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Volume 32, Issue 6
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Akio KANZAKI, Hideho WADA, Yoshihito YAWATA1991 Volume 32 Issue 6 Pages 573-579
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSCytoskeletal abnormalities in red cells were studied in 250 patients at our laboratory, especially on hereditary elliptocytosis (HE), hereditary spherocytosis (HS), and band 4.2 anomalies. First of all, on HE, we have found two patients of a dominantly-inherited trait of a new β-spectrin variant with 216 kDa peptide. The contents of abnormal β'-spectrin to the total spectrins were 7.6% in propositus, and 10.5% in her mother. As a functional abnormality, abnormal αβ'-spectrin dimer could not be converted to tetramer in both patients. Thus, our patients may differ from HE Nice. Secondarily, the causal relationship between red cell ankyrin and spectrin was studied on a typical HS case with abnormal chromosome, del (8p11.2-8p21.1). In this case, the deleted genetic locus was involved with loci of genes for SPH1 and ankyrin. The contents of ankyrin and other red cell membrane proteins, however, appeared to be normal on SDS-PAGE. In addition, as a unique disorder in Japan, unrelated five cases of membrane protein 4.2 deficiency were found at our laboratory. In these cases, the characteristic features were; 1) clinically uncompensated hemolysis, 2) ovalostomatocytosis, 3) markedly decreased deformability of the intact red cells by ektacytometry, if heat-treated, 4) two peptides of membrane protein 4.2 were detected in a trace amount by Western blot. Five phenotypes were categorized by Western blot, as based on the type of membrane protein 4.2 present; 1) 72 kDa peptide alone, 2) 72 kDa+74 kDa in a trace amount, 3) 72 kDa+74 kDa both in a trace amount, 4) 72 kDa+68 kDa in a trace amount, and 5) complete deficiency.View full abstractDownload PDF (731K) -
Yutaka KOHGO, Hitoshi KONDO, Michiaki HIRAYAMA, Nobuyasu TSUSHIMA, Yos ...1991 Volume 32 Issue 6 Pages 580-586
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSOne of the most important factors for the proliferation and hemoglobin synthesis of erythroid cells is iron atom. This atom is tightly bound to serum transferrin (Tf) and is taken up by etythroblasts and reticulocytes through transferrin receptor (TfR). Both Tf and TfR are reutilizable and have roles for the efficient intracellular accumulation of iron. In addition to the reutilization (recycling), the expression of TfR is also regulated by cytoplasmic iron concentration; the increase of iron downregulate the synthesis of TfR at the translational level and vice versa. This mechanism was recently explained by the binding between “iron responsive element (IRE)” in the 5' end of TfR mRNA and IRE binding protein by a transacting manner. Johnstone et al, and we found that TfR was externalized from sheep reticulocyte and humanerythroleukemia cell, K562, respectively. Furtheremore, we confirmed that this shed TfR was detected in blood and concluded that the quantitation of TfR in serum is a useful index for evaluating the erythropoiesis. The serum TfR was increased in iron deficiency anemia, hemolytic anemia and polycythemia and was decreased in aplastic anemia. In renal anemia, it was increased after the administration of eythropoietin (Epo). By the in vitro liquid culture of peripheral blood stem cells using interleukin 3 and Epo, it was found that soluble TfR was derived from the erythroblasts during the maturation process.View full abstractDownload PDF (589K) -
Yasuyuki FUKUMAKI1991 Volume 32 Issue 6 Pages 587-591
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSMolecular analysis of gene structure using PCR related techniques has been described. These techniques were applyed to analysis of the β-globin gene from Japanese individuals with β-thalassemia. We found one promoter mutation, two splicing mutations, two frameshift mutations, one nonsense mutation. We also detected three different mutations within the third exon. They produced highly unstable globin variants, leading a dominantly inherited β-thalassemia phenotype. Six of nine different mutations seem to originate in Japanese. Characteristics of molecular defects of Japanese β-thalassemia was discussed in terms of malaria hypothesis.View full abstractDownload PDF (455K) -
Yukio OHHARA, Yoshitoshi MAE, Yoshio UEHARA1991 Volume 32 Issue 6 Pages 592-598
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSQuantitative and qualitative evaluations of erythrocyte ferritin in 161 patients with RA and RAEB in MDS, AML, CML, PV, PA, HS, IDA, chronic liver disease and alcoholic liver disease were carried out. Mean erythrocyte ferritin levels of patients with RA, AML, PA, HS and alcoholic liver disease were increased compared with normal subjects. On isoelectric focusing analyses (IEF), erythrocyte ferritin in normal subjects were detected between pI 5.1 and 5.7. In the cases of RA, pI ranges of erythrocyte ferritin may be divided into three groups, acidic, neutral, basic shift on IEF respectively. In these groups, the more acidic the ferritin shift, the higher the proportion of morphological abnormalities of the erythroid precursors in the bone marrow was observed. In patients with AML (M2, M3, M4), little difference was found among these three subtypes, and all of the cases showed similar pattern with normal subjects on IEF. The ferritin from IDA showed low levels and slight basic shift compared with normal subjects on IEF, and these features were also found in patients with CML (chronic phase) and PV. After iron supplementation, marked increase of acidic ferritin was detected on IEF indicating an intermediate store for iron destined for haem synthesis. It was clear that the stainable iron in liver parenchymal cells were found at erythrocyte ferritin concentration 20 ag/cell or over in patients with chronic liver disease. Measurement of erythrocyte ferritin concentration is a helpful method for evaluating iron deposition in hepatocyte non-invasively. From these rusults it is considered that quantitative and qualitative analyses of erythrocyte ferritin are very useful for evaluating erythropoiesis as well as iron metabolism.View full abstractDownload PDF (457K) -
Reiji KANNAGI, Koichi ZENITA, Tokugoro TSUNEMATSU1991 Volume 32 Issue 6 Pages 599-605
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSAnti-erythrocyte antibodies which appear in the sera of patients with auto-immune hemolytic anemia frequently recognize carbohydrate auto-antigens. Most of cold agglutinins are known to recognize the Ii-antigens, and Donath-Landsteiner antibodies which appear in patients with paroxismal cold hemoglobinuria are almost exclusively directed to the P-antigen. These carbohydrate auto-antigens are strongly expressed in various tissues and organs other than erythrocytes, and behave as differentiationor developmental-antigens, in both humans and mice. The study of nucleotide sequences of human and murine anti-Ii antibodies shows that these antibodies share a highly homologous antigen-binding site in their VH regions. These results indicate that the carbohydrate auto-antigens in autoimmune hemolytic anemia are evolutionally conserved developmental antigens, and suggest that the immunoglobulin genes which encode variable regions of the auto-antibodies directed to these antigens are also conserved evolutionally.View full abstractDownload PDF (812K) -
Hideki NAKAKUMA, Tatsuya KAWAGUCHI1991 Volume 32 Issue 6 Pages 606-611
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSParoxysmal nocturnal hemoglobinuria (PNH) is currently accepted to be a stem-cell disorder of a clonal nature with increased susceptibity to autologus complement attack. Consequent hemolytic feature has been partly explained by lack of complement regulatory membrane proteins such as decay-accelerating factor (DAF) or C8-binding protein that anchored to membrane via glycosyl-phosphatidyl inositol (GPI) lipids. Recent reports suggest esssential PNH lesion is the synthetic defect of sugar moiety of the PI-anchor. In PNH, the abnormal expression of C3b/C4b receptor (CRI) glycoproteins, or glycophorin-α have been also pointed out. These altered expression of glycoproteins and glyceroglycolipids, especially in the carbohydrate structures, prompted us to analyze biochemically the membrane glycosphingolipids as one of major glycoconjugates in PNH. As results, PNH erythrocytes showed altered metabolism of gangliosides in comparison to control erythrocytes from healthy donors. IV6 NeuAc-nLc4 Cer and highly polar gangliosides variably disappeared in PNH erythrocytes, partly due to impaired sialylation of glycolipids. These results suggest metabolic disorder of carbohydrates of membrane glycoconjugates as a new aspect of PNH.View full abstractDownload PDF (499K) -
Haruhiko NINOMIYA, Toshitaka KOBAYASHI, Tsukasa ABE1991 Volume 32 Issue 6 Pages 612-617
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSThe significance of the deficiency of the major complement-regulatory membrane proteins, decay-accelerating factor (DAF) and CD59, to the lysis of paroxysmal nocturnal hemoglobinuria (PNH) red blood cells was investigated. DAF and CD59 were demonstrated to be deficient simultaneously on affected PNH red blood cells (PNH-III) by two-color FACS analysis. At least in some patients with PNH, PNH-I was also revealed to be deficient partially in DAF. Purified DAF and CD59 ameliorated the complement sensitivity of PNH red blood cells, partially and completely, respectively. Functional blocking of these molecules on normal human red cells by monoclonal antibodies to DAF and CD59 rendered A or AB type blood cells complement-sensitive but not O or B blood type blood cells. The differences of complement-sensitivity among blood types were revealed to reside on the step of binding of C9 to C5b-8, i.e. C9 can bind to C5b-8 more on A type blood cells than on O type blood cells. We conclude that the deficiency of DAF and CD59 play a major role for the complement sensitivity of PNH red blood cells and that other factors reported to be deficient in PNH do less than these two proteins.View full abstractDownload PDF (442K)
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Keiki KAWAKAMI, Takeshi IKEDA, Kenkichi KITA, Shigeru SHIRAKAWA1991 Volume 32 Issue 6 Pages 618-622
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSTo identify the biological characteristics of so called stem cell leukemia (SCL), of which leukemic blast cells should be derived from pulripotent stem cells, immunophenotypical and genotypical analysis and response to several hematopoietic cytokines were studied in 272 cases with acute de novo leukemia. In 132 cases with acute myelogenous leukemia (AML), some cases of CD19+ and/or CD7+ AML were considered as SCL. In cases with myeloperoxidase negative acute lymphoblastic leukemia (ALL), cases of CD7+CD1-CD3-CD4-CD8-My-Ag (myeloid antigens)+ALL, considered as those of T-precursor ALLs, and cases of HLA-DR+CD19+CD20-My-Ag+ALL, considered as those of B-precursor ALLs, were though to be SCL. We did not think the cases of ALL with dual genotype to be SCL, since dual genotype could not be considered as sings of ability to differentiate to multilineage but as products of the process of active V-DJ rearrangements of Ig heavy chain gene.View full abstractDownload PDF (401K) -
Yasuyuki KUNIEDA, Mihiro OKABE1991 Volume 32 Issue 6 Pages 623-628
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe have carried out the molecular and cell-biological analysis on Ph1-positive leukemias in this study. Five out of nine Ph1-positive ALL cases showed molecular rearrangement within the classical bcr sequence (or M-bcr), similar as those in 47 CML cases. We examined 4 cases of Ph1-positive ALL presenting no rearrangement of M-bcr and found that, in 2 of 4 cases, one showed the breakpoint in a 5 kb segment of the bcr gene first intron (bcr-2) and the other in bcr-1, 16 kb upstream of bcr-2. Ph1-positive ALL frequently showed biphenotypical or biclonal phenotypes of myeloid and lymphoid lineages. Furthermore, we demonstrated the ability of two Ph1-positive ALL cell lines to differenciate into monocytic leneage in vitro, thus suggesting the posibility that these Ph1-positive ALL cells might reside on the stage of multipotent stem cell along the heamatopoietic cell differenciation. Two out of 31 CML cases showed the mutations of the ras genes by the polymerase chain reaction; one case in the crisis phase and the other in the chronic phase. However, no mutations of the fms genes was detected. Two cases in the crisis phase of 24 CML patients (11 cases in the chronic phase and 13 cases in the crisis phase) contained rearrangements of the p53 gene by Southern analysis. Furthmore, the transcriptional alteration was found in 2 CML-BC and 2 CML-BC derived cell lines' samples, suggesting a important role of the p53 gene in the transformation of CML into the crisis phase.View full abstractDownload PDF (618K) -
Norio ASOU1991 Volume 32 Issue 6 Pages 629-635
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe studied gene rearrangement and expression of immunoglobulin heavy (IgH) chain, T cell receptor (TCR) β, γ and δ chains in neoplastic T cells from patients with leukemia and lymphoma. Rearrangements of TCR β and γ chain genes were observed in most of T cell neoplasms. TCR δ chain gene rearrangments or deletions were detected in all 77 T cell neoplasms; 6 of 9 CD3- T cell neoplasms showed rearrangement, whereas biallelic deletion of TCR δ chain gene was the most common pattern in CD3+ T cell neoplasm (65 of 68 patients). One patient with CD3- T cell leukemia had TCR δ chain gene rearrangement with a germline configuration of TCR β, γ and IgH chain genes. TCR γ and δ chain gene transcripts were detected in most of the CD3- T cell neoplasms, whereas mature TCR α and β chain mRNA were demonstrated in the majority of the CD3+ T cell neoplasms. In 6 patients with CD7+ CD3- CD4- CD8- MPO- leukemia, only 2 patients had rearrangements and weak expressions of IgH, TCR γ and δ chain genes. We also present two cases of double negative (CD3+ CD4- CD8-) leukemia; one is TCR γδ bearing LGL, the other is TCR αβ bearing ATL. These results suggest that most of T cell neoplasms preserve a pattern of genotypic and phenotypic expression reflecting their developmental pathways and defferentiation levels of TCR bearing normal T cells.View full abstractDownload PDF (523K) -
—A Study using the Polymerase Chain Reaction—Toshiyuki MIYASHITA, Minoru ASADA, Shuki MIZUTANI1991 Volume 32 Issue 6 Pages 636-643
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSA leukemia line KOPN30bi was established from a patient of acute lymphoblastic leukemia with Philadelphia chromosome. The clonal rearrangement of the immunoglobulin heavy chain gene was identical between KOPN30bi and the predominant clone in the fresh sample (S1) from which KOPN30bi was established, indicating that they are of the same clonal origin. The study of the T cell receptor (TCR) genes including TCRβ, γ, δ loci showed none of these loci was identical between KOPN30bi and S1. The result of the TCRδ region analysis which was rearranged on one of the alleles in KOPN30bi and was deleted on both alleles in S1, however, indicated KOPN30bi was not a derivative of S1. Polymerase chain reaction, using oligonucleotide probe corresponding to the N region sequence of Vγ-Jγ juncture of KOPN30bi, indicated that only one % of the blast cells in S1 corresponded to KOPN30bi. These studies indicated that the predominant clone in the fresh sample, although it occupied more than 99% of the blasts, did not represent the characteristics of the target cell for leukemogenesis, and furthermore that the leukemogenic molecular mechainsms such as P190 type BCR/ABL translocation are not enough to freeze the differentiation of the target cell.View full abstractDownload PDF (674K) -
Masao SETO, Hirotaka OSADA1991 Volume 32 Issue 6 Pages 644-649
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSIn t(14;18) lymphomas, bcl-2 gene is activated by the juxtaposition of immunoglobulin (Ig) gene. The fused bcl-2-Ig gene generates chimeric mRNAs which consist of bcl-2 at 5' side and Ig at 3' side. Chimeric mRNA does not disrupt the bcl-2 coding frame of 239 amino acid polypeptide. Activated bcl-2 gene introduced in normal B lymphoblastoid cells (LCL) demonstrated an increased cloning efficiency in soft agar but failed to confer tumorigenicity to LCLs as a single agent. bcl-2 gene rearrangement in Japaneses B cell lymphoma was studied and found that 10 out of 32 cases of follicular lymphoma (31%) and 5 out of 56 cases of diffuse lymphoma (9%) were rearranged, suggesting less frequency of B cell lymphoma, particularly follicular lymphoma in Japan is partly due to less bcl-2 involvement than American cases. Three cases out of 15 cases with bcl-2 rearrangement demonstrated a unique pattern of rearrangement. Two cases of the three were analysed and found that both cases were translocated at the later step than DH-JH joining of Ig rearrangement. Thus, bcl-2 translocation in Japanese B cell lymphomas might occur at the later stage of B cell development, when compared with that in American cases. Less involvement of bcl-2 in Japanese B cell lymphoma may be explained by low susceptibility to bcl-2 rearangement at the step of DH-JH recombination.View full abstractDownload PDF (427K) -
Masaaki ADACHI, Kohzoh IMAI, Akira YACHI, Yoshihide TSUJIMOTO1991 Volume 32 Issue 6 Pages 650-654
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSMost of human follicular lymphomas (∼90% in U.S.A. or ∼30% in Japan) possess the t(14;18) chromosome translocation that directly involves the IgH locus on chromosome 14 and the bcl-2 gene on chromosome 18. The t(14;18) chromosome translocation occurs nearly exclusively at two hot spots, a major breakpoint clustering region (mbr) within the 2nd exon noncoding region and the minor breakpoint clustering region (mcr) within the 3' flanking region of the bcl-2 gene. Here we show that the rearrangement of the bcl-2 gene occurs in a signicant fraction (∼10%) of B-CLL. All of the rearranged bcl-2 genes were juxtaposed with Igλ or Igκ genes, implying that the bcl-2 gene is preferentially linked to the IgL genes in CLL.View full abstractDownload PDF (380K) -
Hitoshi OHNO1991 Volume 32 Issue 6 Pages 655-659
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSThe 14;19 translocation [t(14;19)(q32;q13)] is a recurring chromosomal translocation observed in leukemic cells of chronic lymphocytic leukemia showing prolymphocytoid transformation. We have cloned the breakpoint junction of the translocation and identified a new gene, bcl-3, on the chromosome band 19q13 adjacent to the breakpoint. The translocation ocurred at the switch region of the α constant locus of the immunoglobulin heavy chain gene and the upper stream of the bcl-3 gene, resulting in a head-to-head recombination between the two genes on the 14q+ chromosome. The bcl-3 gene was highly expressed in the leukemic cells carrying the 14;19 translocation. The bcl-3 gene product contained repeat structures found in proteins involved in cell cycle control and cell lineage determination. These results suggest that the bcl-3 is a proto-oncogene that may contribute to the development of leukemias carrying the 14;19 translocation.View full abstractDownload PDF (361K) -
Eiji TATUSMI, Noriko YONEDA1991 Volume 32 Issue 6 Pages 660-668
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSThe results of DNA or RNA study in EBV-associated lymphoproliferative diseases were shown. For detecting EBV DNA, Southern blot analysis with Bam HIW probe (Internal Repeat) and non-repeat (least often deleted) probe are used. Probes close to (ex. LMP) or within terminal repeat can indicate clonality in terms of junctional structure. Several such examples were shown, in which benign polyclonal EBV (+) CD3+8+ lymphocytes, EBV (+) CD3+4-8- granulay lymphocytosis, EBV (+) t(14,22) B-cell lymphoma and EBV (−) follicular lymphoma with reactivation type serology were included. The detectability of EBV DNA was tested in consecutively sampled acute IM peripheral cells by Southern blot analysis with Bam HIW, PCR with Bam HIK (EBNA1) and its Southern re-estimation. The results indicated that EBV DNA was detectable rarely in the earliest samples, and that PCR can increase the sensitivity, as expected. The gene expression of IL-2Rα (−) IL-2Rβ (+) and perforin (−) by IM cells were assessed with Northern blot analysis. The abundunt γIFN gene expression by IM cells was revealed by reversed PCR.View full abstractDownload PDF (788K)
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Masanori UMEDA, Tatsuo SHIRAI, Toshihiro TSUKAHARA, Haruo KANEKO, Miki ...1991 Volume 32 Issue 6 Pages 669-674
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe compared the effect of combined natural interferon-α (HLBI)/VCAP chemotherapy with VCAP chemotherapy alone on multiple myeloma. Sixteen previously untreated patients with multiple myeloma were treated with a combination of IFN-α and VCAP chemotherapy; nine of them were treated with HLBI-VCAP (I) regimen (HLBI 3×106 units/day, daily, 56 days) only for induction chemotherapy, and seven with continuous HLBI-VCAP (II) regimen (HLBI 3×106 units/day, twice a week) both for induction and maintenance chemotherapy. Thirty-one control patients were treated with a VCAP regimen only. HLBI-VCAP (II) regimen exhibited an 85.7% (6/7) response rate, while VCAP and HLBI-VCAP (I) regimen showed 74.2% (23/31) and 77.8% (7/9) response rates, respectively. The median duration of survival was 43 months in the control group, >44 months in HLBI-VCAP (I) group and >45 months in HLBI-VCAP (II) group. No significant difference in survival duration has yet been observed between the VCAP group and HLBI-VCAP groups. We conclude that continuous, long-term combination therapy with HLBI and VCAP regimen for induction and maintenance therapy may be most effective, and found that intensive HLBI-VCAP regimen, for only remission induction therpy, was not more effective than VCAP regimen alone.View full abstractDownload PDF (435K) -
Akihiko CHUBACHI, Akira B MIURA, Shigeki NISHIMURA, Tetsuo AKIHAMA, Ak ...1991 Volume 32 Issue 6 Pages 675-680
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSTen patients with non-Hodgkin's lymphoma originated in the nasal cavity (four patients) and in the paranasal sinuses (six patients) were treated mainly with irradiation and combination chemotherapy including adriamycin. According to the TNM AJC staging system, four patients were in stage T1-T2, and six patients were in stage T3-T4. Nine patients, other than one with stage IV (Ann Arbor) disease, achieved complete remission. Death due to lymphoma occurred in four patients, 4 to 39 months following diagnosis. Three of these patients developed systemic extranodal dissemination, and died in a short time after relapse. Death due to second malignancies occurred in two patients. One died of acute myelogenous leukemia, and the other died of colon cancer, 26 and 53 months after diagnosis, respectively. Four patients were alive and disease-free, from 23 to 68 months following diagnosis (median 40 months). Out of four patients who died of disease, three were in stage T3-T4, and one was in stage T1. Two patients with stage T1 originated in the nasal cavity were both alive and disease-free. Except for lymphomas with stage T1 originated in the nasal cavity, more intensive chemotherapy should be instituted in an attempt to achieve better disease-free survival.View full abstractDownload PDF (498K)
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Shion IMOTO, Mitsuhiro ITO, Toshitaro NAKAGAWA1991 Volume 32 Issue 6 Pages 681-685
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe experienced a remarkable effect of recombinant interferon α2a (α-IFN) in a case of angioimmunoblastic lymphadenopathy with dysproteinemia (AILD) which was refractory to steroids and combination chemotherapies. A 62 year-old woman was admitted because of high grade fever and extreme swelling of cervical lymph nodes. Poly-clonal hypergammaglobulinemia and plasma cell-like atypical lymphocytosis in the peripheral blood were demonstrated. Cervical lymph node biopsy disclosed histology of AILD. She initially responded well to prednisolone. Three months later. AILD relapsed in spite of prednisolone treatment. She received combination chemotherapies and responded well again. Seven months later, she became refractory to these combination chemotherapies. Consequently, we tried α-IFN (3 million units/day given intramuscularly). She became afebrile on the next day, and lymph nodes swelling gradually disappeared. She has been free from the disease for more than three months.View full abstractDownload PDF (682K) -
Toshiki NATAZUKA, Ryoichi OGAWA, Yasukazu FUJII, Mizune SUZUKI, Toshim ...1991 Volume 32 Issue 6 Pages 686-689
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe report a case of ectopic salivary amylase-producing IgA-λ-type multiple myeloma. A 70-year-old man was admitted because of anemia and renal failure. After chemotherapy for eight months, the serum amylase markedly increased. Amylase activity in the supernatant of cultured myeloma cells, which were obteined from the bone marrow, also increased. The myeloma cells expressed MDR-1/P-glycoprotein detected by flow cytometry with MRK-16 (monoclonal antibody of MDR-1/P-glycoprotein). The case implies the association of drug resistance and the ectopic amylase production in a case of multiple myeloma.View full abstractDownload PDF (350K)
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Hideo NAKAMURA, Seiji TOKUNAGA, Naoki SADAMORI, Masao TOMONAGA, Michit ...1991 Volume 32 Issue 6 Pages 690-692
Published: 1991
Released on J-STAGE: March 12, 2009
JOURNAL RESTRICTED ACCESSWe performed cytogenetic studies and breakpoint cluster region (bcr) rearrangement analysis in two cases of juvenile chronic myeloid leukemia (JCML) which is special type of chronic myeloid leukemia (CML). Case 1 (8-month-old male) and case 2 (3-month-old female) showed clinical and hematologic manifestations similar to CML. Each of case 1 and 2 had normal karyotype and no bcr rearrangement. These findings suggest that JCML is a defferent heterogeneous disorder from that of adult CML.View full abstractDownload PDF (201K)
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