM of H-ras p21 and DNA, we analyzed the expression of H-ras p21 in human multiple myeloma and leukemias and found that H-ras p21 levels in multiple myeloma strongly correlated to the prognosis of patients (p=0.03). When AML cells were stimulated by adding G-CSF, it was found that many cells proliferated but some were dying. The percentage of dying cells was small in one AML case whose myeloblasts showed increased expression of H-ras p21 by G-CSF stimulation. Together with other papers reviewed, it is conceivable that H-ras p21 expression is related to cell proliferation and inhibition of cell autolysis. Thus FCM is useful in the clalification of the role of oncogene products in carcinogenesis in clinical cases.View full abstractDownload PDF (975K)
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[in Japanese], [in Japanese]1990 Volume 31 Issue 6 Pages 749
Published: 1990
Released on J-STAGE: March 12, 2009
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Hajime TSUJI1990 Volume 31 Issue 6 Pages 750-755
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSBiological properties and the efficacy of AT III concentrate for the treatment of thromboembolic disorders were evaluated in the patients with AT III deficiency.
Commercially available AT III concentrates showed heterogeneity on agarose gel isoelectric focusing, however, they inhibited thrombin in the same manner. At III concentrates were infused to 11 patients with congenital AT III deficiency (4 with thrombosis). Pharmacokinetic parameters of infused AT III were calculated as follows; half time 61 1±23.0 hr. (58.4±22.6 hr. in the cases with thrombosis), max increase rate 1.01±0.3%/U/kg and recovery rate 95.4±33.3% Simulation curves adjusted to the multiple administration were correlated well with the actually determined values in the patients and steady state concentration of AT III was achieved by the administration of this concentrate in 12 or 24 hour intervals. Clinically, substitution with AT III concentrate was proved to be effective for the treatment of thromboembolism in these patients. 16 patients with disseminated intravascular coagulation were treated with heparin (6,000 U/day) followed by AT III concentrate (1,500 U/day) administration. Clinical symptoms and laboratory findings were improved in 11 patients.
From these results substitution with AT III concentrate was suggested to be beneficial for the prevention or the treatment of thromboembolic disorders in the patients with AT III deficiency.View full abstractDownload PDF (468K) -
Hidesaku ASAKURA1990 Volume 31 Issue 6 Pages 756-762
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSEarly diagnosis and immediate treatment of the disease responsible for DIC are most important for successful therapy of DIC. Furthermore, it is also necessary to use anticoagulant agents in most cases of DIC.
The agents may be classified on the basis of their mode of anticoagulant action into three groups: ones with antithrombin effect, ones with anti-Xa effect or ones with both effect, and each agent is hoped to be chosen appropriately for development of DIC in near future. At present, such anticoagulant agents as standard heparin, antithrombin-III concentrate, gabexate mesilate, nafamostat mesilate, MD-805, low molecular weight heparin, heparan sulfate, activated protein C, are known as drugs for DIC, and each of them was effective for improvement from DIC in our experience. Antifibrinolytic agents, which have been considered to be contraindicated for therapy of DIC, may be good indication for selected cases of DIC with enhanced fibrinolysis such cases as acute promyelocytic leukemia. Antiplatelet agents may be available for some cases of chronic DIC.View full abstractDownload PDF (532K) -
Makoto MURATA, Masaaki HAYAKAWA, Kaoru GOTO, Kyouetu ISOBE, Tooru MATU ...1990 Volume 31 Issue 6 Pages 763-768
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSThree kinds of anticoagulant therapy for obstetrical DIC were studied. 1. Antithrombin-III (AT) or gabexate mesilate for acute DIC, mainly for abruptio placentae. 2. Heparin or heparin-AT combination therapy for toxemia pregnancy. 3. Low molecular weight heparin (LMWH) for fetus of intrauterine growth retardation (IUGR).
The results obtained were as follows,
1. a) Platelet count, and fibrinogen were significantly increased in AT therapy group compared with gabexate mesilate group.
b) In clinical manifestation, renal failure and hemorrhagic diathesis were improved especially in AT group.
2. In heparin-AT group, high systolic blood pressure was improved during administration of AT, the high level of thrombin antithrombin complex was also found in these period.
3. a) The improvement of the gain of estimated fetal body weight was found after administration of LMWH.
b) Redistribution of blood flow in one case of severe IUGR was observed during administration of LMWH.View full abstractDownload PDF (425K) -
Mutsuyoshi KAZAMA1990 Volume 31 Issue 6 Pages 769-775
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSTen controlled plasmas and 4 thromboplastin reagents were distributed to 75 laboratories for measurement of prothrombin time (PT). The results were converted to prothrombin activity (PA). prothrombin ratio (PR) and international normalized ratio (INR). Among the 4 different ways of expression, the discrepancy between the results obtained by the different reagents was the smallest when the results were expressed by INR.
However, the discrepancy of the results was still remained because of the different assay methods used routinely at these laboratories, even with the use of ISI/NR system (Instrumentation effects). In this study, reference curve of prothrombin time: INR was created using INR reference plasmas, which were selected from commercial control plasma using a standard thromboplastin reagent. INR's of 108 patients' plasmas were calculated by ISI/INR system on one hand, and by the use of the reference curve of the prothrombin: INR on the other hand. It was found that the discrepancy of the results obtained by different assay methods was significantly improved by the latter method, compared to those obtained by the conventional ISI/INR system.View full abstractDownload PDF (583K) -
Ikuro MARUYAMA1990 Volume 31 Issue 6 Pages 776-781
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSAnticoagulant as well as anti-platelet drugs are important medicines for the prophylaxis in various kinds of thrombotic diseases. However, the conventional anticoagulant drugs, heparin and coumarin congeners, have some disadvantages and limitations in clinical usage.
Recently new anticoagulants, both systhetic and recombinant, have been developing. They include synthetic thrombin inhibitor, recombinant hirudin, protein C and thrombomodulin. Here we reviewed synthetic thrombin inhibitor, Argipidine (MD805) in clinical trial and investigated its effect on thrombin catalyzed protein C activation on endothelial cells. Argipidine inhibited the protein C activating activity of thrombin on endothelium in a dose response manner. Next we examined the effect of Argipidine on thrombin-induced endothelin release from cultured endothelial cells. The augmentation of endothelin release from endothelial cells by thrombin was also inhibited by Argipidin. The effect was considered one of the advantage of this drug in the treatment of thrombosis.
Recombinant thrombomodulin had potent antithrombotic effect on thrombin-induced acute thromboembolism in mice, suggesting that this may be expectant anticoagulant for DIC or thromboses in human.View full abstractDownload PDF (472K) -
Tadao AKIZAWA1990 Volume 31 Issue 6 Pages 782-786
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSClinical characteristics of protease inhibitor, nafamostat mesilate (FUT-175: FUT), as an anticoagulant for extracorporeal circulation (ECC) were evaluated in hemodialysis and membrane plasmapheresis. FUT inhibited broad enzymatic systems including coagulation, platelet, complement, fibrinolysis and kinin cascades, which were commonly activated in the course of ECC. FUT showed regional anticoagulability, in which anticoagulating effects were firmly limited in extracorporeal circuit, and hemostatic time after ECC was significantly shortened by the use of FUT. FUT had no effects on lipid and bone metabolisms and FUT could not be adsorbed to anion exchange resin. These results indicate that FUT possesses superior characteristics as an anticoagulant for ECC to heparin, and FUT is very useful especially for ECC of patients with high bleeding risk.View full abstractDownload PDF (418K) -
[in Japanese], [in Japanese], [in Japanese], [in Japanese], [in Japane ...1990 Volume 31 Issue 6 Pages 787-792
Published: 1990
Released on J-STAGE: March 12, 2009
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Clinical and Cytogenetic Features in Childhood Acute Lymphoblastic Leukemia with 1; 19 TranslocationTakaaki SIKANO, Yorikazu ISHIKAWA, Mutsuko KONNO, Yukiko HATAYAMA, His ...1990 Volume 31 Issue 6 Pages 793-798
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe studied the clinical and cytogenetic features of 14 acute lymphoblastic leukemia (ALL) patients with 1; 19 translocation.
Ten patients had poor prognostic factors such as age over 10 years, hyperleukocytosis over 5×104/μl or high serum lactic dehydrogenase levels over 5,000 IU/l. Two patients had relapsed within 12 months after the onset, but their 5-year survival rate was 84.6%. Cytogenetically, 6 of 14 patients had multiple subclones. Two had the clones with hyperdiploidy >50 chromosomes, which was known to be one of the favorable prognostic factors in childhood ALL.
These findings show ALL children with 1; 19 translocation have a more favorable outcome in spite of some high-risk features than hitherto been thought.View full abstractDownload PDF (475K) -
Waichiro MIZUSHIMA, Mitsuoki EGUCHI, Hitoshi SAKAKIBARA, Kenichi SUGIT ...1990 Volume 31 Issue 6 Pages 799-806
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSBlasts from 5 cases of AML with pseudo-Chediak-Higashi granules were examined ultrastructurally and ultracytochemically using peroxidase, acid phosphatase, high iron diamine (HID) and periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) stainings. Pseudo-Chediak-Higashi granules, which apeared as vacuole-like inclusions by light microscopy, generally contained electron-lucent materials. All pseudo-Chediak-Higashi granules were, positive for peroxidase but some were negative for acid phosphatase. Pseudo-Chediak-Higashi granules were HID positive, indicating that they contained sulfated glycoconjugates. Glycogen-like particles were observed in the pseudo-Chediak-Higashi granules with the PA-TCH-SP method, as occasionally observed in granules in drug resistant ALL blasts. In conclusion, the contents of pseudo-Chediak-Higashi granules, which seemes to be formed by fusion of small granules, differed from those of normal azurophillic granules.View full abstractDownload PDF (1284K) -
Hiroaki NONAKA, Kazutaka KURIYAMA, Takao YANAGISAKO, Tomoko SATOH, Yas ...1990 Volume 31 Issue 6 Pages 807-812
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe have treated 20 adult patients with acute lymphoblastic leukemia (ALL) and 2 patients with lymphoblastic lymphoma with a protocol modified from L-10M of the Memorial Sloan Kettering Cancer Center. Eighteen patients (81.8%) entered complete remission (CR). Eight of them eventually relapsed (only 1 patient had a meningeal relapse) and died. Median CR duration was 19 months (median overall follow-up of 35 months and 50% remission duration was not yet determined. Median overall survival was 19 months. Three patients died of sepsis during remission induction, but all of other deaths were due to resistant or relapsed leukemia. The four patients who completed 3.5 year's modified L-10M protocol were free from relapse for 6-11 months (mean 8.7). Although further follow-up is necessary, we suggest that modified L-10M protocol is effective for adult ALL and long-term survival may be available.View full abstractDownload PDF (444K)
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Toru KIDA, Hajime NAKAMURA, Tetsuo KUROKI, Yasuhiro MIZOGUCHI, Kenzo K ...1990 Volume 31 Issue 6 Pages 813-819
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA case of hereditary stomatocytosis with hemolytic anemia, increased autohemolysis, increased osmotic fragility, and shortened erythrocyte survival is reported. The erythrocytes were abnormally permeable to sodium and potassium; the sodium concentration of the erythrocytes was high, and the potassium level was low.
This case was different from the first reported Japanease case of hereditary stomatocytosis of the hydrocytosis type in the Na+ -K+ ATPase and Mg+ ATPase activity. In the first reported Japanese case, these activities were within normal limits, but in this case, they were increased. The findings indicate that membrane transport in hereditary stomatocytosis of the hydrocytosis type may vary from case to case.View full abstractDownload PDF (605K) -
Akihiko OHNISHI, Shunjiro KAGIYAMA, Yukio SHINTATE, Nobuhiro KIMURA, A ...1990 Volume 31 Issue 6 Pages 820-826
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA 58-year old man was admitted because of general malaise in April 1987. Physical examination revealed systemic lymphadenopathy and hepatosplenomegaly. The white blood cell count was 252, 900/μl with 82% of blasts. Bone marrow aspiration contained 93.8% lymphoblasts, which were positive for TdT and negative for peroxidase reaction. Immunologic marker studies showed OKT 11 positive and CALLA negative. Cytogenetic analysis revealed a clone with 46, XY, t(9;22)(q34;q11), del(5)(q15) in 12 of the 13 metaphases. Ph1 positive T-acute lymphoblastic leukemia was considered. After AdVP and following AdVEMP (induction) chemotherapy, complete remission was obtained in August 1987. Cytogenetic study at the remission stage showed complete disappearance of Ph1 positive clone. Treatment with BH-AC DMP protocol at the time of recurrence in November 1987, brought no improvement and he died of respiratory failure. Chromosome study at recurrence showed an additional complex abnormal karyotype (double Ph1, +2, 5q-, -10, -13, -17).
DNA analysis revealed rearrangements of bcr gene with deletion of 5' side and of TCRδ gene, without any rearrangements in other immunoglobulin genes. From cytogenetic, immunophenotypic and genetic analysis the patient was diagnosed as having acute lymphocytic leukemia (FAB L1) with Philadelphia chromosome and rearrangements of bcr gene with deletion of 5' side and of TCRδ gene.View full abstractDownload PDF (504K) -
Akihiro OHMOTO, Michifumi KOHNO, Ryuji MATSUYAMA, Mikiya SATOH1990 Volume 31 Issue 6 Pages 827-830
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSOn November 22, 1985, a 54-year-old male was admitted to the cardiovascular department of our hospital suffering from thrombophlebitis, with reddness, swelling and pain aroud the right ankle and left knee. And he was transfered to our department on Nov. 26., because of hyperleukocytosis.
Peripheral blood examination revealed hyperleukocytosis with 93.0% blastic cells and thrombocytopenia. The bone marrow aspirate was hypercellular and almost all cells were consisted with peroxydase negative blastic cells. The blastic cells were Leu M1 positive and Leu M2, M3 and lymphocytic markers were negative. The patient was diagnosed to have acute lymphocytic leukemia with Leu M1 positive blast cells.
BH-AC/DMP thrapy was began on the 1st hospital day but he died of cerebral haemorrhage on the 4th hospital day. An autopsy revealed systemic infiltration of leukemic cells including thrombophlebitis of the legs.
Chromosome analysis of the bone marrow cells showed t(4;11)(q21;q23).View full abstractDownload PDF (412K) -
Masahiro IEKO, Norihiro SATOH, Shoki SAKURAMA, Yuki THOMA, Hiroshi HAN ...1990 Volume 31 Issue 6 Pages 831-836
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA 32 year-old male patient was admitted to our hospital because of abdominal tumor. The examination on admission showed massive splenomegaly and esophageal varices although peripheral blood cell counts were within normal limits. Exploratory laparotomy was performed with the diagnosis of portal hypertension and revealed the multiple thrombus formations in the splenic vein and the extramedullary hematopoietic findings in the spleen by the microscopic examination. In vitro colony forming assay showed the formation of spontaneous erythroid colonies in cultures of progenitor cells (from peripheral blood mononuclear cells) in erythropoietin-poor medium.
Increasing thrombocytosis was observed immediately after splenectomy, and hemorragic diathesis of nasal bleeding and gastrointestinal bleeding were also detected. The anlysis of plasma von Willebrand factor (vWF) revealed the decrease of ristocetin cofactor activity and the lack of large multimeric components of vWF. These abnormal findings observed after splenectomy led to recovery through the administration of busulfan with the improvement of thrombocytosis.
Accordingly, the course of the disease clearly indicated it to be the essential thrombocythemia represented as portal vein thrombosis and in latent form with normal cell counts in peripheral blood at the time of diagnosis, and subsequently, to develop into a full-blown form associated with acquired von Willebrand syndrome following splenectomy.View full abstractDownload PDF (587K) -
—Useful Application of KLI and G-CSF—Masaya NAGASU, Masaru NAKANO, Masuhira SAI, Shin AIZAWA, Keisuke TOYAM ...1990 Volume 31 Issue 6 Pages 837-841
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSThere has been increasing interest in autologus peripheral stem cell transplantation (APSCT) in the treatment of malignant desease because it is a convenient method and may have a lower risk of tumor cell contamination than autologus bone marrow transplantation (ABMT).
Recently, it is reported that the number of peripheral blood stem cells increase during recovery phase of hemopoiesis after chemotherapy, and further increase is reported on the case of treatment with G-CSF.
But influences of G-CSF to the residual malignant cells is still unknown. In this report, we have used clonal cell culture and kappa-lambda imaging (KLI) analysis to trace the level of CFU-GM, BFU-E, and malignant B-cell population (mBp) in peripheral blood before and after treatment with rhG-CSF.
The peak level of CFU-GM in peripheral blood was a 2-fold increased when rhG-CSF was administered, and this peak appeared 5-7 days earlier than that in the case without rhG-CSF.
MBp was detected in bone marrow, however, no mBp was detected before and after treatment with rhG-CSF in peripheral blood.
These findings suggest that APSCT is acceptable for this patient in case of minimal bone marrow involvement. And KLI analysis might be an effective method for detecting residual malignant cells in bone marrow and peripheral blood.View full abstractDownload PDF (369K) -
Toshio KAWATANI, Naoto MAEDA, Akihide HOSODA, Hiroatsu AGOU, Kazuhito ...1990 Volume 31 Issue 6 Pages 842-846
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA 76-year-old female was admitted to our hospital because of anemia.
Complete blood count was as follws: RBC 2.37×106/μl, Hb 7.7 g/dl, WBC 2,600/μl, Plt 105×103/μl. A bone marrow aspirate revealed 40.8% plasmacytoid cells showing the characteristics of plasma cells by electron microscopy. Total serum protein was 5.4 g/dl. Monoclonal protein was not observed by electrophoresis. On immunoelectrophoresis, M-bow was not observed in the serum or in 50-fold concentrated urine. The plasma cells were negative for cytoplasmic IgG, M, A, E, D, kappa or lamda by immunoperoxidase studies.
Although radiologic studies of the bones did not reveal destructive or punched out lesions, we diagnosed this case as a nonproducing myeloma and the patient responded to MP therapy. This case was considered interesting as regards the pathological entity of myeloma.View full abstractDownload PDF (402K) -
Mitsuhiro ITO, Toshitaro NAKAGAWA, Shion IMOTO, Takashi ISOBE, Yoshino ...1990 Volume 31 Issue 6 Pages 847-852
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA 46-year-old man was admitted because of lumbago and numbness of the left leg. Pelvic X-ray showed a large defect in the left sacrum and CT revealed multiple punched-out lesions. Serum IgA was 1,740 mg/dl with a monoclonal component of IgAκ by serum electroimmunofixation. Bence Jones protein of κ type was detected in urine. Diagnosis of myeloma was made on the basis of histology of the biopsied sacral tumor. Repeated melphalan/prednisolone intermittent therapy (MP) was done with concomitant administration of natural interferon-α (IFN-α) 3×106 U intramuscularly for 67 days. Performance status including gait markedly improved. Normal bone marrow morphology and disappearance of M-protein by electroimmunofixation were achieved after 13 cycles of MP, when pelvic X-ray revealed prominent recalcification. No further treatment was instituted for subsequent 6 months, without any demonstrable M-protein.
Complete remission of myeloma is rare with conventional therapies and thus new therapeutic modalities have been waited for. IFN-α may promise better responses if appropriately combined with other chemotherapies.View full abstractDownload PDF (790K) -
Hiroko SHIOZAKI, Chikara IWAHASHI, Tadaomi SUZUKI, Kazuo MIYOSHI, Riic ...1990 Volume 31 Issue 6 Pages 853-857
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe report a case of AML (M 4) with eosinophilia who developed meningeal relapse and transverse myelopathy.
A 37-year-old woman was admitted to our hospital because of lymphadenopathy and ecchymosis. One week prior to admission, she noticed swelling of the cervical lymph nodes and bleeding tendency. On admission, low-grade fever, gingival swelling, generalized lymphadenopathy, and ecchymosis on the lower legs were found. A white blood cell count was 93,900/μl with 82% blast cells, and a platelet count was 24,000/μl. A bone marrow was composed of 45.3% myeloblasts, 27% monocytes and 7.1% eosinophils. Chromosome analysis revealed inv (16). The diagnosis of M4Eo was made. About one year after she gained complete remission, she was readmitted because of disturbance of urination. There was a sign of transverse myelopathy at the seventh vertebral level, and blast cells were detected in the cerebrospinal fluid. Despite of radiation and chemotherapy, paresis of lower extremities and sensory disturbance were persistent, and the patient died on 52th hospital day.View full abstractDownload PDF (374K)
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Successful ATG and Bolus Methylprednisolone Therapy towards Severe Aplastic Anemia with SplenomegalyYoshihiro MATSUKAWA, Yoshihiro HATTA, Mitsumasa SHIDA, Masumi BABA, Sh ...1990 Volume 31 Issue 6 Pages 858-859
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA 22-year-old female patient with severe aplastic anemia was treated by ATG·bolus methylprednisolone therapy. After the therapy, anemia and thrombocytopenia began to recover within a month. The frequency of blood transfusion decreased thereafter. Since anemia due to splenomegaly was certified, bolus methylprednisolone therapy was performed in good response. Suppressor T-cells towards hemopoiesis were confirmed in this case, and hemopoietic potential was augmented by ATG·bolus methylprednisolone therapy. In spite of discontinuation of any therapy in 1986, she is being in good health more than five years.View full abstractDownload PDF (169K) -
Koichi SHIMMYOZU, Naoko KADOKURA, Mitsutoshi TARA, Ikuro MARUYAMA, Mit ...1990 Volume 31 Issue 6 Pages 860-861
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSIn order to elucidate the response of protein S (PS) and C4b-binding protein (C4bp) to danazol, we investigated the serial changes in plasma levels of PS and C4bp during danazol administration in 4 patients with endometriosis. Both total and free PS levels were significantly increased at the time of 2 to 3 weeks after danazol administration as compared to the pretreatment values, while C4bp levels were not changed at all.
These results imply that danazol administration to PS deficiency would produce a significant increase in the basal level of free PS, suggesting a potential adjunct to anticoagulant treatment in PS deficiency.View full abstractDownload PDF (154K)
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1990 Volume 31 Issue 6 Pages 862-872
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (1011K)
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1990 Volume 31 Issue 6 Pages 873-884
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (1076K)
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1990 Volume 31 Issue 6 Pages 885-889
Published: 1990
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSDownload PDF (499K)
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