Effects of grafting to medium size tomato ‘Hanakomachi’ onto rootstock varieties ‘Ganbarune forte’, ‘Spike23’ and ‘Fortamino’ were evaluated on the Sprayponic in order to achieve high yield..‘Hanakomachi’ became vigorous onto three varieties to get higher yield on the ‘Spike23’ and ‘Fortamino’ significantly. Root average diameters of each rootstock varieties were ranked as ‘Fortamino’ > ‘Spike23’ > ‘Ganbarune forte’ > ‘ Hanakomachi’ (scions), grafting made their root thicker. Specific root length of ‘Fortamino’ has significantly reduced and stem fresh weight of ‘Spike23’ has significantly increased. Especially ‘Hanakomachi’ grafted to ‘Fortamino’ was considered keeping vigorous at the end of the cultivation period and might contribute to higher yield, because of the higher sink activity of root indicated by specific root length and higher physiological activity indicated by brighter root color.