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Volume 41 (1989)
- Issue 6 Pages 769-
- Issue 5 Pages 681-
- Issue 4 Pages 561-
- Issue 3 Pages 293-
- Issue 2 Pages 173-
- Issue 1 Pages 1-
Volume 41, Issue 2
Displaying 1-10 of 10 articles from this issue
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Kaoru NABUCHI, Ikuko MORIYAMA, Hideaki IIOKA, Yuko NINOMIYA, Masayoshi ...1989 Volume 41 Issue 2 Pages 173-178
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSTo prevent metabolic bone diseases in premature neonates, a sufficient supply not only of vitamin D but also of calcium and phosphorus is of major importance. In order to determine the required level of phosphorus, the phosphorus concentration in umbilical cord blood and in the blood of a week-old infant, the phosphorus concentration in the urine directly after birth, and the %TRP were measured. The capacity to reabsorb inorganic phosphorus was also studied using brush border membrane vesicle from tubular epithelium in the kidneys.
1 The concentration of inorganic phosphorus in umbilical cord blood was twice as high as the blood concentration in week-old infants.
2 The concentration of inorganic phosphorus in the urine 24 hours after birth was negligible in neonates born after 32 gestational weeks or more.
3 The capacity to reabsorb inorganic phosphorus was studied using brush border membrane from tubular epithelium in the kidneys.
1) The reabsorption mechanism displayed sodium dependent active transport.
2) Reabsorption of inorganic phosphorus was found from about the 24th week of pregnancy, and at 32 weeks it reached the same level as at full term delivery.
As a result of these findings, we concluded that it is necessary to provide supplementary phosphorus to small premature neonates, particularly those that are breast-fed, even if the blood concentration of phosphorus is normal.
For premature neonates whose adjusted number of gestational weeks at the time of birth is 32 weeks or less, other indications must be taken into account and supplementary phosphorus be provided even if the % TRP is low.View full abstractDownload PDF (845K) -
Yoshiaki OKAMOTO, Masatsugu UEDA, Takashi YAMADA, Osamu MISAKI, Takemi ...1989 Volume 41 Issue 2 Pages 179-184
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSWe studied the Thomsen-Friedenreich (T) antigen in uterine cervical cancer tissues and cell lines, and their changes by drugs. We stained tissues and culture cells by way of Abidin-Biotin-peroxidase Complex (ABC) method using a monoclonal antibody to the T antigen. The antibody was obtained by immunizing mouse with asialo-glycophorine. The results were as follows. 16 of 24 (66.7%) tissues were stained positive, and the cell line OMC-1 and OMC-4 were positive in cytoplasms. As the T antigen appeared frequently in uterine cervical cancer tissues and culture cells, we can use the T antigen as the tumor marker for the uterine cervical cancer. The T antigen was not changed so much by 5FU and EGF in OMC-1 and OMC-4 from our study using the Enzyme-Immuno-Assay (EIA). So the T antigen is concerned with modulation of the uterine cervical cancer, but not so much with damages and growth of it.View full abstractDownload PDF (2688K) -
Junichi SASAKI, Takara YAMAMOTO, Mamoru URABE, Hiroji OKADA1989 Volume 41 Issue 2 Pages 185-188
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSThe metabolic fate of the antiprogestogen agent RU38486 was examined in the livers of rabbits and rats. After portal injection, only a small portion of RU38486 was taken in the liver, and metabolized to RU42633, RU42848 and RU42698. There was no difference in the metalolism of RU38486 between the rabbit and rat liver. RU42633, which possesses high bioactivity of antiprogesterone, was the major metabolite of RU38486.View full abstractDownload PDF (509K) -
Jun TAKEYAMA1989 Volume 41 Issue 2 Pages 189-201
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSFlow cytometry was used to investigate the effects of radiation upon L-1210 cell kinetics. Nuclear DNA histograms revealed G2, M phase accumulations and the development of regions of hypertetraploidy. The amounts of BrdU and DNA were measured simultaneously. One effect of irradiation was a decrease in the S phase fraction, in other words, the DNA synthetic rate. As dosages were increased, accumulations were observed to a point which seemed to indicate late S and G2, M phases ; and finally DNA synthesis terminated. Low dosage DNA histogram changes were reversible but high dosage changes were not. Using a cell sorter confirmed that while mutinuclear and giant cells accumulate in the hypertertraploid region, ghost cells accumulate in the hypodiploid region.
Flow cytometric DNA analyses of uterine cervical cancers were performed examining histological pareffin-embedded operative tissues. DNA ploidy was compared with the effects of radiation and clinical outcomes in 81 raidcal hysterectomy patients who had also received post-operative irradiation. Sixteen of the 25 patients (64.0%) who died as a result of a recurrence of the disease showed DNA aneuploidy in the radiated areas. In contrast, this was seen in only 6 of the 31 patients (19.4%) who survived without any evidence of recurrence. Before and midpoint paraffin-embedded punch biopsies were used to conduct a flow cytometric DNA analyses in 14 patients who had received radiation treatment only. Prior to treatment, DNA aneuploidy was found in 5 of the 8 radiationresistant patients (62.5%), but in only 2 of the 6 radiation-response patients (33.3%). For the radiation -resistant group, abnormal DNA stem lines continued even during treatment.
In conclusion, the above results indicate that DNA ploidy can be used as a factor for identifying which advanced uterine cervical cancer patients may react favorably or unfavorably to radiation therapy. In addition, based upon changes in DNA histograms during the early stages of treatment, these results imply that DNA ploidy may prove to be an effective method for determining patient' s responses to radiation.View full abstractDownload PDF (3577K) -
Yoshihiko KISHIGAMI, Jing PAN, Sachiko KANAZAWA, Naomi OKA, Sadamu NOD ...1989 Volume 41 Issue 2 Pages 203-208
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSThe authors are conducting an ongoing testing of the screening of cervical smears by flow-cytometer (EPICS C). First, regarding cell suspensions, we rechecked the method of dispersion, fixation and PI-FITC double staining. From this we could get very good cell suspension for measurement with EPICS C. Secondly, as measurment parameters for EPICS C we have adopted a logarithm of intensity of the green (LGFL) and red (LRFL) fluorescence. In addition, LRFL was rectified with 100% subtraction (red-green). Generally, cytoplasms are stained green with fluorescein isothiocyanate (FITC), and nuclei are stained red with propidium iodide (PI).
A large part of the fluorescence spectrum of FITC overlaps that of PI. On the other hand, the wave length specificity of both green and red photodetecter is not so good. So, a system of compensation is required for subtraction of a certain fraction of each fluorescence from the other. In our cases, measured squamous epithelial cells, the cytoplasms are very wide and the nuclei are very narrow. In other words, the green fluorescence is very large in quantity and the red one is very small. So, the leaking of the green fluorescence into the red photodetecter can be a problem. The leaking of the red into the green detecter is negligible. Therefore, we do not need to compensate the green fluorescence quantity, but on the red fluorescence quantity, we regard the value subtracted of the green fluorescence from the red fluorescence as the real quantity of the red fluorescence.
By the above mentioned conditions, we could distinguish each distribution area of cell debris, bacteria, neutrophils and squamous cells on the LGFL-LRFL 2 parameters scattergram measured a cervical smear with EPICS C.
We aim to make this apparatus capable of differentiating malignant cells.View full abstractDownload PDF (4356K) -
A report of three casesYoshiki TAKAHASHI, Masaya HIROSE, Ichiro YAMADE, Tatsuo SUDO, Kazuhiko ...1989 Volume 41 Issue 2 Pages 209-214
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSThree cases of premature of the membranes (PROM) in the second trimester are reported. Long-term oligohydramnios was a factor in all three cases.
In two cases, cervical cervical cerclage combined with fibrin adhesion was perfomed, but this procedure was unsuccessful in preventing leakage of amniotic fluid. Labor started immediately after cervical cerlage combined with fibrin adhesion, and two infants were not saved. Both infants had pulmonary hypoplasia.
In the third case, the mother was treated conservatively with bed rest, tocolytic agents and antibiotics. The newborn infant was delivered by cesarean section at term, and had no respiratory distress, healthy developements, except that the birth weight was only 1, 640gm of severe light for date.
A question must be raised regarding cervical cerclage combined with fibrin adhesion for PROM in the second trimester and oligohydramnios.View full abstractDownload PDF (756K) -
Toshio KIMURA, Yoshiki TAKAHASHI, Kazuhiko KASAHARA, Toshio TENZAKI, A ...1989 Volume 41 Issue 2 Pages 214-224
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSClinicopathological study of endometrial cancer was performed on 36 cases at Shiga University of Medical Science Hospital for eight years since 1979.
The average age of 36 patients was 56.8 year-old and the range 37 to 73 year-old. In 32 of 36 cases, the abnormal genital bleeding was noted as a chief complaint.
In the FIGO clinical stage (preoperative clinical stage), 25 cases were in stage I, 8 in stage II, 1 in stage III, 2 in stage IV. In the surgical stage, 19 cases were in stage I, 5 in stage II, 7 in stage III, 5 in stage IV. The FIGO stage was changed in 10 cases (27.8% : 10/36) after surgery. All ten cases were found in more progressive surgical stage than the FIGO stage. Six of these 10 cases had metastases or invasion to ovaries and tubes. Three of 10 cases had the dissemination in abdominal cavity.
The histological grade and the depth of myometrial invasion correlated with the surgical stage. Patients with the histological grade of G3 were not in surgical stage I and II, and had few the deep myometrial invasion. Patients with surgical stage IV had no histological grade of G1, and had deep myometrial invasion.
The five-year survival rate in the surgical stage was 87.1% in stage I, 57.1% in stage II, 44.5% in stage III, and 0.0% in stage IV. The histological grade and the depth of myometrial invasion had correlation with the five-year survival rate. The histological grade was more correlativility than the myometrial invasion in survival rate. Patients with the histological grade of G1 had 100% of the five-year survival rate.
In cases of surgical stage II or more, no postoperative treatments had a good response. In 9 cases treated by the postoperative chemotherapy, the five-year survival rate was 0.0%.
In conclusion, (1) the FIGO clinical stage was changed in 27.8% after surgery, (2) survival rate was correlated with the histological grade, (3) the postoperative chemotherapy was not successful for advanced endometrial cancer in stage II or more. The most important treatment of endometrial cancer is in the operation, and the method of operation should be selected by the surgical stage.View full abstractDownload PDF (1246K) -
Yoshimasa TSUJI, Kaoru NABUCHI, Yumiko KATOU, Ikuko MORIYAMA, Motohiko ...1989 Volume 41 Issue 2 Pages 225-229
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSNeonatal ovarian cyst in rare, and not easy to detect postnatally.
In recent years, ultrasonographic examination has become well established in obstetrics, and prenatal fetal anomalies are sometimes reported.
This paper presents two different cases of neonatal ovarian cyst.
Case-1 :
Routine ultrasonographic examination was performed at term; a cystic mass was observed on the fetal abdomen, and hydroamnion was disclosed.
At the 38th week of gestation. A 3852 gm female infant was born by Cesarean section.
Soon after delivery dyspnea appeared ; hematoemesis was seen at 12 hrs postnatally.
Intravenous pyelography demonstrated normal kidneys ; CT diagnosis was ovarian cyst.
The cyst was removed on the 8th postnatal day.
The cyst was 8×6×6 cm in size ; histological diagnosis was follicular cyst.
Case-2 :
In the second case, ovarian cyst was detected by prenatal ultrasonic examination at the 36th week of gestation ; the kidneys were normal; there were no abnormal findings in the gastrointestinal tract.
At birth, this infant had a large ovarian cyst, but showed no evidence of gastrointestinal obstruction.
The ovarian cyst was removed on the 16th postnatal day.
The cyst was distorted and showed necrotic change : histological diagnosis was hemangioma.View full abstractDownload PDF (8663K) -
Kenichi BEPPU, Yoshimasa TSUJI, Kaoru NABUCHI, Ikuko MORIYAMA, Motohik ...1989 Volume 41 Issue 2 Pages 231-235
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSHoloprosencephaly is a congenital anomaly characterized by specific types of median facio-cerebral defect.
Facial anomalies include a single eye or some degree of orbital hypotelorism and/or nasal deformities with cleft lip and palate.
Associated brain defects are characterized by arrested prosencephalic cleavage with or without olfactory bulbs.
This paper presents a female case, born at full term via normal delivery ; birth weight was 3506 gm.
Because of facial anomalies and cyanosis, the infant was admitted to our hospital.
Facial anomalies comprised orbital hypotelorism, cleft lip and palate, and proboscis-like nose.
Brain anomalies included hydrocephalus and severe cerebral hypoplasia.
This case was complicated by other anomalies such as TOF and chromosomal anomaly (13-trisomy).
Based on these findings this case was classified as holoprosencephaly, semilobar type, with median cleft.View full abstractDownload PDF (5342K) -
1989 Volume 41 Issue 2 Pages 251-251,253
Published: 1989
Released on J-STAGE: July 05, 2011
JOURNAL FREE ACCESSDownload PDF (5030K)
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