ADVANCES IN OBSTETRICS AND GYNECOLOGY

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This study attempts to determine the transition of ultraviolet absorbing constituents in amniotic fluid by using high pressure liquid chromatography in which strong basic anion exchanging resin, CDR-10 is used and it also hopes to investigate physiological significance and to develop a new procedure to determine fetal maturity.
Ultraviolet absorbing constituents in term amniotic fluid were separated to 20 peaks at least. It was identified that main peaks were creatinine and nucleotide related metabolites, namely hypoxanthine, xanthine and uric acid. They increased along with fetal development and a quantitative change of each constituent was recognized. The total peak area increased remarkably after 35gw and 2.6cm² of peak area was a suitable marker for discriminating 35 gw. The relations between that total peak area and fetal body weight showed a equation line of Y=0.0092+0.74 with r=0.60. It was noted that the origin of these substances was fetal urine. Such increase must reflect the rise of metabolism accompanied by fetal development and maturation of fetal renal function.
In order to study the aspect of nucleic acid metabolism of fetal liver, catabolic enzyme of xanthine oxidase and synthetic enzyme of adenylosuccinate sythetase were determined by using rat's liver. As a result, it was found that during fetal age, synthetic phase was dominant but activity of catabolic phase was increased gradually.
On the basis of those results, a clinical significance of measuring OD₂₅₄ in amniotic fluid was discussed.

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